... structure with domain N acting as a connector. By contrast, TVAI adopts a monomeric structure with domain N, a starch-bindingdomain, acting as an anchor in the catalytic reaction of the enzyme, ... found to bind to domain N, suggesting that domain N also functionsas a pullulan-binding domain.AbbreviationsACA, acarbose; BNPL, neopullulanase from Bacillus stearothermophilus; D35 6N, mutant ... recognition mech-anism of starch, but that of pullulan containing a- (1,6)glucosidic linkages is still unclear.TVAI has an N- terminal distorted b-barrel domain(domain N) , in addition to three conserved...