... underlining and mutagenic changes areshown in bold). BPL-for, 5¢-TTCTTAACCATGGGCTTCAAAAACCTGAT-CTGG-3¢;BPL-rev,5¢-TTAAGGATCCTAAGAACGAGACAGGCTGAACTCTCC-3¢;BCCPD67, 5¢-GTAACCATGGGTGAACAGGAAGA A- 3¢;BCCP-rev,5¢-GGATCCTTAAACGTTTGTGTCTATAAG-3¢; ... BirA[19]. In E. coli, we have expressed active A. aeolicus BPL, the biotin-binding domain of A. aeolicus BCCP as a His6N-terminal fusion (BCCPD67) as well as an A. aeolicus BCCP mutant lacking ... 5¢-GTAACCATGGGTGAACAGGAAGA A- 3¢;BCCP-rev,5¢-GGATCCTTAAACGTTTGTGTCTATAAG-3¢; BCCP K117L, 5¢-GAAGCTCTACTGGTTATGAAC-3¢.DNA was isolated from agarose using a QIAquickÒ GelExtraction Kit, and plasmid DNA was purified using a QIAprepÒ Spin...