... equation v is the initial velocity, [E]0 the enzymeconcentration, [S] the substrate concentration, [I] the inhibitor concentration, Km the Michaelis constant andK1i, K2i, L1i, L2i the ... added to the buffer compartment of the control and to the compartment containing AMY1 in the sample cell. The AMY1 concentration in the control cellwas adjusted accordingly by addition of buffer. ... 1). Thus the longer the substrate, the higher was the activity. Inhibition by acarboseInhibition of amylose hydrolysis occurred in the presence of 10–80 lMacarbose and the association constants...