Báo cáo khoa học: Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content potx
Báo cáo khoa học: Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content potx
... Thermal stability of homologous functional units of Helix pomatia hemocyanin does not correlate with carbohydrate content Betu¨l T. Yesilyurt1, Constant ... 2008)doi:10.1111/j.1742-4658.2008.06507.xThe thermal stability of the eight functional units of b -hemocyanin of thegastropodan mollusc Helix pomatia was investigated by FTIR spectros-copy. Molluscan hemocyanin functional units have ... the functional units of H. pomatia b -hemocyanin, designated HpH-a to HpH-h, differ considerablyin their carbohydrate content (0–18 wt%). Most functional units are excep-tionally stable with...
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Báo cáo khoa học: Dynamics, stability and iron-binding activity of frataxin clinical mutants pdf
... material is available as part of the online articlefrom http://www.blackwell-synergy.comPlease note: Wiley-Blackwell are not responsible forthe content or functionality of any supplementarymaterials ... of patients characterized.In preliminary studies, we addressed the question of whether prevalent mutations that result in classicalFRDA phenotypes were correlated with completeimpairment of ... and animpairment of the biosynthesis of iron-sulfur proteins[1–4]. The majority of FRDA patients (> 95%) arehomozygous for a GAA repeat expansion within thefirst intron of the frataxin gene...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... T (2005) Functional importance of Ca2+-deficient N-terminallobe of molluscan troponin C in troponin regulation.Arch Biochem Biophys 436, 83–90.H. Tanaka et al. Functional regions of molluskan ... (1971) The regulation of rabbitskeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin–troponin complex with actin and the proteolytic fragments of myosin.J Biol ... 375–380. Functional regions of molluskan TnI H. Tanaka et al.4486 FEBS Journal 272 (2005) 4475–4486 ª 2005 FEBSComparative studies on the functional roles of N- andC-terminal regions of molluskan...
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Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx
... comparison of Tm5a with smTm allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscleexon 1b, and comparison of Tm5a with Tm5b allowsthe effects of exchange of exon 6 ... presence of exon 1b dramatically decreases the measurablecalorimetric enthalpy of the thermal unfolding of Tm observed with DSC,although it has no influence on the a -helix content of Tm or on ... enthalpy of free Tm (DH3) with the enthalpy of peak 1 subtracted (DH3) DH1)(Table 2). (It is noteworthy that in the absence of actinthe enthalpy of reversible unfolding of Tm did not sig-nificantly...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... between 300 and 550 nm with a scanning speed of 150 nmÆs)1and an excitation slit of 5.0 nm.Calorimetric measurements Thermal analysis of protein denaturation was performed with DSC (a model 6100 ... terms of assembly of fragments is discussed.ResultsCD spectraSecondary structure of SNase and its mutants was deter-mined by CD with spectrapolorimetry measurements.The approximate fraction of ... thesecondary structure of the protein disintegrated. Usingthe peak at 222 nm of the wild-type protein as an index of helical conformational stability, this CD spectrum of W140O suggests a decrease...
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Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... provide information Helix H Helix G Helix EHelix D Helix A Helix BHeme Helix C Helix FCCDBGHFNAEFig. 1. Three-dimensional structure (top) and amino acid sequence(bottom) of human Ngb. ... thermophilicAquifex aeolicus [54]. Functional stability of NgbThe conformational stability of the apoNgb structurein acid is in agreement with the enhanced functional acid stability of the holoprotein. In ... estimate of the stability of the Ngb fold at acidicpH was obtained by measuring the urea-mediatedFig. 4. Proteolysis of apoNgb and apoMb with pepsin. Proteolysis of the proteins by pepsin (E ⁄ S of...
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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
... thanBsCsp (TM of 50 °C, pH 7; DGU of 11 kJÆmol)1,pH7,20 °C) [44]. This difference in thermodynamic stability correlates with two orders of magnitude slower unfold-ing of TmCsp as compared ... [15]. Analysis of a structural model of AmFd suggests that a combination of additional sur-face ion pairs, the zinc cofactor, and an efficientlypacked core govern the high stability of this protein[36]. ... Extrapolation of kineticdata in the presence of denaturants suggests thatunfolding of the hyperthermostable Fds at pH 7 inbuffer (20 °C) requires hundreds of years [35]. For the homologous [2Fe)2S]...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... whilemost of the mutations of negatively charged glutamicacid or aspartic acid residues to uncharged glycine resi-dues do not disrupt the secondary structure of SNase,replacement of E129 with G ... pro-tein secondary structure. However, this replacement of G at E129 does not significantly influence its wholeprotein stability (see Results of thermal analysis sec-tion below).Tryptophan fluorescence ... interactions of G75 with H121 and K9, and of G129 with K110 and K133. Other chargedamino acids such as D73, D83 and E101 reinforce the interactions of E75 and E129.H M. Chen et al. Local stability...
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Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx
... of D276 and N305 in rat HDC) arepredicted to interact with the imidazole ring and thephosphate group of PLP, respectively [18,36,37]. It isnoteworthy that in spite of the high flexibility of ... inhibitor was not ableto protect the enzyme against thermal denaturation.The increased resistance of the a-FMH-treated protein to thermal denaturation would indicate that the covalentbinding of the ... adduct within the catalytic centerin addition to those established by the internal aldiminealone. From the shape of the a-FMH-treated proteinspectra, stabilization of some a -helix by the cofactor...
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Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx
... 8.5, we studied thermal stability of 17b-HSDcl from CD and UV meltingcurves, from which the temperature of denaturation(Td) and the van’t Hoff enthalpy of denaturation(DH°vH )of1 7b-HSDcl were ... pH > 10. Of note, the observed thermody-namic stability of 17b-HSDcl at 25 °C, with a value of 15.3 kJÆmol)1(0.06 kJÆmol)1amino acid), is muchlower than for the majority of globular ... concentrations of 1.2and 0.4 m, respectively. Binding of the coenzyme NADPH to 17b-HSDclcauses local structural changes that do not significantly affect the thermal stability of this protein.AbbreviationsCd,...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui rochuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ