... on ice for 30 min, andcentrifuged at 16 000 g for 30 min. The final supernatantwas assayed for protein concentration and frozen at )80 °C,or processed immediately. All steps were performed ... quaternary isoforms, isoenzymes, monomeric forms;assay for selective cytotoxicity of the enzyme. Methods Enzymol.341, 248–263.13. Kunitz, M. (1946) A spectrophotometric method for the meas-urement ... cellstreated for 24 h with BS-RNase (lane 1), monomeric MSSAE (lane 2),monomeric MSSG (lane 3), MSSAE from a lysate performed in thepresence of 2 mMiodoacetamide (lane 4), BS-RNase from a lysateperformed...