Báo cáo khoa học: Slow conformational dynamics of the guanine nucleotide-binding protein Ras complexed with the GTP analogue GTPcS pot

... between the correspondingT2relaxation times for the conformational states 1a and1b of Ras( T35S)•Mg2+ GTPcS. Complex of Ras Mg2+ GTPcS with the Ras- binding domain of Raf-kinaseAddition of the ... al. Conformational dynamics of Ras bound to GTPcS FEBS Journal 274 (2007) 1419–1433 ª 2007 The Authors Journal compilation ª 2007 FEBS 1421 Conformational states of Ras complexed with Mg2+ GTPcS Figure ... on Ras( wt)•Mg2+ GTPcS (data notshown). Binding of GTPcS to the Ras protein leads torather large chemical shift changes. In contrast to the observations made for the GTP analogues GppNHpand...

Tài liệu Báo cáo khoa học: Cosubstrate-induced dynamics of D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida ppt

... A˚2higher than the average B factor of the subunit. In the subunits with bound NAD+, the B factor of the two residues iscomparable with the average B value of the subunit.Nevertheless, the conformation ... [9]. With the exception of Gln193, theseresidues are located in the deep active-site cleft. The exact boundaries of the ﬂexible loop differ amongenzymes. Based on the structure of PpHBDH, the loop ... pocket of PpHBDH are very simi-lar to those of PfHBDH, with a distance of about14 A˚between the C2 of nicotinamide and the C6 of the adenine ring. As indicated in the superpositions of Figs...

Tài liệu Báo cáo khoa học: Glycomics-based analysis of chicken red blood cells provides insight into the selectivity of the viral agglutination assay docx

... treatment of the collected pools to determine the distribution of sialicacid. Next, we completed online MS ⁄ MS analysis of the major species; some of these species were furtheranalyzed by TOF ⁄ TOF ... cRBCs. Deﬁning the glycanspresent on the surface of cRBCs will allow either for the design of strategies to optimize the agglutination assayor the design of alternative strategies for the detectionand ... analysis of the sample after enzymatic treatment with sialidaseS showed that the retention time of some of the peaksremained the same, indicating no sialic acid cleavage,whereas there was the appearance...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... implications of the observed VpUcytstructureOne of the most studied activities of VpU is the induc-tion of CD4 degradation in the endoplasmatic reticu-lum of infected CD4+cells. Only the cytoplasmicdomain ... calculation of preliminary protein structures [79]. radar runs in an iterative manner. The input of the ﬁrst cycle consisted of the 3D NOESYdata, the amino acid sequence of VpUcyt and the chemicalshift ... isthus often called accessory protein. The most well-deﬁned function of VpU is downregu-lation of CD4 in the endoplasmic reticulum, which ismediated by the cytoplasmic region of the protein...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... considering the oxidation of the substrate byT. reesei TYR2. The closer to the hydroxyl group of phenol the amino group was, the slower was the oxida-tion of the substrate. For most of the substrates ... the C-termi-nus of the protein, the most C-terminal trypticpeptide, as compared with the theoretical tryptic pep-tide map of the deduced protein, was found to beSQAQIK (m ¼ 673.376 Da). The ... of the sequence. Together with the results from the glyco-peptide analysis (see below), the peptide analyses com-pletely conﬁrmed the deduced amino acid sequence of the secreted protein. The...

Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf

... Equalmobilities of the radioactive and the protein- containingbands indicated covalent attachment of DNA to the enzyme. The yield of the photochemical cross-linkingreaction was calculated as the ratio of the ... introduction of the TFMDPh group in close proximity to the oxoG residuehas no effect on the recognition and binding of DNAduplexes by the Fpg protein. Speciﬁcity of Fpg protein binding The interaction ... oxoG and 5¢ adjacent to the oxoG-pairedcytidine residue, with amino acid residues of E. coli Fpg protein. These results, together with data from ongoingstudies of the Fpg protein and its pro-...

Tài liệu Báo cáo khoa học: Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc

... bondbetween the hydrogen of OH9 (HO9) and the oxygen of OH7 (OH7) which can contribute to the stabilization of the C7–C9 region of the macrolide.Structural analysis of nystatin A1 The spectral ... along the longaxis of the polyene with the D-mycosaminefront (indicated with an arrow). The bonds of the hydroxyl groups are represented with boldlines. Carbons are labelled according to theirnumbering ... However, for the C1–C7 segment, the two hydroxyl groups on C3 and C5 are, respectively,hydrogen bonded with the CO groups of C1 and C7 in the majority of the models. For the C10–C13 segment, the hydroxyl...

Báo cáo khoa học: Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebraﬁsh (Danio rerio) pptx

... shows the distribution of fapb6 transcripts in the distalregion of the intestine (A), liver (B), ovary (C) and the adrenal homo-log of the ﬁsh kidney (D). Panel 2 shows the locations of the distalregion ... localize the rat Fabp6 protein andin situ hybridization to localize Fabp6 transcripts to the enterocytes of the ileum, luteal cells of the ovaryand a subpopulation of steroid endocrine cells of the adrenal ... acids, respectively. The intron ⁄ exon structure of the fabp6 gene from zebraﬁsh is consistent with that of all other fabp genes studied to date [1], with the excep-tion of the muscle-type FABP...

Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

... inde-pendent of the origin of the cloned cDNA and whether the enzymes are naturally located in mitochondria.However, the data do not demonstrate the proper tar-geting of the proteins to mitochondria. Therefore,ultrastructural ... assembly of the iron–sulfur units.It is of interest therefore to know whether the fold-ing of these two proteins around their iron–sulfur clus-ter occurs similarly in mitochondria and in the cytosol.To ... DNAstability and subunits of the KGDH complex were alsobound to these large structures, together with > 20other mitochondrial proteins [22]. However, the com-position of the aggregates in this...

Báo cáo khoa học: Structure ⁄function analysis of spinalin, a spine protein of Hydra nematocysts doc

... at the acidic C-terminus of the protein is not occupied. MS of the nonreduced protein showed a mass of 23 700.0 Da. The difference between the molecularmasses of the reduced and nonreduced protein ... analysis of the noncross-linked spinalin of the ﬁrst peak, only monomers arepresent (Fig. 4A). The size of the particles is in agree-ment with this prediction, suggesting that the aggrega-tion of ... consistent with the molecular mass of the dimeric protein. Reduction and heat denatura-tion converted parts of the dimeric spinalin to mono-meric proteins with an apparent molecular mass of  26...

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