Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... FEBS 2481 Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions Janne Molnes1,2,3, ... E256 and E290) and the S-domain(residues T168 and K169) (Fig. 1B). To identify the contact residues involved in the initial binding of Glc to the super-open state (Fig. 1C) of this two domainhinge-bending ... induced by Glc binding; (b) to identify the activesite residues involved in the binding of Glc to the super-open state (Fig. 1C) of this two domain [large(L) and small (S)] enzyme, and thus the...
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Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
... on the surface of the hTNF-a protein,enabling easy access to catalytic antibodies. The acces-sibility of the TNF-a molecule to ETNF-6-H to is one of the most important factors in cleaving the ... chainscapable of the degradation of urease in Helicobact-er pylori. The catalytic antibody light chain, UA15-L,could suppress the number of bacteria infecting the mouse stomach. In these catalytic ... digested by the catalytic reac-tion of ETNF-6-H. To determine whether ETNF-6-Hcan digest intact hTNF-a, 12% gel SDS ⁄ PAGE withsilver staining was performed to monitor the timecourse of the cleavage...
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Báo cáo khoa học: Catalytic mechanism of the primary human prostaglandin F2asynthase, aldo-keto reductase 1B1 – prostaglandin D2 synthase activity in the absence of NADP(H) pptx
... & Penning TM (1996) Character-ization of the substrate binding site in rat liver 3alpha-hydroxysteroid ⁄ dihydrodiol dehydrogenase. The roles of tryptophans in ligand binding and protein fluores-cence. ... core in this family [ 3–6 ]. To iden-tify the catalytic residues involved in the PGDS and PGFS activity catalyzed by AKR1B1, we introducedsite-directed mutagenesis into the tetrad, generating the D43N, ... demonstrated that the substrate PGH2was bound to the substrate- binding cavity in anextended conformation at the top of the (a ⁄ b)8-barrel(Fig. 4A,B). The docking calculation, including molec-ular...
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Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf
... in catalysis and the specific involvement of the catalytic residues in the enzymatic reaction. The two zinc ions in the active metal center are thought to participate in substrate binding by activating the ... the deprotonation of the zinc-bound water to form the nucleophilic hydroxide and less involved in substrate binding; and (c) the two zinc ions in the active site par-ticipate in stabilizing the hydroxide ... nucleophile. The binding mode of the water⁄hydroxide to the di-zinc centerInhibition of SGAP by fluoride anions was utilized to assess the binding of the water ⁄ hydroxide to the activemetal...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx
... leading to autophosphoryla-tion of tyrosine residues on the cytoplasmic domain of the receptor, thereby creating docking sites for adaptor pro-teins, such as Shc and G rb2. The latter protein ... again after returning to a low level. On the other hand, the s ame phosphorylation of EGFR by PKC isknown to cause decreased EGFR tyrosine kinase activity,its binding affinity for EGF [3 0–3 2] and ... w as not the case in the absence of P MA(Fig. 3). Furthermore, the ratio betw een the ERK-PPconcentration after stimulation with EGF and PMAtogether on the one hand, and the sum of the ERK-PPconcentrations...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... receptor Toll and thereby initiates a cyto-plasmic signaling pathway, resulting in the release of arel family transcription factor Dorsal from the inhibi-tor protein Cactus to activate genes involved ... perform immune functions. Thismechanism leads to activation of phenoloxidase, whichoxidizes catechols, leading to the formation of toxicquinones and melanin [1,2], and to the activation of cytokines ... reported in other genes, such as human and Drosophila Gsa (the a-subunit of the guanine nucleotide -binding protein)isoforms [41,42].ProSpa¨tzle-1 mRNA was detected in hemocytes and fat bodies of...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... characteristic of the fasting state. The inclusion of fatty acids resulted in increased activities of the a-1 or the a-2 isoforms of AMP-activated protein kinase (AMPK), increased phosphoryla-tion of acetyl-CoA ... mechanism. The finding that epinephrine totally blocked the activation of AMPK by palmitate is of note and requiresfurther investigation of the adrenergic signalling mechanismthat is involved. ... frombeing blocked by the phosphatidylinositol 3-kinase inhibitorwortmannin, no other details of this insulin process areknown. A novel and physiologically interesting observation of the present...
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Báo cáo khoa học: Neuropeptide Y, B-type natriuretic peptide, substance P and peptide YY are novel substrates of fibroblast activation protein-a pdf
... throughout the brain, and is involved in the regulation of energybalance by stimulating increased food intake. Pep-tide YY (PYY) is produced by the gastrointestinaltract, and, via NPY receptor binding, ... of natural substrates is poorly characterized.Identifying substrates is a crucial step in gaininginsights into the precise functions of proteases and their mechanisms of action in biology and ... relative abilities of recombinant human FAP to catalyse the degradation of known DPP4 substrates of the gastrointestinal hor-mone, neuropeptide and chemokine classes by MALDI-TOF MS analysis.ResultsEnzyme...
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Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf
... responses, the significance of ERK signaling in terms of regulatingmiRNA expression is of great interest. To study the role of the ERK1 ⁄ 2 pathway in the regulation of miRNA expression, we first determined the ... ERK1 ⁄ 2 activation (Fig. 4B). Wealso confirmed inhibition of protein synthesis by monitoring expression of c-Fos protein, which is aFig. 2. Activation of ERK1 ⁄ 2 pathway is involved in miR-221 ... Muta-tions introduced into the predicted binding sitealmost eliminated this suppression. These results sug-gest a direct interaction of these miRNAs with the predicted target site of the Bim 3¢...
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Báo cáo khoa học: Catalytic residues Lys197 and Arg199 ofBacillus subtilis phosphoribosyl diphosphate synthase Alanine-scanning mutagenesis of the flexible catalytic loop ppt
... respect to ATP and noncom-petitive inhibitors with respect to Rib5P. These resultsindicate that binding of the substrates is ordered withinitial binding of ATP followed by binding of Rib5P. In ... ordered binding of the substrates with ATP binding first and a random release of the products[16].Discussion The kinetic analysis of the K197A and R199A mutantPRibPP synthases indicates that these ... seems to indicate aninvolvement of these residues in the binding of the substrate ATP. The second type, represented by R198A, P200A, V204A, and V207A, had reduced Vappvalues compared to the...
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