... Hishinuma2, Mitsuo Mita2 and Takashi Morita1Departments of 1Biochemistry; and 2Pharmacodynamics, Meiji Pharmaceutical University, Tokyo, JapanIn this study, we isolated a 25-kDa novel snake ... doi:10.1046/j.1432-1033.2002.02940.xpurchased from Seikagaku Corporation (Tokyo, Japan).Other chemicals were of analytical grade (Sigma–Aldrich,Amersham–Pharmacia Biotech., Wako Pure Chemical Ind. and Kanto Chemical Co.).Purification ... Cloning and characterization of novel snake venom proteinsthat block smooth muscle contractionYasuo Yamazaki1, Hisashi Koike1, Yusuke Sugiyama1, Kazuko Motoyoshi1, Taeko Wada1,Shigeru...