Báo cáo khoa học: Probing the determinants of coenzyme speci

Báo cáo khoa học: Probing the determinants of coenzyme speciﬁcity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis potx

... examined the possi- bility of altering coenzyme specificity of the Peptostreptococcus enzyme, and, more specifically, the role of residue 243 and neighbouring residues in coenzyme binding, by introducing ... Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesi...

Ngày tải lên : 30/03/2014, 03:20

8
375
0

Báo cáo khoa học: Probing the determinants of substrate speciﬁcity of a feruloyl esterase, AnFaeA, from Aspergillus niger doc

... chain of the FA. The importance of the interaction between an aromatic tyrosine and the phenolic ring of the substrate is consistent with the biochemical speciﬁcity of this enzyme [4,24]. The ... resulting from the disposition of the equatorial and axial hydroxyls to one side of the pyranose ring of a sugar monomer, aligns itself upon binding with the aro...

Ngày tải lên : 30/03/2014, 20:20

10
382
1

Tài liệu Báo cáo khoa học: Seeking the determinants of the elusive functions of Sco proteins pptx

... transmembrane helix anchoring the protein to the inner membrane of mitochondria [27]. Therefore, the N-ter- minal segment (containing the residues protruding into the mitochondrial matrix, the transmembrane ... function of the CXXXC motif of human Sco proteins could therefore be impli- cated not only in the maturation of the Cu A site of Cox2 but also in the...

Ngày tải lên : 14/02/2014, 18:20

19
743
0

Báo cáo khoa học: Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site pot

... when the crystal structure of the spinach enzyme was determined, ﬁrst in the presence of the inhibitor IpOHA [8] and later with product bound in the active site [9]. There are several interesting ... both valine and leucine. The third branched-chain amino acid, isoleucine, is produced in a pathway that parallels that of valine, employing the same series of enzyme...

Ngày tải lên : 16/03/2014, 18:20

10
339
0

Báo cáo khoa học: Probing the access of protons to the K pathway in the Paracoccus denitriﬁcans cytochrome c oxidase pdf

... given by electrostatic calculations indicating that the redox state of the binuclear center in uences the protonation state of a glutamate residue positioned at the cytoplasmic end of the second ... band width of the contribution at  1718 cm )1 indicates that residues close to the surface, or in the vicinity of several water molecules are involved here. Com...

Ngày tải lên : 30/03/2014, 15:20

9
367
0

Báo cáo khoa học: "Examining the Role of Linguistic Knowledge Sources in the Automatic Identification and Classification of Reviews" doc

... documents and find that the fea- ture vectors corresponding to some of these documents (particularly the short ones) have all zeroes in them. In other words, none of the bigrams from the training set ... available. 612 Finally, previous work has also investigated fea- tures that do not fall into any of the above cate- gories. For instance, instead of representing the...

Ngày tải lên : 17/03/2014, 04:20

8
489
0

Báo cáo khoa học: Structural origins for selectivity and speciﬁcity in an engineered bacterial repressor–inducer pair pdf

... respect to the positioning of the ligands and the conformations of the residues lining the binding site (Table 2). In an initial comparison of the ligand positions of tetracycline, dox and atc in TetR, ... shown in grey and blue). The stereo represen- tation shows that the binding position of the ligands and the orientations of the side chains lining t...

Ngày tải lên : 16/03/2014, 00:20

12
501
0

Tài liệu Báo cáo khoa học: Probing the molecular determinants of aniline dioxygenase substrate speciﬁcity by saturation mutagenesis docx

... was determined on the basis of the possible binding sites identiﬁed by the Site Finder function in moe, as well as the relative position of the indole substrate in the crystal structure of naphthalene ... probe the residues determining the activity as well as the substrate speciﬁcity of AtdA, using molecular modeling and saturation mutagenesis of the...

Ngày tải lên : 19/02/2014, 02:20

12
634
0

Báo cáo khoa học: Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar speciﬁcity in the structural family ppt

... 5 min, the reaction was initiated by the addition of 10 lL of enzyme solution (0.83 mgÆmL )1 ). After 3 min, the reaction was stopped by the addition of 100 lL of concentrated HCl; after mixing, ... contacts with their equiv- alents in the other subunit of the second dimer (Fig. 4A). In the case of P2 1 2 1 2(TcRpiB-R5P), the four molecules in the asymm...

Ngày tải lên : 15/03/2014, 00:20

16
401
0

Báo cáo khoa học: Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190 pot

... The reaction rate was determined at 25 °C by measuring the linear increase in absor- bance at 400 nm during the initial 5 min. Control experiments were carried out in the absence of NAT2. The slope ... centroid of the His107 imidazole ring and the planes of the two ring systems intersect at an angle of $ 30°, Tyr190 and His107 interact by a common aromatic st...

Ngày tải lên : 16/03/2014, 00:20

14
350
0