... al. Role of Glu53 and Asp2 87 in the stability in 7. 9 M GdmCl
FEBS Journal 274 (20 0 7) 61 67 6 1 79 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 6 1 79
Tertiary structure in 7. 9 M guanidinium chloride
)
the ... Stern–Vollmer plots for the
proteins in the native state were 7. 9 m
)1
, 8.6 m
)1
,
8.4 m
)1
and...
... 191 2– 192 4.
45 Maathuis FJMM & Sanders D ( 199 4) Mechanism of
high-affinity potassium uptake in roots of Arabidopsis
thaliana. Proc Natl Acad Sci USA 91 , 9 272 9 276 .
46 Maathuis FJMM & Sanders ... (MS) plates and in a medium containing 100 l
M K
+
(LK). Each data point was repeated three
times. (B) FW of the 7- day-old wild-type, helps mutant and OE seed...
... the other strains only one determination was made. Strains mf1 and
mf2 are unable to grow in minimum medium. ND, not determined.
Lipid
Strain
WT mf1 p1mf1 p2mf1 p3mf1 p4mf1 p5mf1 p6mf1 mf2 pmf2
TAP ... P16 and P 1 7) or from the minor antenna
complexes (CP26 and CP 29 [ 27] ) accumulated significantly
in the two mutants mf1 and mf2, although some limited
change in the...
... (from herein referred
to as the Stanford parser) was developed and com-
pared to two other systems: MINIPAR, described
in (Lin, 199 8), and the Link parser of (Sleator and
Temperley, 199 3), which ... Length Ori Mean Ori Std.Dev. Inh Mean Inh Std.Dev. Count
3-6 96 .61 7. 42 90 .34 15.20 56
7- 8 92 . 37 15 .77 86.00 19. 34 57
9- 10 92 .80 8.18 82 .73 17. 15 45
11-20...
... developed by Kimmo Koskenniemi
( 198 3), henceforth KIMMO, has spawned
much subsequent research in the same
framework (Karttunen, 198 3; inter alia).
Important design features of this model
include ...
content (McCarthy, 1 97 9 ; Levin, 198 5).
Work on reduplication I by Marantz ( 198 2) and
by Levin ( 198 5) has argued convincingly that
reduplication involves the...
... h in the medium without serum. Subsequently,
10 lgÆmL
)1
of recombinant cartducin was added to the
medium for 5, 15, 30, and 60 min. In another set of
experiments, cells were treated with an increasing ... 10%
fetal bovine serum (FBS; PAA Laboratories, Linz, Aus-
tria), 2 mml-glutamine, and penicillin ⁄ streptomycin at
37 °C in a humidified atmosphere containing 5% CO
2...
... & Struhl K ( 199 0) Folding transition in the
DNA-binding domain of GCN4 on specific binding to
DNA. Nature 3 47, 575 – 578 .
23 Weiss MA ( 199 0) Thermal unfolding studies of a
leucine zipper domain ... Biopolymers
9, 10 59 1 077 .
53 Wiseman T, Williston S, Brandts JF & Lin LN ( 198 9)
Rapid measurement of binding constants and heats of
binding using a new tit...
... PAGE, in the presence or
absence of 5 m
M ATP. Numbers on the top of each lane indicate
the time of digestion in min. (Lower) Densitometric scan showing
the amount of r
32
-C-his (1.2 lM) remaining ... by taking 60 pmol of r
32
in buffer P (50 mm Tris ⁄ acetate, pH 7. 2, 100 mm NaCl,
5mm MgCl
2
,25lm Zn-acetate, 1.4 mm b-mercaptoetha-
nol) containing 5 mm ATP. CIII...
... ( 19 97 ) Nonionic detergents induce
dimerization among members of the Bcl-2 family. J. Biol. Chem.
272 , 138 29 13834.
33. Hsu, Y.T. & Youle, R.J. ( 199 8) Bax in murine thymus is a soluble
monomeric ... lysed with 10 m
M
Tris/HCl (pH 7. 3), 2 m
M
Na
3
VO
4
,0. 4m
M
EDTA, 10 m
M
NaF, 1 m
M
phenyl-
methanesulfonyl fluoride, 2 lgÆmL
)1
aprotinin and leupep-
ti...