Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide ppt
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... into the interaction of human arginase II withsubstrate and manganese ions by site-directedmutagenesis and kinetic studiesAlteration of substrate specificity by replacement of Asn149with AspVasthi ... about 5% of the arginase activity of the wild-type enzyme. As measured by kcat⁄ Km, the catalyticefficiency of the Asn149Asp variant was found to beabout 36-fold lower than that of wild-type ... spite of the rel-atively low agmatinase activity of the Asn149Asp vari-ant, it is clear that the interactions of arginase II withl-arginine and agmatine are greatly altered by replace-ment of...
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Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide. ppt
... maintenance of the axis of polarity. The possible molecularmechanisms responsible for this asymmetry are discussed.Abstracts557 V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) ... presence of Cu(II) generatesreactive oxygen species (ROS) which can hemolyse human red blood cells (RBC). In the present work we examined the effect of sodium azide (NaN3) on RBC in the presence of ... characterized by disturbances of redox and acid-base metabolism. Intravenous laser irradiation of blood (ILIB) is expedient pathogenetically in cerebral ischemia.This work deals with study of mechanism of...
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Tài liệu Báo cáo khoa học: The thioredoxin-independent isoform of chloroplastic glyceraldehyde-3-phosphate dehydrogenase is selectively regulated by glutathionylation docx
... presence of high levels of GSH (datanot shown), suggesting that an oxidoreductase, such asa glutaredoxin, might be required for reduction of themixed disulfide. In this hypothesis, glutaredoxinswould ... understress would allow redistribution of the reducing powerin the chloroplast by decreasing NADPH consumptionand thereby increasing electron availability at the redu-cing side of photosystem I. ... oxidoreductases of the thioredoxin super-family using GSH as the electron donor, appear to bekey regulators of protein glutathionylation [60].Indeed, glutaredoxins are very efficient catalysts of protein...
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Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx
... this case, the lack of binding to the reducedTC is caused rather by loss of the correct three-dimen-sional organization than by disruption of the S-Sbond, per se.The effect of heparin on interaction ... unfractionated heparin at a concentration of 100 unitsÆmL)1.Fig. 2. Binding of anti- (human TC) sera toreduced and unreduced TC in a westernblot. Recombinant human TC produced inyeast (TCy) was ... pairs 4-7 ⁄ 3C4and 2-2 ⁄ 3C4, where binding of the second mAb of thepair was inhibited by < 20%.Interaction of mAbs with the C-terminal domain of human TC was determined using peptides TCp11and...
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Tài liệu Báo cáo khoa học: "A Finite-State Model of Human Sentence Processing" docx
... finite-statemodels of language are crucially limitedin their explanatory power by the local-ity of the probability distribution and thenarrow scope of information used by themodel. We show ... probability.Under this general assumption, the overall diffi-culty of a sentence is expected to be measured orpredicted by the mean size of probability decrease.That is, probability will drop faster ... number of test sets for each ambiguous typeand the number of cases where the model correctlypredicted reading-time penalty of garden-path sen-tences.(12) Ambiguity Type (Correct Predictions/TestSets)a....
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Báo cáo khoa học: The enzyme-binding region of human GM2-activator protein pdf
... location of the ceramide tail of GM2 as identified by crystallo-graphic analysis of lipid complexes of GM2AP [21],which positions the tetrasaccharide head-group of thesubstrate in direct vicinity of ... appropriate amount of HexA were incubated at 37 °C for 30 min in the absenceor presence of various amounts of GM2AP (up to 10 lg).Reactions were stopped by the addition of 5 vol. of a 0.2 mglycine, ... concentration of acceptor vesicles was100 nmolÆmL)1in a total volume of 400 lLin50mm citratebuffer, pH 4.2. The transfer of NBD–GM1 was started by the addition of GM2AP to a final concentration of 37.5...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... T-cellsubsets are expanded by interaction with SSA. Someauthors indicated clonal expansion of T -cells bearing human Vb3, Vb12, Vb17 and Vb19 [20]. Others showedproliferation of human T -cells bearing Vb1, ... SSA have affinity for human Vb5.2 and V b1withKd of 9–11 lMwith a fast kassand a moderately fast kdiss. In spite of the reported stimu-lation of V b2.1 bearing T -cells by SSA, we o bserved ... capacity with human peripheral blood mononuclear cells (PBMCs). Thebinding of SSA and mutant C26S to soluble TCR b-c hainmolecules Vb1, V b2.1 and V b5.2 was measured in real time by a Ôresonant...
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Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot
... view of selected residues of hamster NAT2. Residues are colored by atomtype: carbon, nitrogen, sulfur and oxygen in white, blue, orange and red, respectively.X. Zhou et al. The catalytic role of ... perturbation of the active site by complete removal of the side chain mainly affectedenzyme acetylation (k2).pH dependence of NAT acetylationUsually, the pH dependence of acetylation of NAT(k2⁄ ... acetylation by AcCoA (Eqn 2), the values of k2for AcCoA can be predicted to range from 10 to1740 s)1. Hence, from the kcatvalues for the acetyla-tion of PNA by AcCoA, we were able to predict...
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Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt
... involved affinity purification of profilin-actin complexes on poly(l-proline) Sepharose, enrichment of actin by a cycle of polymerization and depolymerization,isoform separation by hydroxyapatite chromatography, ... mutant of M90T cured of the large virulence plasmid. Shigella strainsSC300 and SC301 contain plasmid pIL22 encoding anafimbrial adhesin of uropathogenic E. coli [54].Shigella infection of HeLa cells Infection ... solutionPurification of Iba2 was always performed in thepresence of a reducing agent, dithiothreitol.SDS ⁄ PAGE without prior reduction confirmed theFig. 1. Sequence alignment of human Iba1 [4] and...
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Báo cáo khoa học: Tyrosine-dependent basolateral targeting of human connexin43–eYFP in Madin–Darby canine kidney cells can be disrupted by the oculodentodigital dysplasia mutation L90V ppt
... confirm thataddition of 10 mm sodium butyrate does not alter the polar-ized membrane properties of MDCK cells (Fig. S1). Cells were kept on ice for the duration of the experiment. Cells were rinsed ... oculodentodigital dysplasia (ODDD), a rare human developmental disorder characterized by defectsin the craniofacial bones, loss of tooth enamel, andabnormal soft tissue separation of two or three digits[24,25]. ... panels indicate the location of the Z sections. WT Cx43–eYFP was expressed on the basolateral membrane domain of MDCK cells. (D) Z section of a monolayer of uninfected MDCK cells showing background...
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