... subjected toRT-PCR. RT reactions were performed in a total volume of 25 lL containing 1 lg of total RNA, 2.4 lm of oligo-dT,100 U of MMLV reverse transcriptase (Promega, Madison,WI, USA), 1· first ... and therecruitment of MLL1 and H3K4 trimethylation levels are modulated inthe promoter of these Hox genes as a function of their expression. Inaddition, down-regulation of MLL1 results in cell ... Journal compilation ª 2009 FEBS 1629 of homeobox-containing (Hox) genes that are criticalfor cell differentiation and development [13,32,33].Although recent discoveries of HMT activities of MLLs...