... Hydroxyproline, hydroxy-lysine and phosphoserine were not detected in WSM, WIM,EDTA-SM and EDTA-IM.The comparison of the amino acid composition of themain peak obtained from AE-HPLC of WSM and ... presence of residual EDTA afterintensive dialysis of EDTA-SM was also observed in aminoacid composition determination as EDTA eluted near to thehistidine, precluding from detecting this amino acid. ... [41]. In the presentstudy, most of the proteins from both EDTA-SM andWSMmigratedinthegelwithnodistinctpattern,leavingadark continuous smear after silver staining (Fig. 4). Nodiscrete individual...