Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
Báo cáo Y học: Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting docx
... Expression of uncoupling protein-3 in subsarcolemmal and intermyofibrillar mitochondria of various mouse muscle types and its modulation by fasting Maria Jimenez, Cedric Yvon, Lorenz ... differently affected by metabolic changes.Keywords: fasting; intermyofibrillar mitochondria; muscle type; subsacorlemmal; uncoupling protein-3 (UCP3).The first uncoupling protein described, uncoupling ... levels of COX protein were not significantly different in IMF and SS mitochondria. The yield in mitochondria, which wasdetermined by comparing the level of COX protein in thesum of the two mitochondria...
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Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx
... development of obesity by both, increasing energy expenditure and d ecreasing in situlipogenesis. In order to further analyse consequences of respiratory uncoupling in white fat, the eects of the ... UCP1 in conversion of unilocu lar into multilocular cells has notbeen studied.Reduction of adiposity by respiratory uncoupling in adipocytes may be limited by mitochondrial oxidativecapacity. ... adipocytes in transgenic animals wasoccupied by mitochondria, as com pared with only 9.6% in control animals.Finally, in order to con®rm that respiratory uncoupling in adipocytes may stimulate mitochondrial...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... protein. Most of the protein was expressed in inclusion body. The fusion protein in inclusion body was purified by using High-Affinity Nickel Iminodiacetic Acid Resin and refolded in urea gradient ... inactivation by dipeptidyl peptidase IV and by renal clearance [5, 6, 13]. Many attempts have been made to increase its biological half-life and its efficacy in vivo by producing dipeptidyl peptidase ... most of the fusion protein was in inclusion body (Fig. 2A). Western blot using mouse anti-His-tag monoclonal antibody also proved that majority of fusion protein was present in inclusion body...
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Báo cáo y học: "Expression of hMSH2 protein of the human DNA mismatch repair system in oral lichen planus"
... visualized by applying 0.01% diaminobenzidine tetrahydrochloride and 0.03% H2O2. Sections were counterstained with Meyer’s haematoxylin and mounted in PermountTM. Negative controls consisted of ... hPMS2 and GTBP/hMSH6 are very important in distinguishing and repairing misparing and slippage errors in DNA synthesis. The mismatch is detected by two complexes, the hMSH2-GTBP/hMSH6 and the ... omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis...
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Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... classes.Role of Tyr183 in catalysis In previous crystallographic studies it was shown that thehydroxyl side chain of Tyr183 is within hydrogen bondingdistance of the N-acetyl carbonyl oxygen of the ... N-terminal methionine residue resultingfrom the cloning procedure has apparently been cleaved offduring the maturation of the e nzyme.Enzyme activity studiesThe lysozyme activities of the various ... Relative lysozyme activity of hevamine and hevamine mutantsat pH 5.0. ND, no detectable activity.Hevamine variant Relative activity (%)Wild-type hevamine 123Recombinant hevamine 100Tyr183Phe...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
... trp1,lys2–801, leu2D1, his3D200, pep4::HIS3, prb1D1.6R,can1, GAL] [10]. The YN11 strain was derived fromYPH499 strain, and YN45 strain was prepared by the use of YN11 and BJ5458 strains.Preparation ... in group 1 and 2 proteins. Theresults we obtained indicate that these two domains mayplay a critical role in complementability. The genesAACEVAPD1 and 3 were constructed in another yeast expression ... point, and studies by disruption of the VMA11 gene are now in progress.Isoforms of V-ATPase subunits have so far been reported in higher plants as reviewed by Sze et al. [1], three isoformsof...
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Báo cáo Y học: Expression and purification of the recombinant subunits of toluene/ o-xylene monooxygenase and reconstitution of the active complex potx
... PRISM(http://www.graphpad.com).Single turnover assaySingle turnover assays of the individual components(10nmolofTomoH,20nmolofTomoCandTomoDsubunits) and of each of their possible combinations, wereperformed by adding ... colorimetrically by complexation with Ferene S [10], or Ferrozine [21].Inorganic sulfide content was determined by methyleneblue formation as described by Rabinowitz [22] and Brumby [23], with a minor ... mass of about 10, 38 and 57 kDa, theexpected molecular size of recombinant subunits B, E and A, respectively. The identity of the proteins was furtherchecked by N-terminal sequencing of the...
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Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc
... similarity to the bovine carboxylase. Expression of this cDNA in COS cells or insect cells yielded vita-min K-dependent carboxylase activity and vitamin K-dependent epoxidase activity. The recombinant ... site of the c-carboxylase have not beendefined with certainty by affinity labeling and site-specificmutagenesis [15–20]. Cysteine residues are important withinthe active site [21], and Cys99 and ... AF382823).Comparison of the primary structure of theConus and vertebrate vitamin K-dependent carboxylasesThe N-terminal amino acids of the Conus carboxylase aredominated by acidic residues including three...
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Báo cáo Y học: Expression pattern in the antennae of a newly isolated lepidopteran Gq protein a subunit cDNA potx
... after 40 cyclesconsisting of 1 min at 94 °C, 1 min at 44 °Cand1minat72 °C in a Hybaid thermocycler. Subcloning in pZERO(Invitrogen) using EcoRI and BamHI resulted in loss of apart of the amplified ... pathway may be implicated in transduction of olfactory signals in lepidoptera. Such a hypothesis hasalready been proposed for insects in a variety of speciesusing kinetics based methodology (reviewed ... guaninenucleotide-binding proteins: the G proteins. G proteinsplay a central role in a wide variety of signal transductionpathways, mediating the perception of environmental cues in all higher eukaryotic...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... of a coating, by releasedpenaeidin, of the bacteria before their internalization intopenaeidin-devoid hyaline cells. Finally, the diversity, thelarge distribution and abundance of penaeidins ... the site of injectionInjection of microorganisms resulted in a dramatic decrease in numbers of both circulating and tissue in ltratinghaemocytes within 3 h of injection. To study haemocytebehaviour ... and analysed by in situ hybridization using antisense pen-3ariboprobes labelled with DIG-UTP and by immunocytochemistryusing an anti-penaeidin Ig detected by secondary antibody labelledwith...
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