Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... nonlipolytic hydrolase; Rv139 9c; tuber-culosis. The recent elucidation of the complete sequence of Myco-bacterium tuberculosis genome [1] has offered new perspec-tives for the search of novel drugs ... capture the hydrogen of the catalytic serine for an efﬁcient nucleophilic attack of the substrate ester bond by the serine alcoholate. The shape of the titration curve of the catalytic histidine ... the mutation of Arg213 ﬁ Ala. The oligonucleotides u sedwere: 5¢-gcgccaatcctggacgctgacgtcatcgacgcg-3¢ (forward) and 5¢-cgcgtcgatgacgtcagcgtccaggattggcgc-3¢ (reverse). The bases in bold indicate...

Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf

... pLac1-Bwas selected to characterize the recombinant laccase fromA. niger .Immunodetection of the recombinant laccase and expression of the corresponding gene inA. nigerProduction of the r ecombinant ... was performed by GenomeExpress (Grenoble, France). Expression vectorsTwo expression vectors were constructed using a PCRcloning approach, and the cloned PCR products werechecked by sequencing. ... electron acceptorfrom the reducing substrate and electrons are transferredfrom this copper to the two-electron acceptor type-3 copperpair center [10,11]. Then, t he trinuclear center, which...

Báo cáo khoa học: Expression and characterization of the bioﬁlm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot

... sequence primers obtained from the core region sequence. The nucleotide sequence of the V. alginolyticus pepD gene has been deposited in the GenBank database (accession number DQ335448).Production ... role in PepD Oligonucleotide sequence (5¢-to-3¢)H80A Zinc binding GTGCTTCAAGCAGCGATCGACATGGTGCCACD82A Catalytic GCACACATCGCCATGGTGCCACAAAAGAACGD119A Zinc binding CGCTCGGGGCAGCTAACGGCATCGGCATGGCD119X ... similaritiesin the range 13–34%.Despite the lack of detectable sequence homology,putative active-site residues for catalysis were relativelyconserved in PepD and related di-zinc enzymes in the M20...

Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt

... UV-spectra of the nuclease-treated and untreated preparations revealedthat both of them were contaminated with nucleicacids. Calculation of RNA content showed that the nuclease treatment reduced ... identiﬁed.Considering the long evolutionary distance betweensponges and vertebrate lineages, the elucidation of the function of the 2-5A synthetase in these invertebrates,particularly in the ... the nuclease diges-tion). The results showed that the addition of the nuclease caused the precipitation of the material in aconcentration-dependent manner. Formation of the precipitate in the...

Báo cáo khoa học: Expression and characterization of cyanobacterium heme oxygenase, a key enzyme in the phycobilin synthesis Properties of the heme complex of recombinant active enzyme potx

... in the presence of ascorbate are depicted in Fig. 7, panel A.Addition of ascorbate to the heme–Syn HO-1 complexcommences the reaction, which is monitored by the steadydecrease of the Soret and ... NADPH cytochrome P450 reduc-tase, forming detectable intermediates, the oxy-heme and verdoheme complexes. However, the overall reaction rate of heme conversion is relatively slow. Characteristics ... complex appears to be quite inefﬁcient in the heme–SynHO-1 complex. We have observed that the presence of 30equivalents of ascorbate together with reductase in the reaction mixture avoids the retardation...

Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt

... P2Y1 and P2Y2receptorsare both coupled to phospholipase C [8].Whereas the presence of the P2Y2 receptor in glioma C6 cells is generally accepted, the presence of the P2Y1 receptor, which ... consisting of six measurements repeatsfrom three experiments. Zero time represents the ATP concentra-tion in the culture medium containing serum, before the experi-ment. Broken line, observed increase ... changes are notconnected with differentiation of the cells into astro-cytes or oligodendrocytes, but are connected with the lack of serum in the culture medium. Furthermore,these data indicate that...

Báo cáo khoa học: Expression and characterization of soluble forms of the extracellular domains of the b, c and e subunits of the human muscle acetylcholine receptor pot

... we present the expression and charac-terization of the ECDs of the b, c and e subunits of the human muscle AChR. We describe their expression in a soluble, glycosylated form and in satisfactoryamounts ... of the ECDs of the non-a sub-units of human muscle AChR for use as starting material for the determin-ation of the 3D structure of the receptor ECDs and for the characterization of the speciﬁcities ... on the yield of c1 –128 was considerably counteracted by the addition of the FLAG epitope, and (c) the pres-ence of the proline residue did not improve expression, we proceeded to large-scale expression...

Báo cáo khoa học: Expression, puriﬁcation and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot

... protein sequencingsystem showed that the elastase cleavage occurred at the boundary of the b-galactosidase region of the b-gal fusionprotein. The amino acid sequence of the resulting puri-ﬁed ... segmentcontaining the second conserved cysteine, a secondary sitecontacting the target proteases includes residues adjacent to the fourth conserved cysteine ([18] cf. Fig. 3). Among all the contact ... dichroism spectra of the second Kunitz–typeprotease inhibitor module of human WFIKKN. The solid line indicates the spectrum of the recombinant protein, the dotted line indicates the CDPro-predicted...

Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt

... paracrine rolesZongwei Li, Hua Wei, Linzi Deng, Xiangfeng Cong and Xi ChenResearch Center for Cardiac Regenerative Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, ... proliferation and type I and III collagen expression, exerting paracrine anti-ﬁbrotic effects.However, researchers did not analyse the active compo-nents of the conditioned medium [6]. Other researchershave ... wasreplaced every 48 h. The MSCs used in subsequent experi-ments had been passaged one to three times. All procedureswere approved by the Animal Care Committee of the Cardiovascular Institute and...

Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

... measured the accurate mature amino acid sequences of the fourcomplex II subunits by RT-PCR and N-terminalsequencing. The nucleotide sequences of the four por-cine complex II subunits were ... were sequenced correctly(Fig. 4), with the exception of the 5¢-end and 3¢-endprimer sequences from human mitochondrial com-plex II (see Experimental procedures). However, when the recombined ... determination, we had to measure the gene sequences and full amino acid sequences of the porcine complex II subunits. An abundance of gene sequences of mammaliancomplex II in the GenBank database was helpful...

Xem thêm