0
  1. Trang chủ >
  2. Luận Văn - Báo Cáo >
  3. Báo cáo khoa học >

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

... pair: 5¢-AATACCAAAGAAGCCTACAATG-3¢ and 5¢-GTACGAAGTGGAGGTATGTCATC-3¢.bPrimer pair: 5¢-GCCATTTTAGGTGCTATTCTGG-3¢ and 5¢-TATTTTCTTTATCTGAGTTTA-3¢.cPrimer pair: 5¢-CATCCATAGCAGATAACAGTC-3¢ and ... Two alternativepoly (A) signals [A( 1259)TAAA and A( 1430)ATTAAA]giving rise to poly (A) tails were observed by PCR-screening of the bovine mammary gland cDNA library.The N-terminal amino-acid ... RasmussenProtein Chemistry Laboratory, Department of Molecular and Structural Biology, University of Aarhus, DenmarkThe present work reports isolation and characterization of a highly glycosylated protein...
  • 9
  • 614
  • 0
Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

... nucleospinextract k it (Macherey±N agel), then digested by NcoIandBamHI, and cloned i nto the pET-8c vector.Screening of a cDNA library A kgt11 cDNA library of Chlamydomonas was a gift fromM. ... protein and with BSA(400 lgámL)1). The reactions were stopped by adding3.3 mMEDTA and analyzed on an agarose gel.Thioredoxin-dependent peroxidase activity of Ch-Prx1Peroxidase activity assays ... thioredoxin-dependentperoxidase fromChlamydomonas reinhardtiiAymeric Goyer1, Camilla HaslekaÊs2, Myroslawa Miginiac-Maslow1, Uwe Klein2, Pierre Le Marechal3,Jean-Pierre Jacquot4 and Paulette Decottignies31Institut...
  • 11
  • 608
  • 0
Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Interestingly, P II was the only isoform withaffinity for rhamnose. As a result, P II lacked thegalactose and ⁄ or N-acetyl galactosamine specificitythat is a characteristic feature of the archetypal type ... galactose and galactose-containing structures, can agglutinatehuman and rabbit erythrocytes, and kills mice and themicrocrustacean Artemia salina at very low concentra-tions [10]. Similar to the ... inhibited by galactose and its deriva-tives [such as N-acetylgalactosamine (GalNAc),methyl -a- d-galactopyranoside], it was evident that, atdoses up to 100 mm, glucose, mannose, a- methylman-noside,...
  • 12
  • 763
  • 0
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx

... 5¢-TGAACCATGCTCTATGGCAAAATCAATACCATC-3¢Asp125Asn Sense strand 5¢-TTGGATGGTATTGATTTTAACATAGAGCATGGTTCAACC-3¢Anti-sense strand 5¢-GGTTGAACCATGCTCTATGTTAAAATCAATACCATCCAA-3¢Glu127Ala Sense strand 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢Anti-sense ... 5¢-CAGGGTTGAACCATGCGCTATGGCAAAATCAATACCATC-3¢Tyr183Phe Sense strand 5¢-TATGTATGGGTTCAATTCTTTAACAATCCACCATGCCAG-3¢Anti-sense strand 5¢-CTGGCATGGTGGATTGTTAAAGAATTGAACCCATACATA-3¢Asp125Ala/Tyr183Phe This mutant was ... 5¢-GGTATTGATTTTGACATAGCGCTATGTCAAAATCAATACC-3¢Anti-sense strand 5¢-GTACAGGGTTGAACCATGCGCTATGTCAAAATCAATACC-3¢Asp125Ala/Glu127Ala Sense strand 5¢-GATGGTATTGATTTTGCCATAGCGCATGGTTCAACCCTG-3¢Anti-sense strand 5¢-CAGGGTTGAACCATGCGCTATGGCAAAATCAATACCATC-3¢Tyr183Phe...
  • 9
  • 616
  • 0
Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... Kawakami B, Yamano H,Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala-nine chloride-lyase (deaminating) of Pseudomonas putidaCR 1–1. Purification and characterization of a novel enzyme ... pyridoxal-5¢-phosphate (PLP)-dependent enzymesthat catalyse elimination and replacement reactions of amino acids have been purified and characterized [6].Keywords1-aminocyclopropane-1-carboxylatedeaminase; Arabidopsis ... sequence, such as N-glycosylation,tyrosine sulfation, phosphorylation, myristylation, and amidation sites, all of them are characterized by a highprobability of occurrence.Enzyme activity of the recombinant...
  • 14
  • 565
  • 0
Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx

... 5¢-ACAAGGGTAGACCAAACACCAAGAACAGCAACAAAAGAGACGGGCGAATCACTGACCATCAACgccGTCCTGAGAGAT-3¢) and N8518 (Reverse: 5¢-TTTCACGGTTAATGCGGTGCCAGCTCCCCAACTGTAATAAATACCAGACAAATTATATGCTCCaacCCTATACGTGCCACTG-3¢); followed by secondary PCR using VNARterminaloligonucleotide ... variant of 12F-11 incorporating mutations Cys22Ala and Cys82Val was constructed by overlapping PCR usingoligonucleotide primers N8517 (Forward: 5¢-ACAAGGGTAGACCAAACACCAAGAACAGCAACAAAAGAGACGGGCGAATCACTGACCATCAACgccGTCCTGAGAGAT-3¢) ... the isolation and characterization of a VNARthat binds with high affinityto human Tom70 as an important demonstration thatVNARlibraries can provide novel binding reagents againstrefractory...
  • 12
  • 522
  • 0
Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

Báo cáo Y học: Expression and characterization of recombinant vitamin K-dependent c-glutamyl carboxylase from an invertebrate, Conus textile doc

... Conus carboxylase and vertebrate vitamin K-dependent c-carboxylasesargue for conservation of a vitamin K-dependent carb-oxylase across animal species and the importance of c-carboxyglutamic acid ... (Grand Island, NY, USA). RACE kits and Advantage cDNA polymerase mix were from Clontech(Palo Alto, CA, USA). AmpliTaq Gold polymerase and buffer were from Perkin–Elmer (Branchburg, NJ, USA).Reagents ... carboxylase activity. Further, usinginsect cells, this expression system provides a source of Conus carboxylase free of endogenous carboxylase and carboxylase substrates.Molecular mass analysis...
  • 11
  • 537
  • 0
Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

Báo cáo Y học: Cloning and characterization of novel snake venom proteins that block smooth muscle contraction pptx

... Hishinuma2, Mitsuo Mita2 and Takashi Morita1Departments of 1Biochemistry; and 2Pharmacodynamics, Meiji Pharmaceutical University, Tokyo, JapanIn this study, we isolated a 25-kDa novel snake ... doi:10.1046/j.1432-1033.2002.02940.xpurchased from Seikagaku Corporation (Tokyo, Japan).Other chemicals were of analytical grade (Sigma–Aldrich,Amersham–Pharmacia Biotech., Wako Pure Chemical Ind. and Kanto Chemical Co.).Purification ... Cloning and characterization of novel snake venom proteinsthat block smooth muscle contractionYasuo Yamazaki1, Hisashi Koike1, Yusuke Sugiyama1, Kazuko Motoyoshi1, Taeko Wada1,Shigeru...
  • 8
  • 472
  • 0
Báo cáo Y học: Identification and characterization of thioredoxin and thioredoxin reductase fromAeropyrum pernixK1 pdf

Báo cáo Y học: Identification and characterization of thioredoxin and thioredoxin reductase fromAeropyrum pernixK1 pdf

... Trx/TRsystem of the aerobic archaea.MATERIALS AND METHODSMaterialsThe plasmid pET-3d was purchased from Novagen(Madison, WI, USA). KOD DNA polymerase and T4DNA polymerase were purchased from ... Identification and characterization of thioredoxin and thioredoxinreductase fromAeropyrum pernixK1Sung-Jong Jeon and Kazuhiko IshikawaNational Institute of Advanced Industrial Science and Technology ... Industries(Tokyo, Japan). Other reagents were of the reagent gradeavailable.Cloning and expression of A. pernix TRX and TRChromosomal DNA of A. pernix K1 was prepared asdescribed by Sako et al. [21]....
  • 8
  • 414
  • 0
Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

Báo cáo Y học: Cloning and characterization of the mammalian-specific nicolin 1 gene (NICN1) encoding a nuclear 24 kDa protein doc

... and characterization of the mammalian-specific nicolin 1gene (NICN1) encoding a nuclear 24 kDa proteinBianca Backofen1,*, Ralf Jacob2,*, Katrin Serth3, Achim Gossler3, Hassan Y. Naim2 and ... XhoI/SacI subfragment fromthe murine IMAGE 1349406 clone and T7-RNA poly-merase (Roche, Mannheim Germany). A sense RNA probeusing T3-RNA Polymerase (Roche, Mannheim, Germany)was used as a negative ... consists of 213 amino acids and has a calculated molecular weight of 24 kDa (Fig. 1). The aminoacid sequences of the NICN1 proteins are 94% identicalbetween human and dog, and 89% identical betweenhuman...
  • 6
  • 450
  • 0

Xem thêm

Từ khóa: chuyên đề điện xoay chiều theo dạngMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)