Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc

... LaRonde-LeBlanc et al. Structure and activity of the Rio1 kinase FEBS Journal 272 (2005) 3698–3713 ª 2005 FEBS 3713 Structure and activity of the atypical serine kinase Rio1 Nicole LaRonde-LeBlanc1, ... with the uncomplexed protein. Com-parisons of the structure of Rio1 with the previously determined structure of the Rio2 kinase deﬁned the minimal RIO domain and the distinct fea-tures of the ... 1.60 Structure and activity of the Rio1 kinase N. LaRonde-LeBlanc et al.3700 FEBS Journal 272 (2005) 3698–3713 ª 2005 FEBSFig. 1. The structure and conservation of Rio1. (A) The structure of...

Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens potx

... with the gene of the benzaldehyde lyase and for helpful discus-sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their helpwith data collection and ... comparison of the relativeB-factors along the ThDP molecules shows that the Fig. 4. Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis. The secondary structure ... important for cofactor binding and catalysis. The BAL residues are labeled and the BAL hydrogenbonds are displayed. The yellow and red residues at the top are His70 of BFD and Asn28 of PDC, respectively....

Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx

... shifts for DMT1-TM4 in TFE and SDS.Ó FEBS 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951 Structure and topology of the transmembrane domain 4 of the divalent metal transporter ... structures of DMT1-TM4 inboth SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig. 3. The quality of the ﬁnal structures was assessed using the programPROCHECK-NMR[35]. ... in the presence of Gd3+at pH 4.0, but the degree to which the intensities recovered was lower in the presence of Gd3+,particularly for the C-terminal residues, than in the presence of the...

Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf

... in the mutant. First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... catalysis. The alignment of the small CMLEs from different members of the Rhizobiales with these sequences demonstratedthat in A. radiobacter S2 (and the other member of the Rhizobiales), the amino ... reﬁnement decreased the R-factors by 1% (Rwork) and 0.4% (Rfree) for the P21 structure and by 1.3 ⁄0.6% for the P212121 structure. The ﬁnal R-factors for the models are Structure of Agrobacterium...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... Hence, the maintenance of a condensed chromatin structure throughout the entire body of a gene may rely on the combinedactions of Set3 at the 5¢ end and Set2 at the 3¢ end.In yeast the cycle of ... C (1989) The limits of the DNase I-sensitive domain of the human apolipoproteinB gene coincide with the locations of chromosomalanchorage loops and deﬁne the 5¢ and 3¢ boundaries of the gene. ... Since then there has been an explo-sion of papers on the multitude of chromatin modiﬁca-tions and the factors that can either create orrecognize them. We now have a very detailed picture of the...

Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx

... N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionicinteractions established between Lys11/Tyr13 of ... Likeprophytepsin, the precursor form of cyprosin was processedin different isoforms by the excision of the prosegment and of most of the PSI [21]. Conversely to what has been foundin vivo [31], heavy and ... hybridization, in the digestive glands of the pitchers, the trapping organs of the plant [6]. Usingimmunohistochemistry and immunogold transmission EM,APs purified from the flowers of the cardoon Cynaracardunculus...

Báo cáo khoa học: Structure and function of KH domains docx

... in which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja-cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... single-stranded conformation across one face of the KHdomain, between the a1-helix and the a2-helix and GXXG on the ‘left’, and the b2-sheet and the vari-able loop on the right (Fig. 4A). Together, thesesecondary ... datafrom other KH domains to the KH1–KH2 domains of FMRP. The structure of the tandem KH1–KH2 domains of hFMRP provided the ﬁrst crystallographic description of the structural environment of the Ile304...

Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identiﬁcation of two active site cysteines pptx

... presentstudy, comparison of GlcNAc kinase with a model of the three-dimensional structure of glucokinase provides the ﬁrst picture of the possible structure of the enzyme.Furthermore, two active ... consists of the phosphate1 subdomain and the predicted a-helix I. The other lobe consists of the phos-phate2 and the adenosine subdomains, the predicted b-sheetII and the predicted a-helix III. The ... glucokinase and GlcNAc kinase and the commonATP-binding domain, the GlcNAc kinase was ﬁtted into the glucokinase model using RasMol software. Figure 5 shows the predicted structure of GlcNAc kinase...

Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx

... estimation of the hydrodynamic radii of HAMLET (Rh= 26.9 A˚),which is intermediate of the hydrodynamic radii of the acidic molten globule state of a-lactalbumin(Rh= 20.9 A˚) and the theoretically ... fully understood. The active group of the matrix, the DEAE group, is posi-tively charged and might therefore bind negative mole-cules. At pH 8.5 (the pH of the conversion step) the fatty acid ... describ-ing the form and function of novel complexes such asHAMLET and ELOA, we have provided new evidencethat a loss of native structure can endow proteins and their complexes with distinct and beneﬁcial...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... advantage of p53 mutation analysis, and a unique feature of this database, is the availabil-ity of the residual activity of the majority of p53 mis-sense mutants. The biological activity of mutant ... powerful method, and several algorithms have been developed recently [4–7]. The major drawback of these analyses is the lack of information regarding the activity or loss of activity of the target ... middle group and the high-severity scores and thereby low activity in a thirdgroup, the mutants placed in the ﬁrst and third groupscan be expected to correlate well with high and low activity, ...

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