Báo cáo khoa học: Mechanical regulation of the Cyr61/CCN1 and CTGF/CCN2 proteins Implications in mechanical stress-associated pathologies pptx

Báo cáo khoa học: Transcriptional regulation of the desferrioxamine gene cluster of Streptomyces coelicolor is mediated by binding of DmdR1 to an iron box in the promoter of the desA gene doc

... box), explaining the regulation of desAexpression by binding of DmdR1 to the iron box. The same overlapping was found in S. pilosus.DmdR1 binds to the promoter region of desABCD in both S. ... role of iron and the DmdR1 regu-lator in the transcriptional control of the desferrioxam-ine cluster. In this study, we report the deletion of the ﬁrst gene of the desABCD gene cluster (desA)inS. ... for the treatment of iron overload duringmetabolic alterations in humans. Initial work on the biosynthesis of desferrioxamine B in S. pilosus indica-ted that the ﬁrst step in the desferrioxamine...

Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot

... regulated independently by the main fuelsources in muscle and by insulin through directlyaltering the expression of the human PDK2 and PDK4isoforms. In addition to our ﬁndings, insulin has been ... regulation of mRNA and protein expression, anincrease in PDK4 protein abundance independent of ABCDFig. 2. Semi-quantitative RT-PCR showing the effects of insulin and LY294002, wortmannin, ... expression of these isoforms. The regulatory in uence of glucose, fatty acids and insulin on PDK2 and PDK4 mRNA expressionWe examined the effects of the two predominant meta-bolic fuels in muscle,...

Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot

... loop of the protein that might b e involved in electrostatic binding of the natural electron donor ferredoxin (M. Winkler,B. Neil & T. Happe, unpublished results). In the N-terminus of other ... that the proteins containing two [4Fe–4S] clusters a re ancestors of the Fe-hydrogenases [55]. The N-terminus of the C. reinhardtii and S. obliquus proteins is strongly reduced, and conserved cysteines ... Anaerobic treatment of maize seedlings alters the proﬁle of total protein synthesis[52,53]. It is known that the induction of the anaerobic proteins is the result of a n increased mRNA level....

Báo cáo khoa học: Expression analysis of the nucleocytoplasmic lectin ‘Orysata’ from rice in Pichia pastoris ppt

... calculated using the forceﬁeld of discover3 and used to anchor the pyranose ring of the sugars into the binding sites of the lectin. The position of mannoseobserved in the Man–banana lectin complex ... energyover the threshold value. The docking of MeMan into the carbohydrate-bindingsites of Orysata and other JRLs was performed with the program insightii. The lowest apparent binding energy(Ebindexpressed ... resulted in a subdivision of this family into two groups: the galac-tose-binding and the mannose-binding lectins. In con-trast to the galactose-binding JRLs that aresynthesized on the endoplasmic...

Báo cáo khoa học: Functional expression of the quinoline 2-oxidoreductase genes (qorMSL) in Pseudomonas putida KT2440 pUF1 and in P. putida 86-1 Dqor pUF1 and analysis of the Qor proteins doc

... plots.Determination of the metal contents of the Qor proteins, and detection of the nucleotide moiety of the molybdenum cofactor The contents of molybdenum and iron were determined byinductively ... from quinoline [1,2]. Besides quino-line, some quinoline derivatives and the benzodiazinesquinazoline and quinoxaline are accepted as substrates [1,3].Like other enzymes catalysing the hydroxylation ... the presence of CMP and AMP (from FAD). GMP was not present in anyQor extract, indicating that the host strains did notincorporate Mo-MGD, or free GMP, into the cofactorbinding domain of the...

Báo cáo khoa học: Thermodynamic analysis of the unfolding and stability of the dimeric DNA-binding protein HU from the hyperthermophilic eubacterium Thermotoga maritima and its E34D mutant pdf

... Unfolding and stability of Thermotoga maritima HU (Eur. J. Biochem. 271) 1507Thermodynamic analysis of the unfolding and stability of the dimericDNA-binding protein HU from the hyperthermophilic ... multiple24-curve fitting of DSC data to the two-state model. The dashed linesshow the confidence interval of the fitting. The results of the individ-ual curve fittings (DHU,mvs. Tm) for both the wild-type ... the reversibility of unfolding and avoid postunfolding aggregation. Judging by the complete reproducibility of the calorimetric traces aftercooling the sample within the DSC cell, the heat-inducedunfolding...

Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx

... number of repeateddomains; six in the case of gelsolin itself, adseverin and villin, and three in capG, fragmin and severin (for a review,see [2]). The binding of calcium to gelsolin and to actinbound ... to G2 domain.Footprint of G2 on gelsolin G4–6 domainTwo sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA (Fig. 3). The interaction of these two sites ... for the various Calcium-binding sites so that IG1 is the type I binding site within G2 and IIG6 is the type II binding site within G6. Type I binding sites are coordinatedby gelsolin and actin...

Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx

... protec-tive roles of spermine in free radical scavenging and DNA shielding, and resulting in an overall increased potential for oxidative DNA damage in these cells. The results of these studies therefore ... components of the FAD-bindingdomain, and are therefore essential for catalysis[28,33–35].Kinetic properties of SMO5Polyamine oxidase activity of SMO5 and SMO ⁄PAOh1 was measured using increasing ... resulting in the pro-duction of several catalytically inactive SMO proteins [32], including the PAOh4 protein utilized in the pres-ent study. Although many isoforms have been identi-ﬁed for the...

Báo cáo khoa học: "Transparent combination of rule-based and data-driven approaches in a speech understanding architecture" pot

... transcribed and an-notated the data using a simple Java-based tool,randomly selecting 75% of it for use in training and keeping the rest for testing. During the course of the project, we routinely ... follows:3.1 Training and decodingTraining data for ALTERF is supplied in the form of a text file containing one example per line, in the format1. Initialise R(u) to the empty set.2.Use the feature ... features.•Quantity of training data.•Modality (text or speech) of training data.We randomly divided the training corpus intoten equal pieces, and trained on subsets rangingfrom 10% of the corpus to the...

Báo cáo khoa học: Making sense of G-quadruplex and i-motif functions in oncogene promoters pot

... accessory protein or aminor recombinant protein [34]. Studies show that anR88A mutation (arginine to alanine) in the nucleotide-binding site eliminates binding of the NM23-H2 tosingle-stranded DNA. ... IINM23-H25′3′3′5′OFFABCED5′3′3′5′OFFFig. 5. Cartoon showing the involvement of NM23-H2, nucleolin and a G-quadruplex-interactive compound in modulating the activation and silencing of the NHE III1 in ... (G-rich strand) and i-motif (C-richstrand) folding patterns and structures in the promoterregions of oncogenes. Accompanying minireviews in thisissue discuss other aspects of the biology of G-quadru-plexes...

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