Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... we describe the isolation of Xenopus HIVEP1 ,aswell as partial cDNAs of HIVEP2 and -3. Analysis of the tem- poral and spatial expression of the XHIVEP transcripts during early embryogenesis revealed ... combinations of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of...
Ngày tải lên : 30/03/2014, 13:20
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... comprising the two sugar binding pockets in the pulchellins (Fig. 3). In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-...
Ngày tải lên : 18/02/2014, 16:20
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... samples, and were used for the quantification of the enzymatically formed H 2 S. Measurement of pyruvate formation The activity of the purified d-CDes enzyme was followed through the measurement of the ... and decreased at the beginning of the light cycle. The d-CDes gene expression or the stability of the d-CDes mRNA was negatively regulated by light (Fi...
Ngày tải lên : 16/03/2014, 18:20
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx

... detailed analysis of the 12F-11 paratope and assessment of the varying contributions of CDR and framework regions clearly requires definitive structural data; this analysis is currently in progress. The isolation ... (1999) Mutational pattern of the nurse shark antigen receptor gene (NAR) is similar to that of mammalian Ig genes and to sponta- neous mutations in evo...
Ngày tải lên : 17/03/2014, 10:20
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... intensity of the bands may be the result of a different copy number of the genes in the hybridizing fragments. If so, the number of the bands do not represent the family size. Thus, the mlp gene family ... of the mlp gene. Nevertheless, taking into account the possible size of the mlp gene family and the number of gene variants considered...
Ngày tải lên : 07/03/2014, 15:20
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... experimentally. Indeed, the presence of Arg213 along with the proximity of the charge relay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytic histidine. ... [23]. Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular ma...
Ngày tải lên : 07/03/2014, 16:20
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Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... role for these residues on the catalysis and zinc ions, respectively. These effects may be necessary either for the activation of the catalytic water molecule or for the stabilization of the substrate–enzyme ... examined the V. alginolyticus PepD protein. In the present study, we present the cloning and expression of the V. alginolyti- cus pepD gene, the purifica...
Ngày tải lên : 23/03/2014, 06:20
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt

... considering the oxidation of the substrate by T. reesei TYR2. The closer to the hydroxyl group of phenol the amino group was, the slower was the oxida- tion of the substrate. For most of the substrates ... these states are alternated, has been proposed [16]. The met state is the resting state of the enzyme, and in the absence of substrate about 85–90%...
Ngày tải lên : 19/02/2014, 06:20
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... stresses. The exist- ence of a large family of CIPK and CBL genes has been reported in Arabidopsis and rice [15]. However, the details of the role of these proteins and the inter- Stress-induced CIPK ... showing the nuc- leus, and Fig. 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respect- ively. Discussion Nature h...
Ngày tải lên : 19/02/2014, 07:20
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... evidence for existence of two distinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro. Materials and methods Cell culture and animals Rainbow trout ... 10.0; GAPEXT ,0.05; GAPDIST ,8; MAXDIV , 40; ENDGAPS ,off; NOPGAPS ,off; NOHGAPS ,off.Graphical output of the bootstrap figure was produced by the program TREEVIEW . Isolation...
Ngày tải lên : 19/02/2014, 12:20
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