Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... time is required.Instead of expressing the oraE and oraS genes separately, the DNA fragment containing both genes was cloned i nto the same expression vector under the control of the T7promoter, ... Comparison of t he deducedamino acid sequence of oraE to those of known PLP- dependent aminomutases reveals the presence of a con-served PLP-binding site, a lysine residue at position 629, at the ... the mutant protein were the same as those of the wild-type.Protein determination and enzyme assayProtein concentrations were determined by the method of Bradford using bovine serum albumin as standard...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... preparation showed only one bandwhen stained by GelCode Blue Stain Reagent and SilverXpress Silver Staining Kit in SDS gel electrophoresis(Fig. 1).Isoelectric focusing in the 2D gel detected a single ... excision and N-terminal sequence analysis byEdman degradation. The N-terminal sequence was deter-mined in the Protein Analysis Center, Karolinska Institutet(Stockholm, Sweden).PAGESDS/PAGE ... single spot and the pI of the tetrathionate hydrolase was 9.8. The molecular mass of the purified tetrathionate hydrolase wasdetermined by SDS electrophoresis and specified byMALDI-TOF MS. The enzyme...
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Báo cáo khoa học: Expression, purification and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog potx
... methionine residues, as suggested byMS/MS. The MS/MS sequencing also confirms the absence of the N-terminal Met1 residue of the a-subunit, in agreement with the predicted cleavage of the Met1-Gly2sequence ... of these peaksare exactly the s ame for the protein recovered from crystals.However, the molecular masses for these two subunits predicted from the amino acid sequence o n the assumption of ... fromasparagine the ammonia that i s necessary f or proteinsynthesis. There are two groups of such proteins, calledpotassium -dependent and potassium-independent aspara-ginases. We have reported...
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Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
... positions, while residues of the secondary sites are indicated by dots. In the topline, asterisks highlight the P1 and P¢2 sites. Residues conserved in at least 50% of the aligned sequences ... determiningtheir protease-specificity) are found in a short segmentcontaining the second conserved cysteine, a secondary sitecontacting the target proteases includes residues adjacent to the ... lies in the presence of a Trp-residue at the P¢2 site of the inhibitor. In the case of Kunitz-domains it is now well established that the primarysites interacting with the target proteases (and...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... observed in cells treatedwith cisplatin [50]. Interestingly, expression of geneswith stronger promoters was strongly inhibited,whereas some genes possessing weaker promoters wereinduced. It was ... pPGM4 were used as the sequence-specific competitors, and changes in p53(1–363) binding to the 32P-labeled 50-mer targets were fol-lowed. We first tested the effect of the presence of the competitor ... chromatin domainon the other. Moreover, some of the p53 downstreamgenes possess more than one p53 response element, and these may differ in their susceptibilities to cis-pla-tination (for example,...
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Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classification pptx
... certain in the case of invertebrates and basal metazoans. Nevertheless, the amino acidsequence of the Sycon group I PLA2demonstrates that the ancestor of group I PLA2 s possessed the same ... purified from the milking exudates of several U. crassicornis specimens. On the basis of the N-terminal sequence of the isolated protein, itscDNA was isolated, sequenced, and recombinantlyoverexpressed ... fordeduced amino acid sequences to measure the extent of sequence divergence. When many divergent sequences arebeing analyzed, and the number of positions used is rela-tively small, the uncorrected...
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Báo cáo khoa học: "Hedge classification in biomedical texts with a weakly supervised selection of keywords" doc
... is that,as we said earlier, hedging is expressed via key-words in natural language texts; and often severalkeywords are present in a single sentence. The seed set S speccontained either suggest ... of these were the kind of phrases that had no unigram components of themselves in the feature set, so these could be re-garded as meaningful standalone features. Examples of such speculative ... the classification of sentences for either speculative ornon-speculative assertions, and extend their method-ology in several ways. Thus given labeled sets S spec and S nspec the task is to train...
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Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot
... consideration in further investi-gations of the role of this cytokine as an LPS mediator. The present PEC culture also showed the advantage of usingmixed-cell cultures for the investigation of ... 2003confirmed that the anti-(IL-18) Ig used in the present studydoes not inhibit the antigenicity of IL-12 by ELISA and that the same results as shown in the present study are obtainedeven when another ... (data not shown).Effect of nonadherent cells in the PEC of HeN miceon the production of NO and cytokines in responseto LPS and its mediators In the preparation of the PEC used in this study,...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
... state. However, there is no clear match of the observed mass peaks to the predicted propeptidesexpected to originate from cleavage of signal peptides.Instead, further processing has been observed, ... controlled culture conditions,different strains of this taxon displayed different MSfingerprints. Because of the ease and speed of the analysis, we investigated whether the hydrophobinclass II ... they are rather speculative, the interpretedmasses agree with verified cleavage sites observed forHFB1 and HFB2 and known sites for signal peptidas-es and Kex2-type peptidases (Table 1). Verification...
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Báo cáo khoa học: Functional classification of scaffold proteins and related molecules pptx
... proteins and their kin. Representatives of the four categories of scaffold proteins and their relatives are presented. The length of the human (unless indicated otherwise) isoform is given, along ... reviewed in two of the other minireviews in this series [5,7]. Here we pro-vide a short overview of their most notable examples,to demonstrate that the distinction between these twocategories is ... however, also makes itinherently limited, because of the necessary neglect of many details. If such exceptions to the ‘rule’ of the system become too numerous, the system has to beimproved...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ