... Jolla, CA,USA) with pET2 8a( +)-wild-type Ec DosH as a templateand using the following respective 5¢-sense primers: 5¢-gatgagtcgggagACCcagctggagaaaaaag-3¢,5¢-gatgagtcgggagTTTcagctggagaaaaaag-3¢,5¢-ggacccgttttgcgACCtcgaaagtgagc-3¢,and ... decreased the rate of auto-oxidation [5],whereas Ala and Asn substitutions at Asp40, anamino-acid residue that interacts via two water mole-cules with the proximal ligand His77, markedlyincreased ... domain are intra-molecularly transduced to regulation of the catalyticdomain [6]. Specifically, the X-ray crystal structure of the isolated heme-bound PAS domain (Ec DosH)Keywordsauto-oxidation;...