Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate specificity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx
... PAH and TH (Eur. J. Biochem. 270) 1075
Modeled ligand-protein complexes elucidate the origin of substrate
specificity and provide insight into catalytic mechanisms
of phenylalanine hydroxylase and ... binding to the catalytic domains of PAH and TH
Docking of the native cosubstrate BH
4
into the crystal
structure of the PAH catalytic...
... conclusion, the present studies provide the first
systematic and detailed characterization of the nucleotide
binding specificity of the N- and C-terminal nucleotide
binding sites of the 90-kDa molecular chaperone, ... an intact adenine ring; the stick model is the structure of the
kinked ADP in the Hsp90 crystal, the phosphates pointing out of the
domain;...
... looking at the inflection of the verbs
and number of allomorphs of the stem. Romalo
(1968, p. 5-203) produced a list of 38 verb types,
which she eventually reduced to 10.
For the purpose of machine ... alternations occuring in the stem of irregular
and partially irregular verbs. The system of rules
presented below took into account both the end-
ings pattern...
... bioas-
says specific to the extract. Pure compounds are then
isolated from active fractions and the structure of the
compound is determined. The activity of the chemo-
preventive agent is then confirmed ... focus on IxoA because
of its abundance in the leaves and stems of the plant
and its presence in the fruits.
To examine the mechanism that might account for
t...
... S100–annexin complexes
have a role in the differentiation of gonad cells and neurological disor-
ders, such as depression. These complexes regulate the organization of
membranes and vesicles, and thereby ... Ca
2+
via EF-hand motifs and undergo large conformational
changes. These changes induce the exposure of a
hydrophobic patch at the surface of these molecules...
... that the acetyl group aids
in the stabilization of the helix dipole of the annexin
N-terminus, and therefore the required helical confor-
mation of the peptide. The rmsd for the backbones for
the ... each of the hydrophobic residues are labelled. For example, the side-chain
of L7 of annexin A1 is within 6 A
˚
of the side-chains of L45, A47, F48 within...
... either ‘Regularization’ or DynaLS analysis of the entire
set (¼ 10) of curves. The goodness of the fit by DynaLS
software was evaluated by visual inspection of residual
distribution (random and ... obviates the need of looping of intervening
DNA. The in vivo data supporting the MutS–MutL
foci formation suggests the possibility of extensive
recruitment of protein...
... partly depends on the
kind of data used (e.g. the level of detail and the
amount of inherent ambiguity) and the sampling
method. ‘Geometric’ methods might limit the number
of orientations selected ... microscopy and tomography can also provide
valuable information about the ‘shape’ and organiza-
tion of biomolecular complexes. As these are rather
different kin...
... all-trans retinol
(atROL) as the substrate for the conversion of
11cROL [24–27]; the substrate of RPE65 in the RPE is
atRE [12]. To verify the substrate specificity of zebra-
fish RPE65c, total ... the area of
the 11cROL and 13cROL peaks and expressed as the
mean ± SD of three independent measurements.
Retinal cell dissociation
The zebrafish eyes were enuc...
... stefin A and the different enzymes is the average
distance between the centre of mass of CA atoms of the
stefin molecule and the centre of mass of the CA atoms of
the reactive site cysteine and histidine ... its original position. A comparison of the structure of the unli-
ganded cathepsin B with the structure of the proenzyme, its complexes with...