... lM,aVmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1forSUR2A, and a Km of 41 lM,aVmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol PiÆminÆmg)1for SUR2B.A ... with estimated Vmaxvalues of 37, 26, 8 and 31 nmol PiÆminÆmg)1, and Kmvalues of 769, 556, 882 and 340 lM, respectively. (B) ATPase activity of a mixture of NBD1 and eitherNBD2A (s, n = ... estimated Kmvalues of 995 and 878 lM, and Vmaxvalues of 31 and 27 nmol PiÆmin)1Æmg)1protein, respectively. The dashed line is the average of the ATPase activities of NBD1 and NBD2B.Table...