... we describe the isolation of Xenopus HIVEP1 ,aswell
as partial cDNAs of HIVEP2 and -3. Analysis of the tem-
poral and spatial expression of the XHIVEP transcripts
during early embryogenesis revealed ... combinations of degenerate and specific primers
and by rescreening the cDNA libraries. The partial clones
of XHIVEP2 and -3 covered 5.9 and 3.2 kb of...
... comprising the two sugar
binding pockets in the pulchellins (Fig. 3).
In order to compare the similarity of the A- and
B-chains of the four isoforms, a pairwise alignment
was performed and the values of ... Taken together with the inability of P III
and P IV to inhibit hemagglutination in vitro in the
presence of this sugar, these data suggest that the bind-...
... samples, and were used for the
quantification of the enzymatically formed H
2
S.
Measurement of pyruvate formation
The activity of the purified d-CDes enzyme was followed
through the measurement of the ... and
decreased at the beginning of the light cycle. The
d-CDes gene expression or the stability of the d-CDes
mRNA was negatively regulated by light (Fi...
... detailed
analysis of the 12F-11 paratope and assessment of the
varying contributions of CDR and framework regions
clearly requires definitive structural data; this analysis is
currently in progress.
The isolation ... (1999)
Mutational pattern of the nurse shark antigen receptor gene
(NAR) is similar to that of mammalian Ig genes and to sponta-
neous mutations in evo...
... intensity of the bands
may be the result of a different copy number of the genes in
the hybridizing fragments. If so, the number of the bands do
not represent the family size. Thus, the mlp gene family ... of the mlp gene.
Nevertheless, taking into account the possible size of the mlp
gene family and the number of gene variants considered...
... experimentally. Indeed, the
presence of Arg213 along with the proximity of the charge
relay Asp260 within the active site could be responsible for
the modification of the catalytic properties of the catalytic
histidine. ... [23].
Results and Discussion
Cloning and expression of Rv1399c
The Rv1399c gene product encodes a 318 amino acid
protein with a molecular ma...
... role for these residues on the
catalysis and zinc ions, respectively. These effects may be necessary either
for the activation of the catalytic water molecule or for the stabilization of
the substrate–enzyme ... examined the
V. alginolyticus PepD protein. In the present study, we
present the cloning and expression of the V. alginolyti-
cus pepD gene, the purifica...
... considering the oxidation of the substrate by
T. reesei TYR2. The closer to the hydroxyl group of
phenol the amino group was, the slower was the oxida-
tion of the substrate. For most of the substrates ... these states are alternated, has been
proposed [16]. The met state is the resting state of the
enzyme, and in the absence of substrate about 85–90%...
... stresses. The exist-
ence of a large family of CIPK and CBL genes has
been reported in Arabidopsis and rice [15]. However,
the details of the role of these proteins and the inter-
Stress-induced CIPK ... showing the nuc-
leus, and Fig. 7C, F, K and N are the merged images
of A and B, D and E, I and J, and L and M, respect-
ively.
Discussion
Nature h...
... evidence for existence of two
distinct HSF1 isoforms in rainbow trout and the formation
of heterotrimers of these isoforms in vitro.
Materials and methods
Cell culture and animals
Rainbow trout ... 10.0;
GAPEXT
,0.05;
GAPDIST
,8;
MAXDIV
,
40;
ENDGAPS
,off;
NOPGAPS
,off;
NOHGAPS
,off.Graphical
output of the bootstrap figure was produced by the program
TREEVIEW
.
Isolation...