... lowerthan that of isomaltase, respectively. The Kmfor a- pNPG of Mun/Bpu was the same a s that of isomaltase, whereas the Kmfor a- pNPG of M un/Bst was about 50 times l ower thanthat of isomaltase. ... Val216 decides the substrate specificity of a- glucosidaseinSaccharomyces cerevisiaeKeizo Yamamoto1, Akifumi Nakayama2, Yuka Yamamoto1,* and Shiro Tabata11Department of Chemistry, Nara ... between the a- glucosidic linkages of a- 1,4and a- 1,6.McCarter and Withers [26] indicated that Asp214 on the consensus region II of maltase is the catalyticnucleophile. Because the Asp214 of maltase...