Báo cáo khoa học: Expression, purification and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog potx
Báo cáo khoa học: Expression, purification and catalytic activity of Lupinus luteus asparagine b-amidohydrolase and its Escherichia coli homolog potx
... analysis of isoaspartyl peptidase activity of (a) EcAIII and (b) LlA.3226 D. Borek et al. (Eur. J. Biochem. 271) Ó FEBS 2004 Expression, purification and catalytic activity of Lupinus luteus asparagine ... the expression, purification, and biochemicalcharacterization of two homologous enzymes, with amido-hydrolase activities, of plant (Lupinus luteus potassium-independent asparaginase, LlA) and ... successfulexpression andpurification of twoamidohydrolytic enzymes,from Escherichia coli (EcAIII) and from Lupinus luteus (LlA), as well as their biochemical characterization. Theexpression levels in E. coli...
- 12
- 407
- 0
Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
... Expression, purification and characterization of the secondKunitz-type protease inhibitor domain of the human WFIKKN proteinAlinda Nagy, Ma´ria Trexler and La´szlo´PatthyInstitute of ... in ovary, testis and brain, the most significant expression of the WFIKKNgene is observed in adult pancreas, liver and thymus.In view of the presence of WAP-, Kazal-, Kunitz- and NTR-modules ... (Bubendorf, Switzerland). p-Nitrophenyl-p-guanidinobenzoate was a product of Fluka (Buch,Switzerland).Cloning and expression of the second Kunitz-typeprotease inhibitor module of human WFIKKN...
- 7
- 292
- 0
Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... complete distribution of consensus secondary structure estimated from the model isas follows: 39% of a-helices, 9% of b-sheets, 12% of turns,21% of random coil and 19% of other structures. Theseestimations ... mammalianHDC catalytic site and those of the other group IIL-aminoacid decarboxylases: (a) HDC is the least efficient enzyme of its group; (b) mammalian HDC activity is less sensitive to thepresence of ... T., Kubota, H., Hayashi, H. & Wada, H.(1984) Purification of histidine decarboxylase from the liver of fetal rats and its immunochemical and immunohistochemicalcharacterization. J. Biol....
- 12
- 409
- 0
Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... 5), and the influence of Cu2+ions (Table 3). Maximum activity was found at pH 3 and between 40 and 45 °C. Copper at 0.01 mMgreatly stimu-lated the activity. The presence of Fe2+,Mn2+ and ... production of p-nitrophenol was measuredspectrophotometrically at 405 nm. One unit of the acidphosphatase activity was defined as the amount of proteinwhich catalysed the formation of 1 lmol of p-nitrophenol ... solutiondemonstrated a 17-fold purification and about 8% recovery of the enzyme activity (Table 2).This active enzyme preparation showed only one bandwhen stained by GelCode Blue Stain Reagent and SilverXpress...
- 9
- 609
- 0
Báo cáo khoa học: Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli potx
... Coexpression, purification and characterization of the E and S subunits of coenzyme B12 and B6dependentClostridium sticklandiiD-ornithineaminomutase in Escherichia coli Hao-Ping ... Clostridium sticklandiicomprises two strongly associating subunits, OraS and OraE, with molecular masses of 12 800 and 82 900 Da.Previous studies have shown that in Escherichia coli therecombinant ... theconstruction, coexpression, and purification of the apo-enzyme of D-ornithine aminomutase, together with thetemperature effect o n p rotein expression and characteriza-tion of the recombinant...
- 5
- 401
- 0
Báo cáo khoa học: DmGEN shows a flap endonuclease activity, cleaving the blocked-flap structure and model replication fork docx
... BF2 and BF3 – were 15,17 and 19 bp, respectively (Fig. 7A). The single-stran-ded region of the BF structure was 19 bp, and the gapsizes of the blocked strand of BF1, BF2 and BF3 were4, 2 and ... The N and I domains of DmGEN weresimilar to those of XPG ⁄ class I (Fig. 2A,B). TheN domain of DmGEN showed 35.1, 25.0 and 10.9%homology (% identity) with the N domains of HsXPG, HsFEN-1 and ... representation of the conserved N and I domains of some members of the RAD2 ⁄ XPG family. The total number of amino acids in each protein and homology (% identity) between DmGEN and other members...
- 14
- 433
- 0
Báo cáo khoa học: DYNLL/LC8: a light chain subunit of the dynein motor complex and beyond pptx
... design and use of multivalent ligands and inhibi-tors. Angew Chem Int Ed 37, 2754–2794.38 Barbar E, Kleinman B, Imhoff D, Li M, Hays TS &Hare M (2001) Dimerization and folding of LC8, ... p53–p21 and JNK pathways [91].LC8 interacts with the transcription factor TRPS1 and suppresses its transcriptional repression activity [92].LC8 binds to the N-terminal disordered domain of ERa and ... scaffold. Its flexibility is modulated by long-range couplingbetween DIC dimerization and the binding of lightchains and other components of the motor complexincluding the heavy chain and p150Gluedsubunit...
- 17
- 573
- 0
Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf
... biochemi-cally, and to determine the structure of RMD to better understand whatdetermines substrate specificity and catalytic activity in these enzymes. Weused capillary electrophoresis and NMR analysis of ... RMD monomer. The cofactor-binding domain and the substrate-binding domain are shown in aqua and light sand, respectively; the APPR portion of the cofactor (dark gray) and the ligand analog GDP-D-Man ... aeruginosa, and the structural characterization of RMD from A. thermoaerophilus.Results Purification and stability of His6-GMD and His6-RMDPaWe purified N-terminally His6-tagged fusions of P. aeruginosa...
- 15
- 402
- 0
Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
... participates of H2O2-mediated signaling proces-ses, including regulation of gene expression [27–30].Therefore, we have evaluated possible influences of cTPxI and of the functional state of the ... presence of H2O2 and thereby it interacts with the target genes [11,46,47].This retention is dependent of the oxidation of Yap1cysteine residues by H2O2, that modifies its conforma-tion and ... comprises a small number of antioxid-ant enzymes, but several heat-shock proteins, meta-bolism enzymes and proteases, and is involved withactivities of the ubiquitin and proteasome degradationpathways...
- 12
- 363
- 0
Báo cáo khoa học: Geranylgeranyl reductase involved in the biosynthesis of archaeal membrane lipids in the hyperthermophilic archaeon Archaeoglobus fulgidus potx
... group of GGGP and GGPP. These facts strongly suggest the involvement of the enzyme, A. fulgidus GGR, in the biosynthesis of membrane lipids.ResultsRecombinant expression and purification of an ... volume of 100 lL,430 pmol of [1-14C]IPP (2.1 GBqÆmmol)1), 1 nmol of (all-E)farnesyl diphosphate, 0.2 lmol of a-glycerophosphate,2 lmol of MgCl2,2lmol of sodium phosphate buffer,pH 5.8, and ... reduction of a geranylgeranyl orprenyl group, they and A. fulgidus GGR might havedistinct substrate specificities and physiological roles.The activity of the enzymes on the unknown precur-sors of...
- 10
- 413
- 0
Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDENghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)chuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP