Báo cáo khoa học: Thiamin diphosphate in biological chemistry: new aspects of thiamin metabolism, especially triphosphate derivatives acting other than as cofactors pdf

... MINIREVIEW Thiamin diphosphate in biological chemistry: new aspects of thiamin metabolism, especially triphosphate derivatives acting other than as cofactors Lucien Bettendorff and Pierre WinsGIGA-Neurosciences, ... adenylate kinase; AThTP, adenosine thiamin triphosphate; TenA, thiaminase II; ThDP, thiamin diphosphate; ThDPase, thiamin diphosphatase; ThMP, thiamin monophosphate; ThMPase, thiamin monophosphatase; ... hydrolyzed to thiamin + Piby a thiamin monophosphatase (ThMPase). Thiamin maythen be pyrophosphorylated to ThDP by thiamin diphosphokinase (thiamin + ATP « ThDP + AMP;EC 2.7.6.2). Thiamin can be...

Tài liệu Báo cáo khoa học: Catabolite repression in Escherichia coli – a comparison of modelling approaches docx

... glucose and a number of other carbohydrates(Fig. 1). It consists of two common cytoplasmic pro-teins, enzyme I (EI) and histidine-containing protein(HPr), as well as an array of carbohydrate-speciﬁcenzyme ... composed of EIIA, B and C domains, with the EIIA and B do-mains being part of the phosphorylation chain and theEIIC domain representing the membrane domain. As all components of the PTS, depending ... changes of model components. A problem may occur when notthe masses per se but the concentration (mass of acompound based on a certain volume, or mass of acompound based on the entire biomass as...

Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... inhibitor in the substrate-binding groove of the kinase, whereas the SH2 domaininteracts with phosphorylated tyrosine residues of theIR kinase loop, which help position the PIR–BPS andincrease binding ... andtankyrase 2 [15]. The binding of ZIP to Grb14, byrecruiting protein kinase Cf, enhances the serine phos-phorylation of Grb14 and its inhibitory effect on IRkinase and insulin signaling [10]. ... quan-titated by measurements of speciﬁc [125I]insulin binding(0.02 nm) as previously described [17].Assay of IR tyrosine kinase activityThis assay was based on the ability of IR to phosphorylateRCAM-lysozyme,...

Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf

... unusual in havingtwo histidine residues (rather than the His-Asn pairseen in OYE, MR and some of the other memberproteins; Fig. 1B) in the active site for ligand andsubstrate binding. For ... contain aHis-His pair, there has been no report of a systematicanalysis of the contribution of each histidine residue tobinding and catalysis. Thus, to ascertain the role of each histidine ... residues in PETN reductaseinferred to be involved in ligand binding andcounterpart residues in related enzymes andalso the location of the tyrosine residueimplicated as proton donor in OYE.Table...

Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

... exclude insulin as causing the basal activation of MAP-kinases; especi-ally as we found that a substantial degree of phos-phorylation of the insulin receptor and IRS1 wasrequired to increase the ... with indicated concentration of insu-lin for 10 min. Mean ± SE, n ¼ 5 subjects. In this and the followingﬁgures, the insulin-stimulated effect was obtained by setting thevalue with no insulin ... maximal effects of insulin on the state of tyrosine-phosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ERK1 ⁄ 2, was not signiﬁcantlydifferent in either group of diabetics...

Báo cáo khoa học: Antibacterial peptides in stimulated human granulocytes Characterization of ubiquitinated histone H1A doc

... rate w as 6 mLámin)1and fractions of 6 mLwere collected. The dashed line indicates the gradient of acetonitrilecontaining 0.1% (v/v) tri¯uoroacetic a cid. The line with asterisksindicates ... (1±2%) than in histone H2A(10%) [13,14]. Recently, ubiquitination of histone H3 wasreported [15]. The covalent linkage of ubiquitin targets mostproteins for proteasome degradation, whereas the ... tissue of the Asian toad [10], respectively.All h istones are basic proteins, containing a relatively largeamount of lysine and/or arginine. Several post-translationalmodi®cations of histones...

Báo cáo khoa học: Structural features in the C-terminal region of the Sinorhizobium meliloti RmInt1 group II intron-encoded protein contribute to its maturase and intron ppt

... free-living cultures of S. meliloti strain RMO17 containing plasmids encoding thewild-type or mutant RmInt1, as described previouslyFig. 4. Effect of mutations in the C-terminal region of the RmInt1 ... IIintron-encoded proteins are multifunctional and contain an N-terminalreverse transcriptase domain, followed by a putative RNA-binding domain(domain X) associated with RNA splicing or maturase ... forprotein interactions with the high-afﬁnity binding site in subdomain DIVa of the intron, whereas other regions of the RT and domain X interact with con-served catalytic core regions [12]. Domain...

Báo cáo khoa học: Intermodule cooperativity in the structure and dynamics of consecutive complement control modules in human C1r Structural biology docx

... in the same bufferand eluted by application of an increasing ionic-strengthgradient. Both fragments were veriﬁed by MS.The concentration of recombinant proteins was deter-mined by measuring ... con-tacts, like in C1r CCP2, may contribute to fold stabil-ity in mannose-binding lectin-associated serineprotease (MASP)1 ⁄ 3 CCP1, MASP2 CCP1 and CCP2,and C1s CCP2, whereas in C1r CCP1 and ... orforming pH-dependent interaction sites on CCP1.Surface turns and loops with increased mobility canprovide easily variable protein–protein interaction sites in proteins. In CCP1, the A–B turn has...

Báo cáo khóa học: Furanocoumarin biosynthesis in Ammi majus L. Cloning of bergaptol O-methyltransferase ppt

... mega-sperma f. sp. glycinea), have been used in the past, and at least in case of the induction of parsley cells the eliciting principlehas been identified as a peptide [10]. Furanocoumarins arepotentially ... B5+-medium in 250 mL flasks) were initiated and growncontinuously in the dark as described elsewhere [1,2]. PmgFig. 1. Schematic outline of linear furanocou-marin biosynthesis. The sequence of hydroxy-lations ... purification was monitored by SDS/PAGE [30] andenzyme activity assays of individual fractions.Enzyme assays and other analytical methodsBMT activity was routinely measured at 42 °C in 200 mMpotassium...

Báo cáo khoa học: "Multiple Interpreters in a Principle-Based Model of Sentence Processing" potx

... fined by allowing non-terminal daughters to dominate a recursive instance of the schema. It is interesting to note that, for phrase structure at least, the relevant principles of grammar can ... par(ial input, as illustrated by the operation shown in Figure 2. For the partial input "What did John put ~, we can recover the partial phrase structure, including the trace in Infl 2. In ... analysis are made incrementally (for each input item) on the basis of principles which are intended max- imise the overall interpretation. We have dubbed this the Principle of Incremental Comprehension...

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