... kidney.(Received 2 July 20 02, revised 3 September 20 02, accepted 10 September 20 02) Eur. J. Biochem. 26 9, 528 8– 529 4 (20 02) Ó FEBS 20 02 doi:10.1046/j.14 32- 1033 .20 02. 0 324 6.x [21 ] and astressin [22 ], a conformationally ... step synthesis as described [21 ,31].Synthesis and purification of peptides The CRF peptides (Fig. 1) were synthesized, purified, andcharacterized as described [ 12, 21 ,22 ]. For the synthesis of the ... specificactivity of 74 TBqÆmmol)1. Binding and cAMP assay For the determination of the binding affinity and the biological potency of the photoactivatable CRF antagonists,HEK 29 3 cell lines, stably transfected...