Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
Báo cáo Y học: Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli doc
... N-terminal sequencing and amino-acid analysis. The sequence obtained, namely Lys-Ile-Glu-Glu-Gly-Lys-Leu-Val-Ile-Trp corresponds to the N-terminus of the m ature maltose-binding protein. Binding of ... pressure and reduced exploratory activityindicating involvement of the receptor in a variety of physiological functions [6]. D irect structure determination of hA2aR by X-ray or electron crystallography, ... 2002 Puri®cation and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli H. Markus Weiû and Reinhard Grisshammer*MRC Laboratory of Molecular Biology, Hills...
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Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc
... (T in pig and human; M in rat), 614 (I in pig and human; L in rat), 680 (L in pig and human; I in rat), 760 (K in pig; R in rat and human) , 991 (A in pig and human; S in rat), 1009 (M in pig and human; ... only in their carbohydrate moiety.Analysis of the carbohydrate structures revealed that the brain enzyme contains a glycostructure of the oligoman-nose/hybrid type. The occurrence of terminal ... shown).Synthesis of the TRH-DE by hepatocytes in culture In contrast to hepatocytes kept as monolayers in primarycultures, hepatocytes cultured in a sandwich configurationcontinue to synthesize and...
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Báo cáo Y học: Purification and characterization of novel kininogens from spotted wolffish and Atlantic cod pdf
... anN-terminal heavy chain, the bradykinin moiety and aC-terminal light chain. The heavy chain and light chain areinterlinked by disulfide bridges. Kininogens are furtherclassified into high ... Most of the determined peptide sequences could be aligned with the heavy chain of human kininogen (Fig. 3), verifying the identity of these novel kininogens. The highly conservedcystatin sequence ... properties;they are substrates for kallikreins and release vasoactivepeptide bradykinin. Furthermore, they act like cystatinsinhibiting cysteine proteinases. Cod and wolffish kininogens and previously...
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Báo cáo Y học: Purification and characterization of three galactose specific lectins from Mulberry seeds (Morus sp.) ppt
... buffercontaining 0.2M galactose.Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the ... in hemagglutination, lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certainpolysaccharides and glycoproteins [1,2]. Plant lectinsisolated from a wide variety of plants ... F-1a00waseluted by the buffer containing 0.2M NaCl. Of these twofractions, only F-1a0displayed lectin activity. The fractionF-1a0might contain pure protein as it gave a single band on apolyacrylamide...
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Báo cáo Y học: Purification and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx
... VanX- and VanY-type enzymesindicated that all of these enzymes contained the same Zn2+binding motif. VanX homologues present in the cyanobac-terium Synechocystis strain PCC6803, and the glycopeptideantibiotic ... Consequently the extent of interference of precursors terminating in D-Ser on the rate of hydrolysis of UDP-MurNAc-pentapeptide[Ala]was measured using HPLC. The rate of removal of the terminalD-Ala ... examination of their Kmvalues, being 17 mM and 15 mM, respectively, asdetermined using the cadmium-ninhydrin method. HPLCanalysis of the rate of UDP-MurNAc-pentapeptide[Ala]hydrolysis in...
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Báo cáo Y học: Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt
... below.AP assaysAll assays were linear with respect to time and concentration of enzyme assayed. One unit (U) of activity is defined as the amount of AP resulting in the hydrolysis of 1 lmol of substrate ... determined with the Coo-massie Blue G-250 dye-binding method [34] using bovinec)globulin as the protein standard.Peroxidase assayA chemiluminescence assay was employed to determine the capacity ... days in the absence of exogen-ous Pihad only approximately 40% of the fresh weight of the 8-day-old +Picells (approximately 63 and 25 g of cellswere obtained per 500 mL culture of 8-day-old...
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Báo cáo Y học: Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx
... Ni2+,Mg2+,Zn2+,Cu2+ and Co2+.Partic-ularly, Cu2+ and Zn2+potently inhibited chondroitin ABClyase and chondroitin AC lyase.Heparin competitively inhibited only chondroitin AClyase, not chondroitin ABC lyase. ... analyzed the internal sequences of apeptide obtained by digestion of each enzyme with trypsin(Table 3). The internal sequences of chondroitin ABC and AC lyase show significantly greater homology ... chondroitin AC lyaseactivity containing fractions were tested for homogeneityby electrophoresis.Enzyme activity assaysChondroitin lyase activity was measured according to the following method. The...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... digested with the same enzymes. The resulting plasmid (pET28yQ) was isolated from E. coli HB101 and the identity of the ybdQ insert was confirmed byDNA sequencing. Finally, E. coli BL21(DE3)pLysS wastransformed ... UspAdomains in tandem [17]. The small members of this familyare proteins of 142–144 amino-acids long; they are acidic and presumably located in the cytoplasm like UspA.Members of this family share ... extracts using anti-GroEL Ig. It has been previouslyshown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2,18]....
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... of enzymes in the latex of Vas-concellea fruits, but in addition also results from the presence of othercysteine proteinases or isoforms. In contrast to the cysteine proteinases pre-sent in ... deg-radation, or in targeting or maturation of the enzyme,as was shown before in cathepsin C, which also belongsto the papain family of cysteine proteinases [27]. The overall similarity at the amino acid ... shown in Fig. 6, and are compared with the knownN-terminal sequences of the cysteine proteinases of C. papaya and V. cundinamarcensis. The cysteine pro-teinases of V. · heilbornii hold the generally...
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Báo cáo Y học: Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 docx
... factors [3] and their biotechnologicalpotential. Lipases have proven to be versatile enzymes in nonaqueous solvent systems in which they catalyze the synthesis of a variety of acylglycerols and specialized ... triacylglycerolscomprised of both long and medium chain fatty acids hasbeen investigated as a means of providing single substitutesfor mixed acylglycerides in dietary applications [5]. In bothhydrolysis and synthesis ... tricaprylin hydrolysis are clearly seen and correspond with the single protein band stained in native-PAGE gel. In these experiments, we found LipAshows a tendency toward aggregation, as some of the...
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