... metal–ligand distances (Table 2) compare veryfavorably with data in the protein database, fromwhich an average distance of 2.03 A˚ for Fe–N(His)was inferred [38], and a target distance of ... SupercomputerCenter, San Diego, CA, USA).Metal analysisThis was performed with an inductively coupled plasmaoptical emission spectrometer (Spectro Ciros Vision EOP,Kleve, Germany) calibrated with Fe, ... 000 g), and subsequently analyzed by HPLCusing a Merck-Hitachi La Chrom system (Darmstadt,Germany) equipped with an Aminex HPX-87H column(Biorad, Hercules, CA, USA) as well as with UV and RIdetectors....