... TSIPGVYACGDIVTYPGKLPLIVLGFGEAAIAANHAAAYAN-PALKVNPGHSSEKAAPGT 335
E.
coli 277 TSIPGVFAAGDVMDHI YRQAITSAGTGCMAALDAERYLD GLADAK 321
A.
pernix 283 TSIPGIFAAGDCTSMWPGFRQVVTAAAMGAVAAYSAYTYLQEKGLYKPKPLTGLK ... purchased from Toyobo (Osaka, Japan). Emul-
gen 911 was a gift from Kao Chemical (Tokyo, Japan).
NADPH, NADH and NADP
+
were purchased from
Oriental Yeast (Tokyo, Japan). a- Cyano...
... 5¢-AAGTAGGCGGAGTATCGAAC-3¢ (sense) and
5¢-GAAAAAACGCGATCCTACTT-3¢ (antisense). Primers
for unmethylated DNA were: 5¢-GAAGTAGGTGGAGT
ATTGAAT-3¢ (sense) and 5¢-CAAAAAAACACAATCCT
ACTT-3¢ (antisense).
Caspase 3 activity
Cells ... subjected to the caspase assay were seeded on a
24-well plate and transfected with FuGENE 6. The caspase
assay was performed using the CaspACE colorimetric assay...
... 8,
459–467.
10 Kanda A, Iwakoshi-Ukena E, Takuwa-Kuroda K &
Minakata H (2003) Isolation and characterization of
novel tachykinins from the posterior salivary gland of
the common octopus Octopus vulgaris. ... into tachykinin-related peptides, their receptors,
and invertebrate tachykinins: a review. Zool Sci 5,
533–549.
7 Satake H, Ogasawara M, Kawada T, Masuda K, Aoy-
ama M, Min...
... CumOOH, and NADPH
were also obtained from Sigma. Sephadex G-25 was pur-
chased from Pharmacia (Uppsala, Sweden). All the other
materials were of analytical grade and obtained from
Beijing Chemical ... such as
instability, antigenicity and poor availability, much
attention has been paid to its artificial imitation [9,10].
In synthetic approaches, an initial attempt is made
to synthesize...
... OX1 is a Gram-negative bacterium
isolated from the activated sludge of a wastewater treatment
plant, and endowed with unusual metabolic capabilities for
the degradation of aromatic hydrocarbons ... KOH. The
lipo-oligosaccharide was also analyzed after acid treatment,
attained by mild hydrolysis with acetic acid, to obtain
information on the nature of the phosphate and acyl gro...
... NADPH, and glutamate dehydrogenase
were from Wako Pure Chemicals (Osaka, Japan); meat
extract (Extract Ehlrich) w as from Kyokuto Seiyaku Kogyo
(Osaka, Japan); and pentafluorophenylhydrazine was ... and
2-aminomuconic acid in the modified meta-cleavage path-
way (Fig. 1B). The 2-aminomuconate deaminase from s train
AP-3 and that from strain JS45 have been purified and
characterized i...
... of a n a lmost unlimited spectrum of natural
and man-made organic compounds, among them the
tobacco alkaloid nicotine. Perhaps analysed in greatest
detail is the pathway of nicotine degradation ... soil bacteria.
MABO may have evolved from a sarcosine oxidase by
adjustment of the c atalytic centre to a ccommodate the
increased length of the carbohydrate chain.
MABO exhibit...
... especially tree-kernel
approaches are not suitable for Chinese, at least at
current stage. In this paper, we study a feature-based
approach that basically integrates entity related
information ... insufficient training data. Intuitively we can follow
the path of “Nested ⇒ Adjacent ⇒ Separated ⇒
Others” (Nested, Adjacent and Separated structures
are majority in the corpus) to perfo...