... 15¢)GGGGAGCTCTTAGAGAATGACAACATATGG-3¢pPICZaA5¢cloning primer mature Der p 1 5¢)GGGCTCGAGAAAAGAACTAACGCCTGCAGTATCAAT-3¢Sequence primers used for vector pPICZaA5¢AOX, 3¢AOX and a- factor primer672 ... (data not shown).Proteolytic cleavage of recombinant pro-Der p 1As autocatalytic cleavage was not achieved, enzymaticallyactive natural Der p 1 was evaluated as a tool to inducematuration of ... DIGGlycan Differentiation Kit (Roche Diagnostics GmbH,Mannheim, Germany) using the following lectins: Galanthusnivalis agglutinin, S ambuc us nigra agglutinin, Maackiaamurensis agglutinin, peanut...