Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx
Báo cáo khoa học: DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine pptx
... phosphorylates caspase 9 at Thr125. Recombinant His6 caspase 9 (His–C9) or His6 caspase 9( T125A) (both containing the catalytically inactivating C287A mutation)was incubated with recombinant ... of caspase 9 at Thr125 in vitro, these resultsstrongly indicate that DYRK1A targets caspase 9 directly in cells. Harmine is a potent inhibitor of DYRK1A in cells The b-carboline alkaloid harmine ... DYRK inhibitor in vitro by Bainet al. [ 19] . Harmine inhibits purified DYRK1A in thenanomolar range, with DYRK2 and DYRK3 inhibited 10-fold less potently, and little or no inhibition by 1 lm harmine...
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Tài liệu Báo cáo khoa học: A zymogen form of masquerade-like serine proteinase homologue is cleaved during pro-phenoloxidase activation by Ca2+ in coleopteran and Tenebrio molitor larvae docx
... Tokyo).Antibody and immunoblottingAntibody against the Tm-mas was raised by injecting 10 lgof the purified protein into a male albino rabbit withcomplete Freund’s adjuvant and giving a booster injectionof ... b-1,3-glucan for 60 min; lane 2, incubation with b-1,3-glucan only for60 min; lane 3, 4, 5, 6 and 7, incubation with Ca2+only for 10, 20, 30,45, 60 min, respectively; lane 8, 9, 10, 11 and 12, incubation ... containing an approximately 45-kDa protein asdetermined by SDS/PAGE (reducing conditions) werepooled and concentrated by ultrafiltration kit (YM-10membrane). Fractions containing 45-kDa protein...
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Báo cáo khoa học: Enzymatic oxidation of NADP+ to its 4-oxo derivative is a side-reaction displayed only by the adrenodoxin reductase type of ferredoxin-NADP+ reductases potx
... 4003occupancy of the nicotinamide ring of the boundligand in the active site [14]. In this view, it is signifi-cant that the perturbations induced by NADPO bind-ing to FprA were more intense than ... dinucleotide phosphate formation in which the initial and rate-determining step is the nucleophilic attack of thenicotinamide moiety by an active site water molecule. Whereas plant-typeferredoxin reductases ... theenzyme-bound FAD, and is regiospecific in affecting only the C4 of theNADP nicotinamide ring. By protein engineering, we excluded the involve-ment in catalysis of residues Glu214 and His57, previously...
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Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... TRa1proteinwasfractionated on a polyacrylamide gel and visualized by an image analyzer. The arrowindicates the band of human TRa1protein.(C) Reporter luciferase activities induced by T3. HeLaTR and ... induced DNA fragmentation and impaired theproliferation of rat FRTL cells. This implies that T3 inducesapoptosis, which is mediated by 4-1BB, and subsequentactivation of caspases in the tissues ... demonstratethat hTRa1wasactivatedbyT3intheHeLaTRcellsandstimulated the transcription of genes in a TRE-dependentmanner and that the parental HeLa cells were TR-negative and thereby not responsive to...
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Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
... identified at )158 and )186, within E1b, and at ) 69 and ) 79, within E2 [4]. Moreover, in the human platelet-like K562 cell line and in human uterine tissue,transcription initiation sites were ... proteins Gq and G11 in vivo and is activated by the isoprostane 8-epi prostaglandin F2 alpha.J. Pharmacol. Exp. Ther. 281, 95 7 96 4.36. Janssen, L.J. (2001) Isoprostanes: an overview and putative ... 17 397 –17403. 29. Lianos, E.A. & Bresnahan, B.A. ( 199 9) Effect of thromboxane A2inhibition and antagonism on prostaglandin and leukotrienesynthesis in glomerular immune injury. J. Laboratory Clin. Med....
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Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf
... ( 199 6) Analysis of main chaintorsion angles in proteins: prediction of NMR coupling cons-tants for native and random coil conformations. J. Mol. Biol. 255, 494 –506.27. Wu¨thrich, K. ( 198 6) ... in two positions, 12 and 15, at which the native amino acidsL-phenylalanine 12 and L-leucine 15 have been replaced by the nonprotein amino acidsD-phenylalanine and a-aminoisobutyric acid (Aib), ... &Kuhar, M.J. ( 198 5) Differential regulation of corticotropin-releasing factor receptors in anterior and intermediate lobes ofpituitary and in brain following adrenalectomy in rats. Neurosci.Lett....
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Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1) potx
... specificbinding sites for the lyn kinase [31]. These findings provide arationale for investigating whether caspase- mediated path-wayscaninteractwithPDE5inintactcells. In this article, we show that caspase- 3 ... molecularmass of 93 98 kDa [5]. The enzyme is phosphorylated at S92 and activated by both protein kinase A and protein kinase G[6–7]. Here we explore the possibility that PDE5A1 may beregulated by caspase- 3 ... in am-matory mediator production [ 19, 20]. Caspases are synthes-ized in the cell as inactive proenzymes. These are activated by proteolysis at internal sites and are subdivided intoinitiators and...
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Báo cáo khoa học: Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities pptx
... h at 37 °C and subsequently analyzed by SDS/PAGE and silver staining. The metalloproteinase inhibitors EDTA,o-phenanthroline (PHEN) and TIMP-1 and the thiol protease inhi-bitor E64 and serine ... MS analysis of human GCP-2 aftercleavage by gelatinase B. Human recombinantGCP-2 was analyzed by electrospray ion trapMS before (A) and after (B) incubation withactivated natural gelatinase ... processing of other chemo-kines by gelatinase B, human GCP-2 was incubated withnatural gelatinase B from human neutrophils at an enzymeto substrate ratio of 1 : 10. SDS/PAGE analysis showedthat...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... [6,7],FERM and PDZ-domain-containing 2 (FRMPD2) [8] and Ras guanine exchange factor (RasGEF) very-KIND (v-KIND, or kinase noncatalytic C-lobedomain containing 1) [9] . The KIND domain in theseproteins ... growth and branching of cerebellargranule cells and hippocampal neurons.Among the KIND domains in four KIND-containingproteins (Spir-2, PTPN13, FRMPD2 and v-KIND),only the KIND2 domain of v-KIND ... for the interactionbetween v-KIND and MAP2. (A) Alignment of the KIND2-1 region in human, mouse and Gallus (top) and the corresponding mousev-KIND KIND1, Spir-2 KIND and PTPN13 KIND domains (bottom).The...
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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... celllines. When deciding between maintaining cells alive orinducing apoptosis, caspase function and activationare regulated in terms of which pathways induce cleav-age sites and modification ... and Bad bind 14-3-3,the phosphoserine ⁄ threonine binding proteins in thecytosol. 14-3-3 prevents Bax and Bad dissociation fromtranslocating to the mitochondria by a conformationchange, and ... proteins. Furthermore, manykinases and phosphatases are cleaved by activatedcaspases. The initiator caspase- 9 has several sites thatare phosphorylated by multiple protein kinases [88],including...
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