... of trigger factor, as was found earlier for
trigger factor assisted refolding of GAPDH [24], increase
with increasing concentrations of trigger factor. The t
1/2
of
Fig. 2. Effect of trigger factor ... presence
of CyP.
Ó FEBS 2002 Chaperone and antichaperone activities of trigger factor (Eur. J. Biochem. 269) 4519
effect of the solution conditions on f...
... result of the presence
of negatively-charged residues at P2 and P2¢ that are
not accommodated within the b2-tryptase active site
because of the presence of Asp147 and Asp143
within the S2 and the ... specificity of CG and chymase
Crystallographic data show that the S2¢ subsite of CG
is highly polar as a result of the presence of three posi-
tively-charged residues: Arg4...
... Department of Biology and Biochemistry, University of Bath, UK
2 Department of Biochemistry, University of Wisconsin–Madison, USA
3 Department of Chemistry, University of Wisconsin–Madison, USA
Introduction
Upon ... R
h
F
o
, where F
o
and F
c
are the observed and calculated
structure factor amplitudes of reflection h, respectively. R
free
is equal to R
cryst
for a random...
... structure of cN-II
showing interfaces A and B and the Mg
2+
site. The inset shows
the tertiary structure of each subunit. Effector sites 1 and 2 and
the active site are shown.
Table 2. Effect of point ... transfer of
phosphate esterified in the 5¢ position of 6-hydroxypu-
rine monophosphate nucleosides [1]. The transfer of
phosphate can lead to phosphorylation of inosine,...
... HPRT
and hypoxanthine. (C) PRTFDC1 and
guanine. (D) HPRT and guanine.
Table 2. K
m
and V
max
values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... characterization of numerous com-
plexes of the human HPRT and several bacterial and
protozoan HPRTs have been undertaken [13–17]. The
structure of human HPRT can be divided i...
... performed using
T-COFFEE [44].
Docking simulation of substrate on the active site
of HYD
Js
The substrate binding pockets of HYD
Js
and the mode of
interaction between substrate and enzyme were ... [39].
Cloning and expression of putative HYDs
The genomic DNA of Jannaschia sp. CCS1 and Pseudomo-
nas fluorescens PfO-1 were kindly supplied by Mary Moran
(University of Georg...
... an
accurate measure of the k
off
parameter in CaM-bound
eNOS and nNOS [58], and thus prevented assessment
of the relative importance of conformational change
rates versus rates of FMNH
2
formation ... Model and simulations of cytochrome c reduction by NOS
enzymes. (A) Scheme of cytochrome c reduction. The model uses
four kinetic rates: dissociation (k
1
) and association (...
... & Buckmann
AF (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H
and 4S-H-2NAD(P)H and determination of the stereo-
specificity of 7-alpha-hydroxysteroid and 12-alpha-hy-
droxysteroid dehydrogenase. ... isotope effects using PEG-modified
variants and glycoforms of glucose oxidase: the
relationship of modification of the protein envelope
to C–H activation and tunneling. Biochem...
... and pyridine nucleotides is highly
dependent on the approach and colinear orientation
of the N5 of the flavin, the hydride to be transferred,
and C4 of the nicotinamide. In FNR, displacement
of ... mutagen-
esis of tyrosine-98 in the flavodoxin from Desulfovib-
rio vulgaris (Hildenborough): regulation of oxidation–
reduction properties of the bound FMN cofactor by
aromatic,...
... D and E + F), (b) a few hydrogen
bonds, and a hydrophobic interaction between A + C,
B + E and D + F, and (c) electrostatic forces in the
central channel of the hexamer between B, C and F,
and ... Phe133 of UpUMPK was
mutated to Asn or Ala. The mutant enzymes, F133N
and F133A, were expressed, purified and characterized.
With 1 mm UMP and ATP as substrates, the activities...