Báo cáo khoa học: The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A ppt

... The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A Harry Boer and Anu KoivulaVTT Biotechnology, ... 8.0 and 25 °C. Spectra of the wild-type Cel7A at pH 5.8 (d) and pH 8.0(s), and the pH mutant at pH 5.8 (j) and pH 8.0 (h) were recordedfrom 240 to 190 nm using a 1-mm path length cell and a bandwidth ... to the pH optimum, it had, however, loweredoverall activity on both soluble and insoluble substrates.In order to investigate the thermal stability of T. reesei wild-type Cel7A and the pH mutant...

Báo cáo khoa học: "The Relationship Between Tree Adjoining Grammars " pdf

... the excised subtree is reinserted. The strings in the frontier to the left and right of the foot node appear on the left and right of the frontier of the excised subtree. As shown in the ... MHG's and TAG's is a conse- quence of the similarities between the operations of wrap- ping and adjoining. It is the roles played by the split point and the foot node that underlies this relationship. ... tree 7. The label of the node at which the adjoining operation takes place must be the same as the label of the root (and foot) of ~. The subtree under this node is excised from 7, the auxiliary...

Báo cáo khoa học: "The Tradeoffs Between Open and Traditional Relation Extraction" potx

... as the Web, where the target relations are notknown in advance, and their number is massive. The relationship between standard RE systems and the new Open IE paradigm is analogous to the relationship ... (Culotta and McCallum, 2004) is used. H-CRF also computes the Monge Elkan distance (Monge and Elkan, 1996) between the relations predicted by O-CRF and R1-CRF and includes the result in the feature ... natural thento consider whether the same can be done for all bi-nary relationships.To characterize how binary relationships are ex-pressed, one of the authors of this paper carefully studied the...

Báo cáo khoa học: The proximity between C-termini of dimeric vacuolar H+-pyrophosphatase determined using atomic force microscopy and a gold nanoparticle technique ppt

... the same side of the membrane, and they are close to each other, asvisualized with antibody and gold nanoparticles against 6·His tags onC-terminal ends of the enzyme. The distance between the ... pyrophosphate, and their complexesas substrates and activators of the vacuolar H+-pump-ing inorganic pyrophosphatase (studies using ligandprotection from covalent inhibitors). Plant Physiol ... indicate that the C-termini of the individual subunits of the enzyme are facing the same side of the membrane. The relative positions and proximity of the V-PPaseC-termini on the surface of the reconstituted...

Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot

... Ser218 and the Ser242 peptide backbone and residues within the phosphate binding pocket of 14-3-3 suggested that these may be too great for phospho-S218 and phospho-S242 to bind in the phospho-bindingpockets ... robustly to the dephospho-peptide in contrast to the very low amountsassociating with the phospho-peptide. Therefore, the interaction between centaurin-a1 and CKIa occurswhen CKIa is dephosphorylated.14-3-3 ... that the interaction is phospho-dependent and that the effect was caused by PPasemasking the binding of the peptide to 14-3-3. More of the g and c 14-3-3 isoforms bound to both the phos-pho- and...

Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Puriﬁcation and properties docx

... Eubacteriumacidaminophilum [32] and with the a-subunit of FDH from the thermophilic acetogenic bacterium Moorella thermoace-tica (Clostridium thermoaceticum) (Acc. No. U73807), and itshows about 35% identity with ... latter cases, the polypeptides also reveal the two-domain nature describedabove for Fdh1B.Properties The optimum pH for formate oxidation with NAD+wasdetermined to be between pH 8.0 and 8.5 in ... cofactor determination, the pH of puriﬁedformate dehydrogenase (0.2 mg in 50 mMMops/KOH pH 7.0) was adjusted to pH 2.5 with 2MHCL, then 1% I2/2% KI was added to the acidiﬁed protein at...

Báo cáo khoa học: The transcription factor ZBP-89 suppresses p16 expression through a histone modiﬁcation mechanism to affect cell senescence doc

... prepared and precipitated with antibodies againstFlag and GFP, and detected by using immunoblotting with respec-tive antibodies. Lanes 1, 3, 5 and 7: cells were transfected with HDAC3–GFP and ZBP-89–Flag ... vectors. Lanes 2, 4, 6 and 8: cellswere transfected with HDAC4–GFP and ZBP-89–Flag vectors.Lanes 1 and 2: input and with anti-GFP serum. Lanes 3 and 4:immunoprecipitation (IP) with anti-Flag serum, ... mgÆmL)1o-nitrophenyl-d-galactopyranoside, 2 mm MgCl2 and 100 lLof 2-mercaptoethanol in 200 mm phosphate buffer (pH 6.0), and incubated at 37 °C for 4 h. The absorbance at420 nm was measured after the...

Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf

... in the dominant-negative Vps4p–E233Q mutant protein and tested the ability of the double- mutant proteins to elicit Vps4p mutant phenotypes inotherwise wild-type cells (Fig. 8). Deletion of the ... important. One of these, the TRP sequence, includes the start of the C-terminalhelix as well as the structured loop between the ATPase domain and the C-terminal helix and in the 3D structure ... whether the C-terminal helix and the b-domain DEL sequence are important for the inter-action of Vps4p with other proteins, we tested the abil-ity of the Vps4p mutant proteins to interact with...

Báo cáo khoa học: The Mycobacterium tuberculosis ORF Rv0654 encodes a carotenoid oxygenase mediating central and excentric cleavage of conventional and aromatic carotenoids doc

... mLÆmin)1 with a lineargradient from 100% B to 43% B within 45 min, to 0% Bwithin 1 min, then increasing the flow rate to 2 mLÆmin)1within 1 min and maintaining these final conditions foranother ... v ⁄ v) with the water containing 0.1 gÆL)1ammonium acetate. The column was developed at a flow rate of 450 lLÆmin)1 with 90% A and 10% B for 5 min, to 5% A and 95% B within10 min, then increasing ... preventing the phagosomematuration, which involves the fusion of phagosomes with lysosomes, and by avoiding the development ofan appropriate immune response that could activate the host cell...

Báo cáo khoa học: The impact of G-protein-coupled receptor hetero-oligomerization on function and pharmacology pptx

... to the plasma mem-brane. This work clearly indicates that it is the identityof the activated promoter within the hetero-oligomerthat determines the fate of the internalized receptors.Other ... of the reciprocal inﬂuence of receptorsin the internalization process are the adrenergic a1a and a1breceptors [21], neurokinin NK1 and l opioidreceptors [22], and the b2-adrenergic and ... GPCR–b-arres-tin interaction. (A) The sequential bindingof the ligands to each half of the receptordimer induces the recruitment of twomolecules of b-arrestin and then the activation of ERK. (B)...

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