Báo cáo khoa học: Identiﬁcation, structure and differential expression of novel pleurocidins clustered on the genome of the winter ﬂounder, Pseudopleuronectes americanus (Walbaum) ppt

... differential expression of novel pleurocidins clustered on the genome of the winter ﬂounder, Pseudopleuronectes americanus (Walbaum) Susan E. Douglas, Aleksander Patrzykat, Jennifer Pytyck and Jeffrey ... degeneration of the sequences upstream of the pseudogenes made it impossibleto predict the locations of the ﬁrst introns and exons of Y1, Y2andY3.An alignment of the peptides predicted from the pleurocidin ... antimicrobial peptide candidates for bothhuman and nonhuman therapeutants from genomicsequences and will aid in understanding the evolution and transcriptional regulation of expression of these peptides.Keywords:...

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... these stages in the hydrother-mal vent environment and, second, the aposymbiotic-speciﬁc expression condition could be masked by the developmental condition.Comparison with western blots and ... and SDS ⁄ PAGE on cytosolic fractions [14,15] concluded that there weretwo CA proteins: one of 27 kDa in the branchialplume, and another of 28 kDa in the trophosome.From the differential expression ... overexpression approach of RpCAbr and RpCAtr, we cannot know the functionaleffect of changes of some key amino-acids.Origin and number of nonvertebrate CAsAlthough the bootstrap values of the...

Báo cáo khoa học: Identiﬁcation, characterization and activation mechanism of a tyrosine kinase of Bacillus anthracis docx

... loss of the autophophorylation activity(Fig. 1B). The observed changes in the activity of mutants could be due to alterations in the secondary structure of the proteins. The secondary structures ... removes the inhibitory conforma-tion of the activation loop of the kinase, leading to itsphosphorylation and further stabilizing the active form of the enzyme [24,25].In order to estimate the ... measurement of the equilibriumbinding constant Ka, the enthalpy of complex forma-tion (DH) and the complex stoichiometry of a protein–protein interaction without the need for modiﬁcation of the...

Báo cáo khoa học: Identiﬁcation, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot

... pCR-h2.Construction of expression vectors. Inserts were isolatedfrom pCR-h1 and pCR-h2 by digestion with Nde 1and BamHI and ligated into the pET11a expression vectorlinearized with Nde1andBamHI, ... pETHvTrxh1 and pETHvTrxh2, respectively. The sequences of the insertswere determined on both strands and found to be asexpected from the identified TC sequences, and confirmedthat the cloning junctions ... calculation of the protein concentration in the present study.Reduction of BASI. Previously, BASI has been identifiedas a possible target of thioredoxin h, on the basis of itsreduction by recombinant...

Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

... Takentogether, the correlation of fully extended structures fromHep III and the proportion of two-PEtn-containing struc-tures with the expression of the Sial-lNnT unit, wouldsuggest that the addition of ... step and (B) one-dimensional TOCSY-NOESY spectrum using selective excitation of H-11H-resonance of the GlcNAc residue in the TOCSY step and of H-3/H-41H-resonance of the GlcNAc residue in the ... irradiation of the anomeric protonat 4.45 p.p.m. in the TOCSY step, subsequent irradiation of the H-3 proton at 3.73 p.p.m. in the NOESY step revealed the anomeric proton at 4.74 p.p.m. of the b-D-GlcNAcresidue,...

Tài liệu Báo cáo khoa học: Identiﬁcation of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt

... suchG-quadruplex structures in genomic DNA.Materials and methodsPreparation, expression and purification of GSTfusion proteins The EWS cDNA was cloned into the pGEX6P-1 vectorbetween the EcoRI and XhoI ... the high-affinityHtelo binding of RGG3 and RBD. The ability of RGG3 and RBD to repress transcription activation byEAD raised the possibility that RGG3 and RBD block the interaction between the ... vitromethylation of the recombinant EWS with PRMT3showed that PRMT3 is responsible for the asymmetricdimethylations of specific Arg in the RGG region [47].In our study, the methylation of the RGG3...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebraﬁsh, Danio rerio docx

... organization, there is a conservation of syntenybetween the human and zebrafish genomes where the t-bet and foxp3 genes are found. In contrast, there wasno synteny between the human and zebrafish regionscontaining ... preliminary expres-sion data suggest that these genes are involved in the fish immune response, and that there is a relationshipbetween the expression of these transcription factors and the cytokine ... concentrations for 4 h, and the total RNA was recovered. Following reverse transcription, the relative expression of each gene was detected by real-time PCR and normalized to the expression of...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... location was determined tobe the position of the putative transcription start site(Fig. 1B). Analysis of the 5¢ upstream region of exon 1demonstrated the lack of a conserved TATA boxupstream of ... The pBridgeconstructs contain two multiple cloning regions and condi-tionally express two proteins: SmSmad4 in-frame with the Gal4BD, and either SmTbRI or SmTbRI-QD under the con-trol of the ... revealed the interaction of SmSmad4 with SmGCN5 and demon-strated its participation in the formation and, prob-ably, the stabilization of the transcriptional proteincomplex. Figure 9B shows that the...

Tài liệu Báo cáo khoa học: Identiﬁcation of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx

... biosynthesis of triterpenesaponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding theseenzymes. The biosynthesis of triterpene saponins involves the initial ... range of structural diversity produced by asequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini-tial origin of structural diversity of skeletons in their biosynthesis, and ... cyclization of 2,3-oxidosqualene, a common pre-cursor of all the sterol and triterpene biosyntheses, intovarious cyclic triterpenes, followed by oxidative modiﬁ-cation of these carbon skeletons and...

Tài liệu Báo cáo khoa học: Identiﬁcation of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx

... critical contribution of Utr1p to growth in low iron medium. The contributions of the othertwo enzymes were also demonstrated; however, these were observed only in the absence of the critical contributor, ... detected on these solidmedia (data not shown). The expression of POS5, UTR1 and YEF1 via ahigh-copy vector complemented the poor growth of the pos5 cell on SG solid medium and on and in solid and ... defects of the pos5 mutantTo conﬁrm the complementing ability of LEU2 for the growth defects of the pos5 mutant, we examined the growth of pos5 cells containing CgLEU2 instead of POS5 on the chromosome,...

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