Báo cáo khoa học: Roles of prolactin-releasing peptide and RFamide related peptides in the control of stress and food intake potx

... MINIREVIEW Roles of prolactin-releasing peptide and RFamide related peptides in the control of stress and food intake Yuki Takayanagi and Tatsushi OnakaDivision of Brain and Neurophysiology, ... progress in research investigating the roles of these two peptides in the control of energy metabolism and stress. PrRPPrRP was considered to serve as a hypothalamic-releasing factor and to act on the ... hypothalamus and the anterodorsalthalamic nucleus. Roles of RFRP in the control of food intake and stress responses The dorsomedial hypothalamus plays an importantrole in the control of energy...

Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt

... and transduction of the measurement of galactose to the nucleus through protein–protein interaction leading togene expression. The status of the switch is determined by the state of the upstream ... element of the GALgenes in the nucleus. The regulatory protein may bind to the upstream regulatory element constituting a protein–DNA interaction [4–6]. The availability of the regulatoryproteintointeractwithDNAmaydependonitsactivity,which ... from the candidatemodels that best describe the input–output experimentaldata. T he individual components of the selected modelprovide insights into the importance of their roles in the overall...

Báo cáo khoa học: A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion docx

... SH3domain in the same protein, and that this intramolecu-lar interaction suppresses the lipid-binding activity of the PX domain in the resting state; phosphorylation of p47phoxreleases the binding, ... domainsplay important roles in the function of proteins contain-ing this domain [40]. In particular, p47phox, which con-tains the PX domain and two SH3 domains, has beenintensely studied. In ... PXdomain to phosphoinositides might be regulated by the interaction between the PX domain and the third SH3domain, as in p47phox, although whether the interaction of the PX domain with the third...

Báo cáo khoa học: Human ATP-dependent RNA ⁄ DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP ppt

... mayABFig. 5. The role of the C-terminal, HBXIP interacting, domain of hSuv3p in protein stability. (A) The protein stability of the wildtype hSuv3TAP and the hSuv3TAP variant lacking the C-terminal 136 ... decline in the truncated protein levelswithin 4 h following inhibition of protein synthesis bycycloheximide. We next wanted to examine whether the presence of HBXIP binding protein fragment of ... cycloheximide to the culture medium. The protein levels were examined by Western blot in the indicated time points. (B) The protein stability of the fusion protein containing the C-ter-minal 136 amino acids...

Báo cáo khoa học: Afﬁnity puriﬁcation-mass spectrometry Powerful tools for the characterization of protein complexes ppt

... beinvolved in signaling processes related to the regulation of synaptic plasticity by binding to the adaptor proteinPSD95. This protein contains several PDZ domains one of which binds to the ... C-terminal amino acids of the NMDA receptor. This particular feature wasexploited for the retrieval of a PSD95-containing complexby immobilizing a hexapeptide corresponding to the C-terminus of the ... advantages for the analysis of protein complexes both in terms of dynamic range (i.e. howlittle of one protein can be identiﬁed in the presence of howmuch of a mixture of other proteins) and absolute...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... chaincorresponding to the catalytic domain (C domain); and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked ... of the pro-tein in the N state and the evolution of the overallstructure of the protein in the MG state. This degree of resolution is unprecedented for the T domain of the diphtheria toxin. ... [21]. For the T domain, these were the center of TH5 and TH8, and the N-terminal half of TH9. Alla-helices (TH1, 3, 4, 5’, 6, 7 and the remaining parts of TH5 and TH9) except for TH2 showed intermediateprotection...

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... striking feature of the structure is an inherent asymmetry among the catalyticb subunits. In combination with the asymmetric loca-tion of the central c subunit, and in accordance with the binding ... toclarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investigated whether the c subunits from the sodium F-ATP ... incubation of 2 lg of pure c11with 2 lgofc1puriﬁed in detergent, the slower migrating bandreappeared (lanes 4 and 8). Upon incubation of 2 lg of pure c11with 2 and 10 lgofc1puriﬁed in...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... role of AP-2a and AP-2e in chondrogenesis. Overview of the differen-tiation stages during chondrogenesis and the involvement of transcription factors(henatoxylin and eosin-stained section of ... helix–span–helix domain at the C-terminus shown in blue medi-ates dimerization and, together with the basic domain, (red) DNA-binding. ‘*’, amino acids that are identical in all sequences in the align-ment; ... apoptosis [73]. The authors of this studyascribe the regulation of outgrowth of limb buds and patterning of the digits to the chicken AP-2. The role of AP-2a was further studied in zebraﬁsh.It...

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the HSF2 ... would enhance or diminish the impact of HSF2 deﬁciency.Pending questions for the roles of HSF1 and HSF2 in oogenesis and in early embryos The role of HSF2 in oogenesis and in pre-implantationdevelopment ... reduced methylation of the histone H3K9, irre-spective of the relative location of the HSF4-bindingregions and of the transcriptional status of geneslocated around the HSF4-binding regions. This...

Tài liệu Báo cáo khoa học: Roles of the human Rad51 L1 and L2 loops in DNA binding doc

... permonomer of poly(dT), showing that the changes weremainly due to the binding of the ﬁrst DNA, while the binding of the second DNA had less in uence(Fig. 5A). To ensure that the estimation of the ... located, and are not found at the ATP-binding siteor the subunit–subunit interface of the Rad51 ﬁlament.Therefore, the Rad51 L1 and L2 loops may also beinvolved in DNA binding. In the present ... ssDNA binding, but notdsDNA binding [22], in contrast to the case of the ABFig. 5. Fluorescence changes of tryptophan probes inserted in the L1 and L2 loops of Rad51 upon poly(dT) binding. The...

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