Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx

... Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression Ricarda Jahnel1,*, ... B and D of F-11 cells again separated intotwo bands. The slower migrating band always correspondedto the rat brain control, and the faster migrating band to the N18TG2 control (Fig. 5). The ... threshold and it does not respond to capsaicin or protons. Here wedescribe the expression of VRL-1 in the rat dorsal root ganglion- derived cell line F-11, a hybridoma of mouse neuroblastoma (N18TG2) and...

Báo cáo khoa học: An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid b-protein doc

... (calnexin) and cytoskeletonprotein (tubulin) in these cells as the internal controlproteins. The calnexin and tubulin levels were found tobe similar between these cells, conﬁrming that the sameamount ... time betweensynoviolin-null cells and wt cells. As shown in Fig. 2,western blot analysis of the intracellular degradation of NCT in synoviolin-null cells and wt cells followingcycloheximide treatment ... ubiquitination confer speciﬁcity to the ubiquitin system by directly interacting with the sub-strate proteins and helping to transfer ubiquitin tothem. Therefore, to determine whether NCT is the substrate...

Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deﬁciency doc

... explained by the proximity of the mutation to the cleavage site, probably resulting in asmall interference with the binding of the activator to the FXI zymogen.There are some examples of inherited ... (1993) The linkage between binding of the C-terminal domain of hirudin and amidase activity in human alpha-throm-bin. Biochem J 289, 475–480.45 Baglia FA & Walsh PN (1996) A binding site ... activation loop at the N-terminus of the catalyticdomain, are notable. In the zymogen FXI, Ile371 is exposed to the solvent, while in the activated FXI it is inserted into the protein. The picture...

Báo cáo khoa học: "A Feedback-Augmented Method for Detecting Errors in the Writing of Learners of English" docx

... included in the training data. the target errors since they are based on the articles and the distinction between singular and plural.Finally, the tagged instances are stored in a ﬁlewith their ... that the tagging rules can be used only forgenerating training data. They cannot be used todistinguish mass and count nouns in the writing of learners of English for the purpose of detecting1According ... ﬁlewith their surrounding words. Each line of it con-sists of one of the tagged instances and its sur-rounding words as in the above chicken example.2.2 Learning Decision Lists In the proposed method,...

Tài liệu Báo cáo khoa học: "A Multimodal Interface for Access to Content in the Home" pdf

... understanding of system capa-bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the ... and pen input, includ-ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of movies-on-demand and supporting ... completing the same two sets of scenarios and then freely interacting with each system. For counterbalancing purposes, half of the subjects used the tablet and then the re-mote control and the other...

Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx

... a site of interaction between the cytochrome and the quinones by studying inhibition of the nitrate reductase activity by HOQNO. It has thereforeclariﬁed the binding site of the menaquinols, ... fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH2at the QPsite [27].Study of the reduction and oxidation of the cytochromehas ... showing cross-inhibition of menadiol and duro-quinol on the binding of these electrons donors to the cytochrome b of nitrate reductase [13].These results with HOQNO explain those of Rotheryet...

Báo cáo khoa học: Distinct but critical roles for integrin aIIbb3 in platelet lamellipodia formation on ﬁbrinogen, collagen-related peptide and thrombin pot

... as‘inside-out’ signalling) promote a conformationalchange in the extracellular domain, leading to anincrease in afﬁnity, thereby promoting integrin–ligandinteractions [2]. In turn, clustering ... strengths rather than the nature of the ligand medi-ating lamellipodia formation. As shown in the presentstudy, immobilized CRP and thrombin induced amuch greater increase in intracellular ... out-side -in signalling by the integrin is not required forlamellipodia formation on ﬁbrinogen in the presence of thrombin. These results further emphasize the importance of integrin engagement in lamellipodiaformation...

Báo cáo khoa học: H2O2, but not menadione, provokes a decrease in the ATP and an increase in the inosine levels in Saccharomyces cerevisiae An experimental and theoretical approach pot

... from Fig. 1 and Table 1, suggested that (a) the main if not unique source of inosine is the intracellularpool of adenine nucleotides, (b) the decrease in ATP and the increase in inosine, promoted ... accompanied by an increase in the rate of synthesis of Ino, without any need to modify the kineticparameters or activities of the enzymes involved in the pathway from ATP to Ino.Being aware of the simpliﬁcations ... activities of the enzymes involved in the pathway from ATP to inosine, in yeast extracts from(un)treated cells, could not explain the eﬀect of H2O2on the levels of ATP and inosine. Application of...

Báo cáo khoa học: Calpain 1–titin interactions concentrate calpain 1 in the Z-band edges and in the N2-line region within the skeletal myoﬁbril doc

... peroxidase labelling,density increasing of the Z -line, in particular at the edges of the structure, conﬁrms the immunoﬂuorescentstaining. On the other hand, labelling of the Z-bandregion by CP1 ... Consequently, the colocalization of calpain 1 and calpain 3 at N1- and N2 -line levels, the postmortem fractures at the N2 -line level, as well as the implication of titin in the bindingto calpain 3 [20] and ... calpain 1. We conclude that titin affords binding sites to cal-pain 1, which concentrates the protease in the regions restrained by the Z-band edge and the N1 -line as well as at the N2 -line level,...

Báo cáo khoa học: "Event-coreference across Multiple, Multi-lingual Sources in the Mumis Project" doc

... mul-timedia indexing and searching. It is well knownthat there are no effective methods for automaticindexing and retrieving of image and video frag-ments on the basis of analysis of their visual ... Jong** and Yorick Wilks**University of Sheffield, United Kingdom; **University of Twente, The Netherlands***DFKI, Germany; ****MPI, The Netherlands*****University of Nijmegen, The Netherlandssaggion@dcs.shef.ac.uk ... projects therefore have ex-plored the use of collateral textual descriptions of the multimedia information for automatic taskssuch as indexing, classifying, or understanding. The Multimedia Indexing...

Xem thêm