... glycine is nonconservative, we introduced alanineat these two positions, with the aim of determiningwhether any of the observed effects were a result of changes in the size and charge of the ... structure, observed in the CD spectrum,affects this part of the protein, or whether there istransient unfolding of the end of this helix. There areexamples of helices in proteins that undergo ... differences between wild-type enolase and the variants, indicating that the variants were folded cor-rectly. However, there were significant differences in the aromatic region. The spectrum of the...