Báo cáo khoa học: Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium – implications on substrate specificity and catalysis pptx
... 21.7
– – – – 336 75.6
– – – – 337 61.7
– – – – 338 3.4
Table 2. Angles C4–O4¢–C1¢ produced by two glucose residues in the CD molecules. –, not applicable.
Numbers of the two glucose residues
1–2 ... P2
1
2
1
2
1
(TvuCMBP–G4) and C2
(TvuCMBP–a-CD and TvuCMBP–b-CD) crystals were
grown at 20 °C under almost the same buffer conditions.
Although the...
... procedures
Enzyme production and crystallization
Gene cloning and the production, purification and crystalli-
zation of TtAKb-Thr were performed as previously
described [31]. TtAKb-free was crystallized by ... electron densities of the N-termi-
nal (residues 1–3 in chain A, residues 1–4 in chains B
and C, and residues 1–5 in chains E–G) and C-termi-
nal (residues 15 8–1...
... key features of PDH specificity and
mode of inhibition of the enzyme.
Results and Discussion
Structural analysis and model quality
Crystal structures of three different complexes of
LlPDH have ... compilation ª 2006 FEBS
Crystal structures of a bacterial 6-phosphogluconate
dehydrogenase reveal aspects of specificity, mechanism
and mode of inhibition by analogu...
... network of loops
connecting the strands is complicated; six loops con-
nect b-strands from both b-sheets ( 1–1 ¢ 1 –2 2–2 ¢ 5 –3
5–6 ¢ 6 –6 ) and five loops connect b-strands within the
same b-sheet (2 –3 ¢ ... of reaction leading to retention of configura-
tion on the anomeric carbon of the b-(2,1)-
D-fructofuranose.
Fig. 5. Dimer of b-fructofuranosidase from...
... the HR2 regions).
Results and Discussion
Structure determination
The HR1 and HR2 regions of the NiV and HeV F
proteins consist of residues 13 7–1 78 and residues
45 3–4 85, respectively, and were predicted ... The
A
B
C
Fig. 1. Construction and sequence alignment of the Nipah virus
(NiV) and Hendra virus (HeV) fusion cores. (A) Prediction of the
heptad repeat (HR) re...
... Photographs and electron density maps of the SUMO-2 crystals. Shown in (A) and (B) are two different crystal forms I and II obtaine d under
slightly different conditions. The sizes of crystals are ... respectively,
corresponding to about o ne-quarter and more than one-
third of the contact surfaces. T he interactions include two
hydrogen bonds between backbone atoms of Gly27...
... with the
conformation of active-site residues. However, mutations of
Arg8 and Glu21 are rarely observed in resistant isolates [2].
One of the conformations of the side chain of Lys45 in PR
forms ... chain of Met90¢ has two conformations,
and one conformation has a short separation from the carbonyl oxy-
gen of the catalytic Asp25¢.(B)ComparisonofMet90¢ in PR
L90M
and
L...
... 1).
Results
Binding of AMA and odorants in solution
The dissociation c onstants of the AMA–OBP c omplexes fo r
the bovine and porcine OBP isoforms were, respectively, 1 .0
and 1.5 l
M
and the corresponding ... K
diss
for AMA and the
concentration of AMA.
Crystallization, data collection and refinement
of bovine OBP
Except for the bOBP–UND complex, bOBP crystals were
obtaine...
... mgÆmL
)1
of protein, 30% (w ⁄ v) PEG 1000, and 0.1 m of
Tris ⁄ HCl at pH 8.5. Refinement of preliminary crystalliza-
tion conditions resulted in the growth of quality crystals in
a solution containing ... volume of reservoir solu-
tion at 500 lL and the drop volume at 5 lL, containing
2.5 lL of protein sample and 2.5 lL of reservoir solution.
AeKAT crystals were obtained in...