Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot

... synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex Monica Averna, Roberto Stifanese, Roberta De Tullio, Franca Salamino, Sandro ... approximately 80 90% of eNOS protein and A BFig. 2. Levels of calpain isoforms and calpain substrates in the aorta of NMS and HMS rats treated with HSD. Aliquots (100 lg protein) of brain soluble material ... produced by active calpain [32], theseobservations further indicate that calpain is activated in both tissues, although at a higher rate in aorta. Fur-ther direct evidence in support of calpain activation...

Báo cáo khoa học: In vivo production of catalase containing haem analogues pot

... produced, isolated and analysed novel catalasevariants in which the iron atom of the haem prosthetic group is replaced by other metals, i.e. cobalt, gallium, tin, and zinc, and also variants con-taining ... case of Ga-PP-KatA, an additional protein band of about 110 kDa was observed. This band cor-responds to KatA dimer, as determined by immuno-blot analysis (Fig. 1, lane I).Amino acid analysis ... Lanes A and N each contained80 ng of puriﬁed haem-containing E. faecalis catalase (Fe-PP-KatA).Lanes B–M each contained 2 lg of total cytoplasmic protein. Fig. 2. SDS ⁄ PAGE of preparations of isolated...

Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deﬁciency and hypervitaminosis pot

... Medicina y Farmacia., Universidad de Valencia, Valencia, SpainSeveral clinical trials have revealed that individuals who weregiven b)carotene and vitamin A did not have a reduced risk of cancer ... in controlling the expression of p53 and related genes that areessential for maintaining the integrity of tissues.Materials and methodsRatsWistar rats (Charles River Lab., Barcelona, Spain)8,9,10weregiven ... search analysis of the EMBL [19] and GenBank[20] databases were performed using the BLASTprogram [21].RNA isolation and Northern blot analysisTotal RNA from the different tissues used was isolated...

Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starﬁsh sperm and its induction by egg jelly triggering the acrosome reaction pdf

... firstdemonstration of an in vivo activation of transglutaminaseleading to the formation of a cross-link in intracellularproteins.Keywords: starfish; sperm; transglutaminase; chromatin;acrosome reaction. The ... the acrosome reactionKazuto Nunomura*, Satoru Kawakami†, Takahiko Shimizu†, Tomohiro Hara, Kazuhiro Nakamura,Yudai Terakawa, Akiko Yamasaki and Susumu Ikegami‡Department of Applied Biochemistry, ... through the acrosomereaction-induced activation of nuclear transglutaminase by an increase in the intracellular concentration of Ca2+.Toour knowledge, this is the first demonstration of intracellulartransglutaminase-catalyzed...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... containing 1) [9]. The KIND domain in theseproteins is localized to the N-terminal region, and theirspeciﬁc functional domains are located in the C-termi-nal region. The C-lobe of protein kinases ... the protein protein interactionbetween v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding coremodule in MAP2) across a range of amino acid resi-dues, and provided ... indicated that the RasGEF activity of v-KIND induces the phosphoryla-tion of MAP2 by JNK1 and ⁄ or ERK via the activation of the Ras–Raf–MAP kinase pathway [12]. These resultsappear to be in...

Báo cáo khoa học: In vivo RNA interference in oyster – vasa silencing inhibits germ cell development pptx

... sampled. Their gonads wereimmediately dissected: a large transverse section of all the gonadic area was taken for histological examination, and the rest of the gonad was placed in total RNA and ... (lane 7). Twelvemicrograms of total protein extract from each tissue was loadedinto the gel. A single band of about 79 kDa was detected in female and male gonads.DigestiveglandGonadMantleOoFig. ... tons; [1]), it plays a sentinel role in estuarine and coastal marine habitats [2], and itbelongs to the Lophotrochozoa, a vast and diversebranch of bilaterian animals that have been little stud-ied...

Báo cáo khoa học: In vitro and in vivo self-cleavage of Streptococcus pneumoniae signal peptidase I pot

... peptide. This signal sequence is involved in guiding the protein into the targeting and translocatingpathway by interacting with the membrane and othercomponents of the cellular secretory machinery ... the membranewere excised and the N-terminal amino acid sequence of each protein was determined by automated Edman degradation. Cleavage of prestreptokinase by S. pneumoniaeSPase I and SPase37–204We ... and the cells maintain the active SPase I at the highest level in the exponential growth phase.MATERIALS AND METHODSMaterials and bacterial strainsRestriction enzymes, T4 DNA ligase and Taq...

Báo cáo khoa học: Hodgkin Reed–Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo Novel insight into the inﬂammatory features of classical Hodgkin lymphoma ppt

... positivemode. The spray voltage was set to 4500 V, the capillarytemperature was 375 °C and sheath and auxiliary gas wasoptimal at 60 and 5 arbitrary units, respectively. Skimmeroffset was at 10 V and ... type 1 and 2 [6,8]. Ithas been shown that patients with asthma and airway in ammation have increased expression of 15-LO-1 protein and increased activity of 15-LO-1 in the lungcompared to healthy ... permeability of the endothelial cell monolayer in vitro, indicatingthat they can modulate and enhance vascular perme-ability, a hallmark of in ammation [11]. It has beenknown for many years that 15-LO-1...

Tài liệu Báo cáo khoa học: Inﬂuence of modulated structural dynamics on the kinetics of a-chymotrypsin catalysis Insights through chemical glycosylation, molecular dynamics and domain motion analysis pptx

... in these parameters. Analysis of the changes in internalhydrogen bond composition for the protein- glycan con-jugates indicates that for all of the conjugates there wasalso an increase in these ... of the protein decreases atincreasing glycosylation levels. This is in accord with the increased thermodynamic stability exhibited by natural glycoproteins [34,56–59] and also with dataobtained ... A 2, and A 3are the fractions of the fast, slow and stable amide protons and kHX,1 and kHX,2are the apparentexchange rate constants for the fast and slow amide pro-tons. Results were interpreted...

Báo cáo khoa học: Inﬂuence of inﬂammation-related changes on conformational characteristics of HLA-B27 subtypes as detected by IR spectroscopy potx

... by X-ray crystal-lography in these structures is a string of water mole-cules between the a- helix and the major b-sheet thatare distinctly located in the wild-type protein and in the variants. ... ornonself proteins are usually 8–12 amino acids long and are accommodated in a binding groove of the molecule by means of HLA allele-characteristic ‘anchor’ aminoacids. This selectivity of MHC ... deuterated state) to 100%. This approach is only an approximation because: (a) the amide IIbands of different conformations of proteins havedifferent peak maxima, and (b) IR bands due to aminoacid...

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