... 2 that
delineate the carbohydrate-binding cavity of the lectin
protomer). A closer examination of the structure of
the binding sites indicates that specificity of the Mora-
ceae lectins is primarily ... Structural analysis of the jacalin-related lectin MornigaM
from the black mulberry (Morus nigra) in complex with
mannose
Anja Rabijns
1
,...
... (oligosaccharides from
C-terminal domain or from the whole protein) and using
different equipment. Therefore, the retention of water
can be explained taking into account its strong interac-
tion with the negative ... structures of
the N-linked oligosaccharides present in cruzipain.
HPAEC analysis of the total neutral oligosaccharide
fraction obtained from the C-t...
... these, there is the possibility that, through the
effect of the proximities of the antibody-binding and
Fn-binding sites, the interaction of the repeat with Fn
may be sterically hindered in the ... fibronectin. The
C-terminal domain of fibronectin-binding protein A is organized into 11
tandem repeats, six of which bind the ligand with high affinity; other sites...
... most of the steps in
the biosynthesis of the oligosaccharide portion of the LPS
molecules. The genes lpsA, lic2A and lgtC encode
glycosyltransferase enzymes involved in the addition of
the globoside ... representative
ofthediversitywithinthetypefcluster.
Previous studies of LPS from H. in uenzae have
resulted in a structural model consisting of a conserved...
... molecules of the
cofactors ThDP and Mg
2+
. The ThDP binding sites are
located in narrow clefts at the interfaces formed by the PYR
domains from one subunit and the PP domains of the other
subunit within ... orientation of the aminopyrimidine ring, are
conserved in all ThDP-dependent enzymes. One of these,
the hydrogen bond between the N1¢ atom of the pyrim...
... in ANCE and ACER,
respectively, which in our models are pointing away
from the inhibitor so that the change in the size of the
side chain may have minimal effect on binding. Val956
of C-domain ... studies con-
firm many of the predictions regarding the identity of
the active-site residues and, in the case of tACE, iden-
tify other side chains involved in...
... Considering
flexibility of l oop II in receptor binding, substitution of
Gly33 with Ser (a common residue in other long neurotox-
ins) may lower mobility, thereby affecting the binding
affinity of NTX-1.
Acknowledgements
The ... side chains (such as Arg and Lys, similar to the
corresponding residues in a-CTX that are involved in
receptor binding) would be i n teresting bec...
... behaviour of the serpin.
Complex formation between serpins and their cognate
proteases is fuelled by the thermodynamic properties of the
serpin. Accordingly, insertion of the RCL as s4A and
the ensuing ... linkage of the active
site Ser of the protease to the carboxyl group of P
1
by an
ester bond and insertion of the N-terminal part of the RCL
as strand...
... Five minutes after the sample
had been injected, the concentration of ammonium acetate,
pH 9.0, was increased linearly to 0.2 m in the first 20 min,
Fig. 6. Sugar component analysis of pN4. The ... were
tagged with the fluorophore 2-aminopyridine. Lyophilized
samples were heated at 90 °C for 60 min with 20 lL of pyr-
idylamination reagent, and then heated at 80 °C for 35...
... bond with the backbone CO of F102. Fur-
thermore, the orientation of the side chain of the two
residues brings the guanidinium plane and the aro-
matic ring of F102 into close proximity, with ... only critical
for the stability of the dimer, it may also be involved in
maintaining the integrity of the active site. These results
clearly suggest that the d...