Báo cáo khoa học: Comparative analysis of the site-speciﬁc N-glycosylation of human lactoferrin produced in maize and tobacco plants pdf

... Comparative analysis of the site-specific N-glycosylation of human lactoferrin produced in maize and tobacco plants Beńe´dicte Samyn-Petit1, Jean-Pierre ... France;2Meristem Therapeutics, Clermont-Ferrand, FranceWe have compared the site-by-site N-glycosylation status of human lactoferrin (Lf) produced in maize, a monocotyle-don, and in tobacco, used ... species [14,15]. Thesefindings indicate that plants do not exhibit the samepotential of N-glycosylation, according to the level of expression of several key enzymes involved in the initiation(i.e....

Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... simultaneouslyinteracts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the mainprotein body is essential for the activity and, together with the 87–104 ... almostlinear and can be obtained by calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthattheformation of the N-terminal ... was examined on K562 human erythroleukemic cells and A431 human epidermoid carcinoma cells by measuring the incorporation of [35S]methionine into newly synthesizedproteins after 96 h of incubation...

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... probably induced by streng-thening of the interaction between the structural TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the ... TnC-bind-ing site interacts with the C-domain of TnC in both the relaxed and contractile states, which plays a role in maintaining the structural integrity of the troponincomplex [17,18]. These ... and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of bothTnC and...

Báo cáo khoa học: Proteome analysis at the level of subcellular structures Mathias Dreger pot

... assessed in more detail,Rout et al. interpreted the significance of the identification of the other proteins in three ways: firstly, there are proteinsthat according to the literature are known NPC interactors,e.g. ... which, dependingon the approach, contain different kinds of informationexceeding the mere identification of proteins. Thesecomprise of studies on different part of the nucleus of eukaryotic cells ... macrophages. In this analysis, in addition to the description of the phagosome proteome, the maturation of the organelle was monitored by comparative analysis of phagosomes in different stages. The authors...

Báo cáo khoa học: Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin docx

... of Jacalin-related lectins Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins,Castanea crenata agglutinin and Cycas revoluta leaf lectinSachiko ... wasdetermined by measuring the amount of uncoupled protein in the above wash fraction by the method of Bradford [33]. The resulting lectin-columns were slotted into a stainlessholder, and connected ... of nonreducing terminal mannose and C2-OH of redu-cing terminal mannose in Mana1–2Man, than in the case of an ‘equatorial–axial’ linkage, e.g. when Glc-NAcb1–2Man is formed. On the other hand,...

Tài liệu Báo cáo khoa học: Poly(ADP-ribose) The most elaborate metabolite of NAD+ Alexander Burkle pptx

... modulation of chromatin structure and transcription mediated by nucleosome binding of PARP-1 [12], and in the determination of speciﬁcity in a retinoid signalling pathway via direct modulation of Mediator ... embryo-genesis.PARP-3 The protein domain structure of PARP-3 is verysimilar to PARP-2, featuring a small DNA-bindingdomain consisting of only 54 amino acids and compri-sing a centrosome-targeting motif ... found in tankyrase and tankyrase-2 (see below) within the known members of the PARP family. Adjacent to the ANK domain isanother protein interaction motif, the sterile alpha-module. The C-terminus...

Tài liệu Báo cáo khoa học: A role for the intersubunit disulﬁdes of seminal RNase in the mechanism of its antitumor action docx

... mechanism of antitumouraction of BS-RNase is based on the ability of the proteinto resist the neutralizing action of the cytosolic RNaseinhibitor, a resistance due to the dimeric structure of the enzyme. ... devoid of toxicity as native, dimericBS-RNase (data not shown).It is noteworthy that in the inactive MCM, MCA and MAE monomers Cys31 and Cys32, the cysteine residuesoriginally involved in the intersubunit ... hypothesize that the cytotoxicactivity of the latter monomers was due to the presence in these proteins of disulﬁde bonds, with their potentialchemical instability. It is well known that in the...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... 1). These findings can be explained by the coor-dination of l-arginine in the active site of VioC(Figs 4, 5 and 6B). As the stereochemistry of the Ca-atom is crucial to allow the manifold interactionsbetween ... methylene group in the side chain of l-arginine, as exemplified by the binding and catalyticturnover of l-homoarginine. Concerning l-canavanineturnover by VioC, the modified guanidinium group islikely ... chain of bound hAsn in AsnO points towards the centre of the enzyme complex. The differentsubstrate-binding mode results from conformationalcontrol of the enzyme on the side chain rotamer of the bound...

Báo cáo khóa học: Mechanistic insight into the peroxidase catalyzed nitration of tyrosine derivatives by nitrite and hydrogen peroxide doc

... presence of the phenol.Interestingly, these constants resemble the reciprocal of the nitrite binding constants to the proteins.According to Schemes 1 and 2, the nitrating species produced by the ... monitoring the absorbance changes at322 nm due to the formation of the oxidative couplingdimer of 4. The initial rates were determined from the linearpart of the trace at 322 nm. To convert the ... 025% of buffer B (10 mMphosphate buffer, 1MNaCl, pH 6.8) in 20 min, from 2535% in 60 min and from 35100% of buffer B in 20 min, and monitored at 280 and 412 nm. The lactoperoxidasecontaining...

Báo cáo khoa học: "Semantic Retrieval for the Accurate Identiﬁcation of Relational Concepts in Massive Textbases" ppt

... generating name variations of terms in GENA and the UMLS meta-thesaurus1.Table 3 shows the size of the original database and the number of entries expanded by name varia-tions. Terms in MEDLINE ... IE and text mining in biomedical science hasfocused mainly on MEDLINE. In the present pa-per, we target all articles indexed in MEDLINE at the end of 2004 (14,785,094 articles). The follow-ing ... sections explain in detail off-/on-line process-ing for the text retrieval system for MEDLINE.3.1 Off-line processing: HPSG parsing and term recognitionWe ﬁrst parsed all sentences using an HPSG...

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