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Báo cáo khoa học: Deviation of the neurosporaxanthin pathway towards b-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene ppt

Báo cáo khoa học: Deviation of the neurosporaxanthin pathway towards b-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene ppt

Báo cáo khoa học: Deviation of the neurosporaxanthin pathway towards b-carotene biosynthesis in Fusarium fujikuroi by a point mutation in the phytoene desaturase gene ppt

... fujikuroi by a point mutation in the phytoene desaturase gene Alfonso Prado-Cabrero1, Patrick Schaub2, Violeta Dı´az-Sa´nchez1, Alejandro F. Estrada1,Salim Al-Babili2and Javier Avalos11 ... manufacturer’s instructions.Two microlitres of cDNA were used for the amplification of carB using the primers 5¢-ATGAGCGACATTAAGAAATCTG-3¢ and 5¢-CTAATTCGCAGCAATGACAAG-3¢. The PCR was performed using 500 ... upstream of the start codon and the first 957 bp of the carB coding sequence, and 5¢-CGTTGAGGCACTGGTTAACG-3¢ and 5¢-CGAGAATCATGGACATAGAC-3¢, covering the last coding 1048and 88 bp downstream of the...
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Báo cáo khoa học: Transcription of mammalian cytochrome c oxidase subunit IV-2 is controlled by a novel conserved oxygen responsive element pptx

Báo cáo khoa học: Transcription of mammalian cytochrome c oxidase subunit IV-2 is controlled by a novel conserved oxygen responsive element pptx

... reverseCTCGCGGGCTCGGCAGTGGGAGPprom+1AGTCTATTCTCGAGCACCTGGGACTACAGGPprom+2AGTCTATTCTCGAGCCCAAAGCGCTGAGATTACAGPprom+3AGTCTATTCTCGAGATGCTTCTGGAGTAGGAGGCAPprom+4AGTCTATTCTCGAGGTGTGGAGGAGGCAGGGAGACPprom+5AGTCTATTCTCGAGGAGGCGCTCTGCAGTGCCTCPprom+6AGTCTATTCTCGAGAAGCAGGACGTTCCCACGCTGPprom+7AGTCTATTCTCGAGGGGGCGGGCGCCCGCACTCAGPpromreverseAGTCTATTCTCGAGCGCGACCTGGGTCTGCCCAGPORE ... EMSA.Primer ID Sequence (5’- to 3’)P-1cowTCTTGCGGCTTGGAGAGAGCCAGP-2cowCCAGAACGCGACCCAGGTCP-3cowCAGGTCTGCAGAGCAAGCAACAGP-1ratTAGTTGCAAGCTGAAGACCGP-2ratGCTGAAGACCGCGGAGGTACP-3ratGAGGTACCCAGAACTGCCCTGP-1mouseGATAGTCAGTGGGGGAAACCTCAGP-2mouseCAGCAAAAGAGGGCTGTGTGGTGP-3mouseTGGCCGCCACGAACATCCCATCPpromoter ... IIIfowardATATTCTAGGATCCTGGCTCATTCACTGCTGTCACPintron IIIreverseATATTCTAGGATCCCGGCTTCCCCCTCCCTGCAGPHIF- 1a mutGCAAATTCTTACTGAGCTTTTACTATATGCACAGCPOREGGACGTTCCCACGCTGGGGPORE mutGGTCGTAACCACGCTGGGGPSp1GGCTGTGGGGCGGGCCGGPSp1...
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Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc

Báo cáo khoa học: Maturation of Pichia pastoris-derived recombinant pro-Der p 1 induced by deglycosylation and by the natural cysteine protease Der p 1 from house dust mite doc

... 15¢)GGGGAGCTCTTAGAGAATGACAACATATGG-3¢pPICZaA5¢cloning primer mature Der p 1 5¢)GGGCTCGAGAAAAGAACTAACGCCTGCAGTATCAAT-3¢Sequence primers used for vector pPICZaA5¢AOX, 3¢AOX and a- factor primer672 ... (data not shown).Proteolytic cleavage of recombinant pro-Der p 1As autocatalytic cleavage was not achieved, enzymaticallyactive natural Der p 1 was evaluated as a tool to inducematuration of ... DIGGlycan Differentiation Kit (Roche Diagnostics GmbH,Mannheim, Germany) using the following lectins: Galanthusnivalis agglutinin, S ambuc us nigra agglutinin, Maackiaamurensis agglutinin, peanut...
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Báo cáo khoa học: Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand pptx

Báo cáo khoa học: Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand pptx

... TACAATGTACCGGAGATCTATTGATCATGTTACATGGCCTCTAGATAACTAG3 TACAATGTACCGAAGATCTATTGATCATGTTACATGGCTTCTAGATAACTAG4 TACAATGTACCGTGGATCTATTGATCATGTTACATGGCACCTAGATAACTAG5 TACAATGTACCGAGAATCTATTGATCATGTTACATGGCTCTTAGATAACTAG6 TACAATGTACCGAmAGATCTATTGATCATGTTACATGGCTTCTAGATAACTAG7 ... TACAATGTACCG2GGATCTATTGATCATGTTACATGGCTCCTAGATAACTAG10 TACAATGTACTCGAAGCTATCTATTGATCATGTTACATGAGCTTCGATAGATAACTAG11 TACAATGTATCATGAAGTACTCTATTGATCATGTTACATAGTACTTCATGAGATAACTAG12 TACAATGTATCGCGAAGCGCTCTATTGATCATGTTACATAGCGCTTCGCGAGATAACTAG13 ... TACAATGTACCGAmAGATCTATTGATCATGTTACATGGCTTCTAGATAACTAG7 TACAATGTACCGmAAGATCTATTGATCATGTTACATGGCTTCTAGATAACTAG8 TACAATGTACCGmAmAGATCTATTGATCATGTTACATGGCTTCTAGATAACTAG9 TACAATGTACCG2GGATCTATTGATCATGTTACATGGCTCCTAGATAACTAG10...
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Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx

Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx

... that the cell-bound and -generated FXIIa and kallikrein areprotected from inactivation by endogenous inhibitors in the plasma. Activation of PPK plays a primary role in initiatingand maintaining ... cleavage product of HK generated by proteolytic attack by kallikrein generated by activation of PPK as a consequense of vessel injury. The activation of FXIIand PPK/HK may start with a local increase ... comparedto inactivating FXIIa in the medium. The inactivationonly slightly affected the dissociation of FXIIa fromHUVEC indicating that C1-INH inactivates cell-boundFXIIa by binding to FXIIa...
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Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

Tài liệu Báo cáo khoa học: Phosphorylation of cyclin dependent kinase 4 on tyrosine 17 is mediated by Src family kinases pptx

... 3¢-GTCACAGAGCCACAGTTCCAGCCAGGA-5¢. Codon 305 of C-YES was mutated fromAAA (Lys) to AGA (Arg) with the forward primer: 3¢-GGAACCACGAAAGTAG CAATC AGA ACACTA AAACC A GGTACAATGATGC-5 ¢ . All vector inserts ... 96-wellplate format assay. Each sample contained 2.5 lg of total protein.Values are the average of four samples with error bars indicating the SEM.Phosphorylation of CDK4 by Src family kinases ... ⁄cyclin D kinase activity is com-monly deregulated in cancer. The majority of cancerscontain at least one genetic alteration that affects the RB pathway [7], and a better understanding of howCDK4...
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Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx

... enhanced in the presence of IREBinding to RNA and ATPase activity are characteris-tics of RNA helicases, and RNA binding increases the ATPase activity of these proteins [26]. To test whether the ... IRP-1, again increasing IRE-bindingactivity. It is proposed that the H2O2-mediated conver-sion from cytosolic aconitase to an IRE-bindingprotein is a result of a signaling pathway rather than of ... Site-directedmutational analysis for the ATP binding of DnaA pro-tein. Functions of two conserved amino acids (Lys-178and Asp-235) located in the ATP-binding domain of DnaA protein in vitro and in vivo. J Biol...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... a major contaminant of eEF 1A protein can be excluded by MALDI TOF analysis of the Coomassie blue bandextract [21] and by the absence of a correspondingnucleolin signal in western-blotting of ... oligodeoxy-nucleotides interacting with the SH2 domain of Stat3, a protein encoded by a proto-oncogene that is activa-ted in many human cancer cells, represent a novel class of aptameric therapeutic agents for the ... presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the proteinwas incubated with 1 ng of the indicated probes. After30 min incubation at room...
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Báo cáo khoa học: Mechanisms of amyloid fibril self-assembly and inhibition Model short peptides as a key research tool pptx

Báo cáo khoa học: Mechanisms of amyloid fibril self-assembly and inhibition Model short peptides as a key research tool pptx

... crucial role in the fibrillization process [27]. The results of a systematic alanine-scan of a shorterIAPP fragment (Table 1) indicated that other thanphenylalanine, any amino acid within the fragmentcould ... important informa-tion of the role of aromatic moieties in amyloid fibrilformation [36–41]. A parameter-free model based on the mathematical analysis of many peptide fragmentsand their analogues ... because of a gradualincrease in life expectancy. The genuine understanding of the mechanisms leading to the formation of amyloidfibrils and its inhibition is therefore of high clinicalimportance....
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Báo cáo khoa học: Folding of epidermal growth factor-like repeats from human tenascin studied through a sequence frame-shift approach pdf

Báo cáo khoa học: Folding of epidermal growth factor-like repeats from human tenascin studied through a sequence frame-shift approach pdf

... 1020304050600102030405060area (%)area (%)time (min) A Fig. 4. Oxidative folding kinetics. (A) Disappearance of the startingproduct (%, area of the initial reduced form with respect to the totalintegrated area) ... repeats display-ing a different pattern in the cysteine spacing. The oxidative folding of frame-shifted peptides simulates, in a way, the mispairing that would occur whether inter-rather than intra-repeat ... structureelements. A qualitative analysis of the spectra suggests the absence of helical structure, and a dominant component of irregular structure in all the peptides. A quantitative analysis of secondary...
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