Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... state, the < /b> CaN autoinhibitory domain lies over the < /b> catalytic site[20]. The < /b> amino-acid sequences of < /b> the < /b> CnAa and < /b> CnAb catalytic domains are 90% identical, and < /b> the < /b> autoinhibitorydomain amino-acid ... substrates, the < /b> phosphatase activities of< /b> CaNa and < /b> CaNb are only 70%, and < /b> 50% inhibited by< /b> 90 lMCaP, respectively. Inhibition < /b> of < /b> CaNa and < /b> CaNb phosphatase activity by < /b> FKBP12 /FK506 < /b> or < /b> CypA/CsA The < /b> structurally ... Substrate < /b> selectivity < /b> and < /b> sensitivity < /b> to < /b> inhibition < /b> by < /b> FK506 < /b> and < /b> cyclosporin < /b> A < /b> of < /b> calcineurin < /b> heterodimers < /b> composed < /b> of < /b> the < /b> a < /b> or < /b> b catalytic subunit Brian A.< /b> Perrino1, Andrew J. Wilson2,...
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Báo cáo khoa học: Caspase-2 is resistant to inhibition by inhibitor of apoptosis proteins (IAPs) and can activate caspase-7 pot
... caspase-2 activity than the < /b> yeast death assay.Caspase-2 is not inhibited by < /b> mammalian IAPproteinsMembers of < /b> the < /b> mammalian IAP family contribute to< /b> the < /b> regulation of < /b> apoptotic pathways in part ... activation of < /b> caspase-7. Caspase-2 was not subject to < /b> inhibition < /b> by< /b> members of < /b> the < /b> IAP family of < /b> apoptosis inhibitors.AbbreviationsAFC, 7-amino-4-trifluoromethyl coumarin; CARD, caspase activation and < /b> ... 5¢-GAGAATTCTCAGTCCATCCCATTTCTGGC-3¢.Yeast transformation and < /b> death assaysSaccharomyces cerevisiae strain W30 3a < /b> was used in all yeasttransformation and < /b> death assays, as described previously[26,31].Preparation of < /b> yeast lysatesFor...
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Báo cáo y học: "Relationships between free radical levels during carotid endarterectomy and markers of arteriosclerotic disease"
... the < /b> samples treated with an antioxidant cocktail from that of < /b> the < /b> saline samples yields the < /b> part of < /b> the < /b> signal that can be attributed to < /b> superoxide and/< /b> or < /b> hydroxyl radicals or < /b> any secondary ... via the < /b> facial vein into the < /b> jugular vein and < /b> advanced up to < /b> the < /b> level above the < /b> skull base, and < /b> the < /b> facial vein was divided. The < /b> catheter was advanced 12-15 cm up until resistance and < /b> then ... and < /b> Alice Wallenberg founda-tion. We want to < /b> thank Anita Fae for excellent labora-tory and < /b> Elisabeth Ståhlgren for excellent secretarial work. CONFLICT OF < /b> INTEREST The < /b> authors have declared...
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Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot
... 5¢-GCGCTCGAAAATCTATTCAAGAACACC-3¢At173anti 5¢-GTTCTTGAATAGATTTTCGAGCGCACCGATGATAAC-3¢Bn173sense 5¢-GCCCTAGAAAATCTATTCAAGAACACC-3¢Bn173anti 5¢-GTTCTTGAATAGATTTTCTAGCGCACCATT-3¢At399sense 5¢-GCCGGAGGCAGAGCCGTGATCGAT-3¢At399anti ... was measured by< /b> incubation with malonyl CoA and < /b> required cofactors,followed by < /b> transacylation of < /b> the < /b> CoA conjugates to < /b> methylesters [34,39]. Elongation activity of < /b> both A.< /b> thaliana and< /b> B. napus ... substrate< /b> specificity and < /b> therefore substrate < /b> binding because the < /b> fattyacyl CoA substrates are expected to < /b> be delivered from the< /b> cytosol and < /b> available at the < /b> membrane interface [1]. This isin contrast to < /b> membrane-bound...
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Tài liệu Báo cáo Y học: Transactivation domains are not functionally conserved between vertebrate and invertebrate serum response factors potx
... GATA-4 [16] or< /b> myocardin [17] transcription factors. Other cofactors areregulated by < /b> growth-factor transduction pathways, such as the < /b> classical ternary complex factors, that are activated by< /b> MAP-kinase ... 2002TCTTATATGAATGCAGTTCTG-3¢;N3:5¢-GGGAATTCGCTAGGCCACAGTTTGAATTTG-3¢;N4:5¢-GGGAATTCGACCTCTGAAAATGTAAAACAG-3¢;N5:5¢-GGGAATTCGGATCCTCTAACTGGGTTAGAT-3¢;N6: 5¢-GGGAATTCGTCCCCGGACGAGGACAGGTCA-3¢;N7:5¢-GGGAATTCGCCTGCCAATGGTAAAAAGACA-3¢.TheC1deletionwasgeneratedbyPCRusingtheoligonucleotide ... of< /b> transcription factors that also include the < /b> animal MEF-2factor and < /b> a < /b> large number of < /b> plant transcription factors[3,4]. Immediately C-terminal to < /b> the < /b> MADS box there is a< /b> region of < /b> amino acids (SAM-domain)...
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Báo cáo Y học: Variations in receptor site-3 on rat brain and insect sodium channels highlighted by binding of a funnel-web spider d-atracotoxin pdf
... association rate. The< /b> binding of< /b> 125I-labelled d-ACTX-Hv 1a < /b> to < /b> rat brain syna-ptosomes is inhibited c ompetitively by < /b> classical ScaTxs and< /b> allosterically by < /b> brevetoxin-1, similar to < /b> ScaTx binding.However, ... E-mail: dgordon@post.tau.ac.ilAbbreviations: Aah-II, antimammalian a-< /b> toxin II from the < /b> venom of< /b> the < /b> scorpion Androctonus australis hector; d-ACTX-Ar1, d-atraco-toxin-Ar1 ( formerly robustoxin) ... depolarizedconditions.Affinity of < /b> d-ACTX-Hv 1a < /b> for polarized and < /b> depolarizedrat brain synaptosomes To < /b> study the < /b> influence of < /b> synaptosome membrane potentialon125I-labelled d-ACTX-Hv 1a < /b> affinity,125I-labelledd-ACTX-Hv1a...
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Báo cáo Y học: Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases pot
... GC-MS as described above and < /b> on a < /b> radio-GCconsisting of < /b> a < /b> Carlo-Erba 4160 Series gas chromatograph(Carlo-Erba, Milano, Italy) equipped with a < /b> RAGA-90radioactivity detector (Raytest, Straubenhardt, ... primer for B9 3 contained a < /b> PstI restriction site5¢-GCCAACTGCAGAATGAGGCGATCTGCCGATTACG-3¢. The < /b> sense primer for C62 and < /b> M34 was5¢-GCCAGGATCCAATGAGGAGATCAGCAAACTACC-3¢, containing a < /b> BamHI restriction ... E.F. & Maniatis, T. (1989) MolecularCloning: a < /b> Laboratory Manual, 2nd edn. Cold Spring. HarborLaboratory Press, Cold Spring Harbor, New York.30. Bouwmeester, H.J., Wallaart, T.E., Janssen,...
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Báo cáo Y học: Boar salivary lipocalin Three-dimensional X-ray structure and androstenol/androstenone docking simulations pot
... Universite´di Pisa, Italy The < /b> X-ray structure of < /b> variant A < /b> of < /b> authentic boar salivarylipocalin (SAL), a < /b> pheromone-binding protein specificallyexpressed in the < /b> submaxillary glands of < /b> the < /b> boar, has beensolved ... excreted, they are strongly associated withspecific sex pheromones. Proteins similar to < /b> the < /b> urinaryproteins are also produced by < /b> the < /b> salivary glands and < /b> by< /b> nasal glands of < /b> both sexes of < /b> the < /b> same species ... conformational changes. Interestingly, theseresidues are Val and < /b> Ala, respectively, in isoform A < /b> of < /b> SAL, the < /b> structure of < /b> which is described here, and < /b> are replaced inisoform B by < /b> Ala and < /b> Val,...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... polyacrylamide) followed by < /b> electrotransfer to < /b> a< /b> nitrocellulose membrane. Blots were incubated with a< /b> 32P end-labelled MREa probe as described in Materials and < /b> methods and < /b> subjected to< /b> autoradiography. ... extracts from HepG2 for 5 min and< /b> then incubated for an additional 30 min either in the < /b> absence (lane 1) or < /b> presence (lane 2, 3) of < /b> varying concentrations of < /b> mAb 162. Bandsshifted by < /b> the < /b> antibodies ... copper transport. Hepaticcirrhosis and < /b> neuronal degeneration are the < /b> most debilita-ting symptoms of < /b> WD and < /b> are caused by < /b> the < /b> impairment of< /b> biliary copper excretion and < /b> the < /b> accumulation of < /b> toxicconcentration...
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Báo cáo Y học: DNA supercoiling in Escherichia coli is under tight and subtle homeostatic control, involving gene-expression and metabolic regulation of both topoisomerase I and DNA gyrase docx
... and < /b> that the < /b> pA1lacO-1 promoter and < /b> the < /b> lacIq1gene are surrounded by < /b> a < /b> DNA fragmentoriginating from upstream the < /b> gyrA gene and < /b> a < /b> fragmentcontaining the < /b> N-terminal part of < /b> the < /b> gyrA gene. To < /b> ... to< /b> nitrocellulose and < /b> Ponceau staining [Ponceau-S, 0.2% in3% trichlororacetic acid (Serva)] the < /b> topoisomerase I and< /b> gyrase A < /b> bands were cut out and < /b> ground. Polyclonalantibodies were raised by < /b> Eurogentec by < /b> ... regulatory lo ops. I n metabolic contr ol ana-lysis the < /b> extent to < /b> which a < /b> parameter controls a < /b> variable isquanti®ed by < /b> a < /b> control c oef®cient. For instance for the< /b> control of < /b> aLk by < /b> topoisomerase...
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