... C and lanes 2 and 6 in B),HepG2 treated with 50 nM tetradecanoylphorbol acetate (TPA) for 1, 2 or 6 h (lanes3–7 in A and 3–9 in C, as indicated) or 2 and 6 h (lanes 3–4 and 7–8 in B), and ... EGR-1, Snail and SP-1 in HepG2. HepG2 cells were untreated(con) or treated with 50 nM TPA for 0.5, 1, 2, 4 and 8 h (A and C).Real-time RT/PCR (A) and western blot (C) of EGR-1, SP-1 and Snailwere ... reaction began with annealingof the selection oligonucleotides and the mutagenic oligonu-cleotides to the DNA template, followed by synthesis of themutant strand with T4 DNA polymerase and T4 DNAligase....