... Subsequently,
insulin was added, and a stable nonenzymatic rate was
recorded. Finally, PDI was added, and the total NADPH
oxidation rate was recorded.
The oxidase and isomerase activities of PDI were mea-
sured ... reaction times, the refolding
mixture was acidified and immediately analyzed by RP-HPLC. The amounts of native and linear tx 3a were calculated from their elution...
... min at 72 °C
with a 5 min preheat at 95 °C and a 10 min final extension
at 72 °C using primers, S1 (5¢-GCAAATGCAACTGGA
AGCGG-3¢)andA1(5¢-ACAGCCTGCTAGCAAAGA
GG-3¢) for amplification of HRT1, and ... Wititsuwannakul
4
,
Seiji Takahashi
1
, Atiya Rattanapittayaporn
4
and Tanetoshi Koyama
1
1
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Ja...
... MEGFFWKTLLVVGALAIAGTSSLPH.KPLIYEEAVDLAVSIYNSKSGEDS.LYRLLEAVSPPKWD.PLSES
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
L
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
Y
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
A
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
R
L...
... Localization, purification and properties of a tetrathionate hydrolase
from
Acidithiobacillus caldus
Zhanna Bugaytsova* and E. Bo¨ rje Lindstro¨m
Department of Molecular Biology, Umea
˚
University, ... interference of ammonium sulfate with the HPLC-
assay of tetrathionate was also tested. The height of the
tetrathionate peak decreased and a shoulder appeared in the
HPLC-c...
... (5¢fi3¢)
FLAG epitope GAC TAC AAG GAT GAC GAT GAT AAG
DYKDDDDK
Library template
(FLAG-negative)
TTG GGG CTG GAT GAC GCG GAT AAG
L GLDDADK
Randomization NNS NNS NNS GAT GAC NNS GAT AAG
XXXDDXDK
Selected ... proteins carrying a functional
FLAG epitope from an excess of FLAG-negative com-
petitors and from a large library of random variants
was also undertaken by plasmid display, to...
... 5¢-GTGGGCCGCTCTAGGCACCAA-3¢ and 5¢-CT
CTTTGATGTCACGCACGATTTC-3¢; for HPRT, 5¢-GT
AATGATCAGTCAACGGGGGAC-3¢ and 5¢-CCAGCA
AGCTTGCAACCTTAACCA-3¢; for GAPDH, 5¢-ACTCC
ACTCACGGCAAATTC-3¢ and 5¢-CCTTCCACAATGC
CAAAGTT-3¢; ... 5¢-TCTGATGAGACCGTCACTGC-3¢ and 5¢-TC
TCCTGGCTCTCTTTGGAA-3¢; for PAM, 5¢-CCTGG
GGTCACACCTAAAGA-3¢ and 5¢-TGTAAGGACACAC
CGGAACA-3¢; for Y1 receptor, 5¢-CTCGCTGGTTCTCA
T...
... I-D-AEA-K R-DD-QNTIVK-VGATAASASA 122
A
Active site
R101
B
N
R
I/M
HvTrxh1
HvTrxh2
Ta
a
Os
a
Os
b
Td
Ta
b
Ta
c
Ta
d
Hv
a
Sc
Lp
Hb
Os
c
C
K
N
A
Zm
Os
e
Os
d
Lc
Pc
Ata
Atb
Atc
Atd
Ate
Atf
Atg
Bna
Bo
Bnb
Bra
Brb
Pm
Fe
Cm
Nta
Pt
Ath
Ati
Rc
Ntb
Ntc
Pp
Psa
Psb
Psc
Psd
*
Fig. ... purified
protein was missing methionine and alanine from the
N-terminus. This was confirmed by the N-terminal...
... GGGTCTAGAATGGAAAAAGATCTACAGTTAAGA
33 CCGGAATTCTTATTTCCCTTCTCTCATCTC
40 GCGCGCCATGGAAAAAGATCTACAGTTAAGA
41 GGGGGATCCCCATAATCCACTCCACCTGCTAAA
44 GGGGGGGATCCT CATTATTTCCCTTCT
66 AATACCGCCATGTATAATATCTATTACTTC
67 ... AATACCGCCATGTATAATATCTATTACTTC
67 GTAATAGATATTATACATGGCGGTATTGAA
75 GGGGGGGCCATGGAAAGAGCAGACGATTT
4294 H P. Chen et al.(Eur. J. Biochem. 271) Ó FEBS 2004
assay k it was obtained f ro...