... 4-acetamido-4¢-maleim-idylstilbene-2,2¢-disulfonic acid disodium salt (AMS) werepurchased from Invitrogen (Carlsbad, CA, USA). Antise-rum against influenza haemagglutinin (HA) was obtained from Sigma (St Louis, MO). The other antisera ... lipidbilayer independently of the Sec machinery. This is consistent with pub-lished data. Surprisingly, and in conflict with earlier data, YidC is not criti-cal for membrane insertion of MscL. ... Based on the structure of the Mycobacteriumtuberculosis MscL homologue, this position is expectedto be exposed and relatively distant from the mem-brane, and should therefore be accessible...