Báo cáo khoa học: Sodium pump a1 and a3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma docx
Báo cáo khoa học: Sodium pump a1 and a3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma docx
... Sodium pump a1 and a3 subunit isoforms mediate distinct responses to ouabain and are both essential for survival of human neuroblastoma Larisa Karpova1,2, Alexander Eva1, Ulrike ... the a1ora3 subunit orthrough both subunit isoforms of the sodium pump. Ouabain- induced activation of Erk1⁄2 aftersilencing either a1ora3 subunitsCells expressing only the ubiquitous a1 subunit ... thoselacking a3; however, the impact of this is not known.Nevertheless, the results clearly show that both a1 and a3 subunits are essential for survival, and that the a1 and a3 isoforms have distinct...
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Tài liệu Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference pptx
... Wang1 and Dongyun Hao1,21 Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, China2 Biotechnology Research Centre, Jilin Academy of ... has beenproven to be the recognition site of DRE-bindingproteins, which are transcription factors that haveauthentic ERF domains [13] and that are involved inthe induction of rd29A expression ... important implications for understanding the foundations of recognition of agiven binding site by divergent members of the AtERFfamily.Results and DiscussionThe members of the ERF family in...
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Tài liệu Báo cáo khoa học: Pyrimidine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus – biochemical characterization and homology modeling doc
... times of adenosine and adenine, inosine and hyp-oxantine, guanosine and guanine, uridine and uracil, cyti-dine and cytosine were 12.2 and 6.2 min, 10.5 and 4.7 min,15.2 and 6.1 min, 6.8 and ... [58,59] and FUGUE [60] and gave the same results, therefore confirm-ing the correctness of the chosen templates. The sequences of target and templates were aligned to each others and to those of the ... [39].Hyperthermophilic Archaea are of extreme interest for understanding the molecular mechanisms of structural and functional adaptation of proteins to extreme tem-peratures and also for the peculiar substrate...
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Tài liệu Báo cáo khoa học: Neuropeptide Y-family receptors Y6and Y7in chicken Cloning, pharmacological characterization, tissue distribution and conserved synteny with human chromosome region docx
... studies. Both were found thanks to their sequence similarity to other Y receptors, and the sequence comparisons sug-gested that both Y6 and Y7arose before the radiation of gnathostomes in ... the epitope tag (not shown). To avoidhaving to rely on a high-affinity radioligand for deter-mination of the receptor’s pharmacological profile, weperformed a number of functional assays to determinewhether ... receptorprobably required the N-terminal region of the ligands for high-affinity binding. Mammalian Y2receptorsseem to be unique among all Y receptors in their abil-ity to bind truncated NPY and...
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Tài liệu Báo cáo khoa học: Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes docx
... in the areas of morphology of the respiratory system, its adaptations and the physics of respiration [1–3]. By contrast, the molecularmechanisms of tracheole ventilation are poorly understood.Early ... dilution of biotinylated anti-rabbit IgG (VectorLaboratories) for 1 h. Tissues were washed 4 · 20 min inPBSTG and incubated in a 1 : 200 dilution of Texas Red-Streptavidin (Vector Laboratories) for ... ontopofgradientsandultracentrifugationwasperformedat100 000 g for 16 h at 5 °C. Calibration curves for thedetermination of the apparent sedimentation coefficientwere constructed using cytochrome...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... constitutive punctae formation and Ca2+influx. In addition,the grafted single EF-hand motif formed a dimer regardless of the presence of Ca2+, which conforms to the EF-hand paring paradigm. ... conformation, whereas the b-strandcontent largely remained similar to CD2.D1 (Fig. 1E).The isolated EF-hand binds to Ca2+ and lanthanide ionsOne of the most important steps to fully understandthe ... dissociationconstant for Tb3+, and [P]T and [M]T are the total concen-trations of protein and Tb3+, respectively. The Ca2+competition data were first analysed to derive the apparentdissociation...
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Báo cáo khoa học: Insulin induces heme oxygenase-1 through the phosphatidylinositol 3-kinase/Akt pathway and the Nrf2 transcription factor in renal cells pptx
... factor,hypoxia-inducible factor-1 (HIF-1), mediates essential homeostatic responses to reduced oxygen [43,44].HIF-1 has been shown to mediate transcriptional acti-vation of HO-1 in a rat model of hypoxia [45] and ... phos-phatidylinositol 3-kinase (PI3K) pathway and itsdownstream effectors [23]. This pathway is a key sig-nal transducer of many growth factors and cytokines and has been implicated in the regulation of cellgrowth, ... 1.5 h. Nuclear and cytosolic lysateswere prepared and analyzed by western blotting using antibody to Nrf2, with loading control with b-actin for cytosolic extracts and lamin A ⁄ C for nuclear extracts....
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Báo cáo khoa học: DYNLL/LC8: a light chain subunit of the dynein motor complex and beyond pptx
... checkpoints and itis involved in recruitment of checkpoint proteins to dynein [77].Apoptosis/autophagyBimL and Bmf are BH3-only pro-apoptotic proteinsthought to be normally sequestered to dynein and ... complex crucial for receptor immo-bilization at both excitatory and inhibitory synapses.Scaffold proteins constitute one major group of pro-teins present at the PSD and two of them are LC8binding ... implications for design and use of multivalent ligands and inhibi-tors. Angew Chem Int Ed 37, 2754–2794.38 Barbar E, Kleinman B, Imhoff D, Li M, Hays TS &Hare M (2001) Dimerization and folding of...
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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... loading controls, respectively, and also used to ascer-tain the purity of the nuclear and cytosolic preparations (A). Bands were scanned, quantified densitometrically and are expressed as arbitraryunits ... values are depicted after normalization of PEPCK and G6Pase bands with those of 18S rRNA(B). Each point is the mean ± SEM of three sets of experiments. (C) Real-time PCR quantification of PEPCK and ... series of events beginning with the TNFa-mediated prevention of the effect of insulin on Foxa2 localization and lead-ing to the abrogation of insulin inhibition of gluconeo-genesis and glucose...
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Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc
... 3.5 · more expression of both the S688A(3.5 · for PCSK9 and 3.2 · for its propeptide, respec-tively; lanes 1A and 1B) and E690A PCSK9 mutants(3.4 · for PCSK9 and 3.5 · for its propeptide, respec-tively; ... cell cultures of HepG2, Huh7, HEK293 and CHOK1 cells untranfected and transfected with ahPCSK9 expression vector were collected and immuno-precipitated as above for TOF-MS analysis of immuno-captured ... sera (B,D), and following dephosphorylation (C, D).(E–H) TOF-MS analyses of the molecularforms of the propeptide of V5-taggedPCSK9 immunoprecipitates from the media of transfected and overexpressing...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)QUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ