Báo cáo khoa học: The role of residue Thr249 in modulating the catalytic efﬁciency and substrate speciﬁcity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 ppt

... compilation ª 2006 FEBS The role of residue Thr249 in modulating the catalytic efﬁciency and substrate speciﬁcity of catechol-2,3-dioxygenase from Pseudomonas stutzeri OX1 Loredana Siani1,*, Ambra ... two residues and the edge of the substrate ring could prevent bind-ing of 3,6-DMC and 3,5-DMC, as shown in Fig. 2-D,E,F. Thus, the binding of 3,5-DMC and 3,6-DMCto the active site of C2,3O could ... differentsubstrates. The models of the complexes indicate that the orien-tation of the substrate in the active site pocket of the C2,3O is very similar to that observed in the structure of the DHBD and...

Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt

... Tissue-specificexpression of mast cell granule serine proteinases and their role in inflammation in the lung and gut. Immunol-ogy 105, 375–390.5 Caughey G (2001) New developments in the genetics and activation of ... on serglycin. Together,these findings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting into the constitutive path-way of secretion, ... immu-nohistochemistry, and were released following exposure to calcium iono-phore, indicating that they were correctly targeted into secretory granuleseven in the absence of serglycin. In the absence of serglycin,...

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... in obese mice In order to gain insights into the potential biologicallyrelevant role of miR-27 in the regulation of adipose tis-sue functions in vivo, we examined the expression of miR-27 in ... together, these results illustratea critical and speciﬁc role of miR-27 in the regulation of adipogenic differentiation.miR-27 prevents the induction of PPARc and C⁄EBPa In order to delineate ... differentiationwas initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were ﬁxed on day 4 of differentiation and stained with hematoxylin and eosin....

Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx

... withglycine alone was performed and no binding was observed. In our hands, as others [54], the most reproducible datawas obtained from injections of the metal into a solution of the protein. Typically ... proteins they probably formpart of the copper binding sites in this protein.Although there are four histidines in the C-terminal,another histidine is present at position 18, and given the folded ... research is thatunderstanding what causes the loss of appropriatemetal binding might be crucial for the understanding of the role of amyloidogenic proteins in a number of neurodegenerative disorders.Experimental...

Tài liệu Báo cáo khoa học: A role for the intersubunit disulﬁdes of seminal RNase in the mechanism of its antitumor action docx

... mechanism of antitumouraction of BS-RNase is based on the ability of the proteinto resist the neutralizing action of the cytosolic RNaseinhibitor, a resistance due to the dimeric structure of the enzyme. ... devoid of toxicity as native, dimericBS-RNase (data not shown).It is noteworthy that in the inactive MCM, MCA and MAE monomers Cys31 and Cys32, the cysteine residuesoriginally involved in the intersubunit ... hypothesize that the cytotoxicactivity of the latter monomers was due to the presence in these proteins of disulﬁde bonds, with their potentialchemical instability. It is well known that in the...

Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

... of b-strand 3Õ (i.e. the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop connecting b-strand and a-helix and contiguous residues on the a-helix and the last part of b-strand ... in which the sequence of the b-strands was maintained but the amino acids from loop 3 had been replaced bymultiple Ser and Gly residues. Therefore, in the sequence of the whole b-hairpin structure of ... action of defensins. However, our resultscould be compared with results obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... deviates from the original position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain at residue 169 (Table 1 and Fig. ... accessibility of His210. The size of the side-chain at residue 169 and the pK2showed a clear linear relationship (Fig. 3B). The pK2increased as the size of the residue at position 169 increased. The ... effect of the size of the residue at position 169 may berelated to the solvent accessibility of the side-chain of His210 (Fig. 3C). When the accessibility of the side-chain of His210 increased, the...

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... inhi-bitor of growth protein 3 (ING3) PHD, which isanother domain involved in chromatin binding and clearly more divergent (e-value 0.019) from DIDO and the other sequences. As the alignment ... distant from the other bran-ches, which was indeed predicted. The other maingroup, obtained 97% of the time in 20 740 exploredtrees, contained the DIDO PHD sequences clusteredwith the PHF and the ... the context of DNA binding and chromatin regulation. These homologues includemembers of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated CpGislands [7], which are...

Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

... pregnenolone, including the effect of the steroid products on the yeast sterol synthesispathway, acetylation of the product, availability of the substrate, electron flow and localization of the differentprotein ... steroid-producing cells withrespect to the coupling of the SCC and 3b-HSD reactions; the inhibitory effect of steroid products on CYP11A1 substrate biosynthesis; the regulation of intracellular ... increase the cycling of sterols by esterification and hydrolysis, resulting in anincrease in intracellular sterol trafficking. It is unclear, then,whether the corresponding increase in pregnenolone...

Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

... show the critical role of the lastamino acids in the C-terminus of MaL. Deletion of the four last amino acids strongly reduced the expression of the protein in T. reesei and S. cerevisiae. Further-more, ... C-terminal carboxylate group in MaL ishydrogen-bonded to the side chain of His140, whichis one of the His residues coordinating the T2 copper in the trinuclear center.C-terminal sequencing of the ... expressedboth in yeast and in T. reesei, was practically inactive.Also, the kinetic parameters were altered. The change of only one amino acid in the C-terminus of the lac-case, giving the Tr(L559G) and...

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