Báo cáo khoa học: Human acid sphingomyelinase Assignment of the disulfide bond pattern ppt
Báo cáo khoa học: Human acid sphingomyelinase Assignment of the disulfide bond pattern ppt
... in the absence of a C221-C226 disulfide bond. This proves the presence of the C221-C226 disulfide bond. The appearance of the signals of fragments y¢¢16, y¢¢ 18, y¢¢19 and y¢¢20 2 Dabelow the ... the masses of their reduced forms support the aboveresults.Analysis of the disulfide bonds of haSMasefrom human placentaIn order to confirm that the disulfide bond pattern obtainedfor the recombinant ... [13]. The disulfide bond Fig. 9. Disulfide bond pattern and domain structure of haSMase. The identified disulfide bond structure of haSMase is presented. The N-terminus of native haSMase from human...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... andregulators of other enzymes in the human nucleotidemetabolism (Table S1). The library can then be seen toconstitute the specific metabolome of the nucleotidemetabolism, which would be the most ... interacts with bothO6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved in coordinating ... and3¢OH of the ribose and three water molecules withcoordination distances of approximately 2.4 A˚. The phosphate of the GMP is hydrogen bonded to mainchain and side chain atoms of amino acids...
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Tài liệu Báo cáo khoa học: Topology, tinkering and evolution of the human transcription factor network doc
... and phylogeny. How-ever, the nature of the connections between thesefactors needs to be understood at the level of the pro-tein domain. The global properties of the HTFNtopology are partially ... graph of N ¼ 230 interacting human TFs was obtained (Fig. 1). This can be understood as the architecture of the regulatory backbone. It pro-vides a topological view of the interaction patternsamong ... andappeared isolated. The relatively small size of the con-nected graph compared with all the entries in the data-base might be due, at least in part, to the currentdegree of knowledge of this transcriptional...
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Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt
... changed. (a) In the absence of the K328 side chain, a solvent molecule occupies the posi-tion of the K328 Nfatom (Fig. 4A). The hydrogenbonds and distances imply that the properties of the solvent ... at Ne. The crystal structures of K328 ⁄ K328A–C12 and K328R and the results of the decarboxylation assays of the ‘bulky’ mutants all pointto the orientation of H333 and its hydrogen bond donor ... interesting aspect of the H298E mutation is the orientation of the T300 ⁄ T302 side chains. Both havebeen implicated in the binding of ACP [2], probablyby donating hydrogen bonds to one of the carbonyloxygens...
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Tài liệu Báo cáo khoa học: Human intrinsic factor expressed in the plant Arabidopsis thaliana doc
... calculated per mole of Cbl. Atthesametime,relationofA280to the molar concentra-tion of Cbl in the sample [25] gave the value of e280¼ 61 900ÆM)1Æcm)1, which was quite close to the theoretically ... whichstabilizes the thermo-labile bond between the cobalt atom of Cbl and the amino group. At that point the matrix can beeither used for application or stored in a refrigerator. Beforeadsorption of Cbl-binding ... Approximately one-third of the protein was cleaved at the internal site …PSNPflGPGP. The key properties of the preparation obtained were identical to those of native IF: the binding curves of vitamin B12to...
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Báo cáo khoa học: Human mitochondrial transcription factor A possesses multiple subcellular targeting signals pptx
... fusions) accumulated in the nucleus,suggesting the presence of NLSs. The strength of thesesignals can be ranked on the basis of nuclear ⁄ cyto-plasmic partitioning of the fusion proteins asHMG1 ... interestingpossibility is the regulation of the effects of TFAM byits subcellular targeting. In the mouse and chicken, butnot in the human, expression of a special nuclear iso-form of TFAM was demonstrated. ... dependent upon the upstream methio-nine, which can be provided by a fusion partner. Then,143 bp of hTFAM cDNA encompassing the 5¢-UTR and the first 21 bp of the hTFAM gene was cloned upstream of, and...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... per-centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cellcycle after treatment with 20 lM or 100 lM ATP for 24 h. The gat-ing used for the quantification of the cells in the G0 ⁄ ... after addition of agonist (Fig. 5B).These results further indicate the involvement of bothMAPKs, ERK1 ⁄ 2 and p38, in the signaling pathways,consistent with the inhibition of the antiproliferativeactivity ... detectabledecrease of the p50 form of NF-jB1 in the cytosol after10 min, which is still visible after 60 min (Fig. 5D).Simultaneously with the decrease of p50 in the cytosol,an increase of NF-jB1 p50 in the...
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Báo cáo khoa học: Site-directed enzymatic PEGylation of the human granulocyte colony-stimulating factor pptx
... structure of the 17glutamines present in the wild-type G-CSF. The first column reports the position of the glutamines in the wild-type protein sequence. The second column indicates the percentage of ... in the protein sequence; the overall height of the stack indicates the sequence conservation at that position, and the height of symbolswithin the stack indicates the relative frequency of each ... substrate for the enzyme. However, Mut4 contains other glutamines,and the possibility cannot be excluded that one of the others becomes the PEGylation site. To verify which of the glutamines of Mut4...
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Báo cáo khoa học: Human anionic trypsinogen Properties of autocatalytic activation and degradation and implications in pancreatic diseases potx
... expressed as percentage of trypsin activity measured at the beginning of the incubation. (B) Autolysis in the presence of 100 mMNaCl. (C) Effect of Ca2+ontherelativerateofautolysisinthepresence (s) ... significantly by the ratio of the two isoforms, under conditions that weretypical of the pancreatic juice. This observation suggeststhat the primary trypsin functions, i.e. activation of otherzymogens ... pancreatitis. The human pancreas secretes three isoforms of trypsinogen,encoded by the protease, serine (PRSS)genes1,2and3.On the basis of their relative electrophoretic mobility, the three trypsinogen...
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Báo cáo khoa học: Human proteoglycan testican-1 inhibits the lysosomal cysteine protease cathepsin L pdf
... of residualenzyme activity was used to determine the inhibitionconstant Kidue to the tight binding of the protease by the inhibitor and the possibility of modification of the inhibitorbytheenzyme[32].Thesedatawerefittedtothetheoreticalequation ... comprised of exons 9 and 10. Following the thyropin domain is a regionenriched for acidic residues. Twelve of the 13 amino acids within five amino acids of the C-terminus are negatively charged. The ... of cleavage of varying concentrations of substrate in the presence and absence of testican-1. The intersection of the Lineweaver–Burk plots on the y-axis above the originindicates that cathepsin...
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Từ khóa: Nghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ