Báo cáo khoa học: Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3 pot

... Recombinant expression of an insulin-like peptide 3 (INSL3) precursor and its enzymatic conversion to mature human INSL3 Xiao Luo1, Ross A. D. Bathgate2 ,3 , Ya-Li Liu4, ... before and after in vitro refolding. (B) Far-UV spec-tra of the mature human INSL3 and porcine insulin.Fig. 7. cAMP activity of recombinant INSL3 and its precursor com-pared to synthetic INSL3. ... and folded INSL3 was  80%, suggesting thatthe INSL3 precursor refolded efﬁciently in vitro. Enzymatic conversion of the single-chain INSL3 precursor into mature INSL3 To convert the INSL3 precursor...

Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf

... The use of mutant (e.g. deletion and extracopy numbers) and transgenic (RNAi and overexpres-sion) lines will help us to better understand therelationship between Hsps and cold tolerance.Experimental ... shock fac-tor mutant is accompanied by increased expression of Hsp 83 and DnaJ-1. Physiol Genomics 25, 4 93 501. 23 Bettencourt BR, Hogan CC, Nimali M & Drohan BW(2008) Inducible and constitutive ... effects of carbon dioxide anesthesia and anoxia on rapidcold-hardening and chill coma recovery in Drosophilamelanogaster. J Insect Physiol 52, 1027–1 033 .64 Hoffmann AA, Anderson A & Hallas...

Báo cáo khoa học: Mechanistic investigation of a highly active phosphite dehydrogenase mutant and its application for NADPH regeneration pptx

... Interact 1 43 144, 533 –542.41 Dunning Hotopp JC, Auchtung TA, Hogan DA &Hausinger RP (20 03) Intrinsic tryptophan ﬂuorescenceas a probe of metal and alpha-ketoglutarate binding to TfdA, a ... of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S.Mathews Ave, IL 61801, USAFax: +1 217 33 35052Tel: +1 217 33 32 631 E-mail: zhao5@uiuc.eduW. A. van ... Thehigher stability of the complex of WT PTDH and NAD is most likely due to the contributions of thehydrogen bonds between Glu175 and the 2¢- and 3 -hydroxyl groups of the adenine ribose of NAD thatare...

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

... tissues of rabbits at different time points (n =3) Tissues 2w 4w 6w Lungs 22.67±1. 53 18.67±1. 53 11.67±1. 53 Liver 26.67±1. 53 19.67±1. 53 13. 33 1. 53 Bonemarrow 40.00±4 .36 29.00±1.00 23. 33 1. 53 Normal ... Thera-peutic angiogenesis for patients with limb ischaemia by autol-ogous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial. Lancet. 2002 Aug 10 ;36 0( 933 1):427 -35 3. ... 12.67±1. 53 9.67±0.58 6 .33 ±0.58 Necrotic femoral head 26 .33 ±0.58 49 .33 ±2.52*# 66 .33 3. 51*# Note: * P<0.05 vs 2 w; # P<0.05 vs 2 w and 4 w. Discussion At present, intravenous transplantation...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... b-bar-rels of domains A and B (Fig. 2B), and had six hydro-gen bonds, three to the main chain carbonyls at Asn6,Leu168, and Val170, and three to the side chains of Arg11, Arg71, and Tyr216.The ... (Ala1 93, Leu297, Leu3 63, Phe371, Trp3 73, Ile427, Val428,Leu 430 , Trp508, and His509) and one hydrophilic resi-due, Asp 236 , form the binding pocket. The most evi-dent difference between MaL and ... laccase;proton transfer; redox potentialCorrespondenceN. Hakulinen, Department of Chemistry,University of Eastern Finland, JoensuuCampus, P.O. Box 111, FIN-80101 Joensuu,FinlandFax: +35 8 13 25 133 90Tel:...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... protein content, resultingin atomization temperatures of 230 0 °C and 2150 °C formanganese and iron, respectively. L-tryptophan-containingstandards were used to simulate the protein background.In ... manganese analogue [4] of the iron containing class I RNR of Escherichia coli. Thisassignment was based on an analysis of its metal compo-sition and similarity of its absorption spectrum to di-manganese(III) ... incorpo-ration of iron into R2F in vivo, even in the absence of manganese, and it is the availability of manganese thatis the limiting factor determining the amount of func-tional metalloradical cofactor...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... kinetics and mechanisms of the reactions of iron(III) with b-dik-etones. The formation of monoacetylacetoatoiron(III) and the effect of copper (II) on the formation of monothenoyltriﬂuoroacetonatoiron ... Germany) calibrated with Fe, Zn, Cu, Ni and Mnstandards in the range £ 500 lgÆL)1. The wavelengths usedwere 238 .204 nm (Fe), 2 13. 856 nm (Zn), 32 4.754 nm (Cu), 231 .604 nm (Ni), and 259 .37 3 nm ... the recent addition of several new proteinstructures (Protein Data Bank identiﬁers of selectedexamples: 2PFW, 1VJ2, 2OZJ, 3ES1, 3EBR, 3FJS,3ES4, 3D82, 3BCW, 3CEW, and 3CJX) to the data-base...

Tài liệu Báo cáo khoa học: Complete reconstitution of an ATP-binding cassette transporter LolCDE complex from separately isolated subunits docx

... of E. coli is com-posed of one copy each of membrane subunits LolC and LolE, and two copies of ATPase subunit LolD [7].Both LolC and LolE are assumed to span the mem-brane four times and to ... University of Tokyo,1-1-1 Yayoi, Bunkyo-ku, Tokyo 1 13- 0 032 ,JapanFax: +81 3 5841 8464Tel: +81 3 5841 7 830 E-mail: htokuda@iam.u-tokyo.ac.jp*Present addressDepartment of Biological Mechanisms and Functions, ... RI and HindIII, and then cloned into the same site of pMAN885EH. To construct pNASE carrying lolE under PBAD,a1 .3 kbp EcoRI–PstI fragment of pKM301 [10] was clonedinto the same site of pMAN885EH.To...

Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherﬁsh Misgurnus fossilis Functional consequences for the enzyme ppt

... enzyme can be chan-ged by affecting rates of transcription and ⁄ or transla-tion and ⁄ or mRNA and protein degradation. Thisrepresents a quantitative strategy to offset the lack orexcess of kinetic ... very often leads to chan-ges in two main traits of some metabolic enzymes:quantitative and qualitative.The quantitative properties (concentration and, as aconsequence, total activity) of an ... detailed understanding of the functional and metabolic consequences, further study needs to identify the kinetic, thermodynamic and regulatoryproperties of recombinant LDH-Aa4 and LDH-Ab4homotetramers...

Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx

... like to thank L. Mayer and K. Uchida for pro-viding antibodies, A. do Vale, F. Pisarra and M.T. Sil-va for help and comments on TEM, and M. Santos and R. Hultcrantz for critical reading of the ... Sciot R, Moirand R, Jego P,Leroyer P & Brissot P (19 93) Regulation of ferritin and transferrin receptor expression by iron in human hepa-tocyte cultures. J Hepatol 18, 30 1 31 2. 32 Hagen K, ... inﬂuenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, suchas MHC class I and II, CD1d, ICAM-1 and the novelCD8 ligand gp180. We...

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