... Drakenberg21Department of Clinical Chemistry, University Hospital Malmo¨, Lund University, Malmo¨, Sweden;2Department of BiophysicalChemistry, Lund University, Lund, SwedenThe interaction of blood ... vicinity of the membrane.We found that the isolated factor X Gla domain exhibitslow affinity binding to PtdSer-containing membranes with aKd of 4.6 lM. This agrees well with the value of 2.4 ... that mutation of Ca2+ligatingamino acids in the N-terminal part of the first EGF-likedomain of factor X influences the amidolytic activity of theintact protein [36]. However, direct interactions...